Sulfur isotope fractionation during growth of sulfate-reducing bacteria on various carbon sources

2004 ◽  
Vol 68 (23) ◽  
pp. 4891-4904 ◽  
Author(s):  
Jutta Kleikemper ◽  
Martin H. Schroth ◽  
Stefano M. Bernasconi ◽  
Benjamin Brunner ◽  
Josef Zeyer
Keyword(s):  
Isotope Fractionation ◽  
Sulfur Isotope ◽  
Carbon Sources ◽  
Sulfate Reducing Bacteria ◽  
Sulfate Reducing ◽  
Sulfur Isotope Fractionation ◽  
Reducing Bacteria

The effect of temperature on sulfur and oxygen isotope fractionation by sulfate reducing bacteria (Desulfococcus multivorans)

2020 ◽  
Vol 367 (9) ◽  
Author(s):  
André Pellerin ◽  
Gilad Antler ◽  
Angeliki Marietou ◽  
Alexandra V Turchyn ◽  
Bo Barker Jørgensen
Keyword(s):  
Sulfate Reduction ◽  
Oxygen Isotope ◽  
Isotope Fractionation ◽  
Sulfur Isotope ◽  
Sulfate Reducing Bacteria ◽  
Oxygen Isotope Fractionation ◽  
Sulfate Reducing ◽  
Sulfur Isotope Fractionation ◽  
Reducing Bacteria ◽  
Desulfococcus Multivorans

ABSTRACT Temperature influences microbiological growth and catabolic rates. Between 15 and 35 °C the growth rate and cell specific sulfate reduction rate of the sulfate reducing bacterium Desulfococcus multivorans increased with temperature. Sulfur isotope fractionation during sulfate reduction decreased with increasing temperature from 27.2 ‰ at 15 °C to 18.8 ‰ at 35 °C which is consistent with a decreasing reversibility of the metabolic pathway as the catabolic rate increases. Oxygen isotope fractionation, in contrast, decreased between 15 and 25 °C and then increased again between 25 and 35 °C, suggesting increasing reversibility in the first steps of the sulfate reducing pathway at higher temperatures. This points to a decoupling in the reversibility of sulfate reduction between the steps from the uptake of sulfate into the cell to the formation of sulfite, relative to the whole pathway from sulfate to sulfide. This observation is consistent with observations of increasing sulfur isotope fractionation when sulfate reducing bacteria are living near their upper temperature limit. The oxygen isotope decoupling may be a first signal of changing physiology as the bacteria cope with higher temperatures.

Sulfur Isotope Fractionation by Sulfate-Reducing Microbes Can Reflect Past Physiology

2018 ◽  
Vol 52 (7) ◽  
pp. 4013-4022 ◽  
Author(s):  
André Pellerin ◽  
Christine B. Wenk ◽  
Itay Halevy ◽  
Boswell A. Wing
Keyword(s):  
Isotope Fractionation ◽  
Sulfur Isotope ◽  
Sulfate Reducing ◽  
Sulfur Isotope Fractionation

Effective Removal of Lead from Solution by Sulfate Reducing Bacteria Cultured with Sugar Byproducts

2020 ◽  
Vol 14 (3) ◽  
pp. 384-395
Author(s):  
Juan Yin ◽  
Chao-Bing Deng ◽  
Hongxiang Zhu ◽  
Jianhua Xiong ◽  
Zhuo Sun
Keyword(s):  
High Efficiency ◽  
Metal Removal ◽  
Low Cost ◽  
Heavy Metal Removal ◽  
Carbon Sources ◽  
Sulfate Reducing Bacteria ◽  
Lead Removal ◽  
Sulfate Reducing ◽  
Reducing Bacteria ◽  
Filter Mud

Sulfate reducing bacteria (SRB) are widely used to remove heavy metals because of their high efficiency. However, the metabolic processes of SRB require additional carbon sources, and the development of low-cost carbon sources has gradually attracted attention. The utilization of sugar byproduct resources, as the low-cost carbon sources, has great practical significance for environmentally sustainable development in Guangxi, China. This study aims to cultivate SRB with low-cost sugar byproducts, apply them to controlling a lead-polluted environment, and study the effects and mechanisms of controlling lead pollution. The research results show that the best culture effect of SBR can be obtained by mixing the filter mud and vinasse in a ratio of 1:1 to 3:1. SRB have average lead removal rates of more than 96.97% in solutions with different lead concentration of 10∼100 mg/L, and SRB have a higher tolerance to high concentrations of lead due to factors such as the organic substance composition of sugar byproducts and the porosity of filter mud. Scanning electron microscopy combined with energy dispersive spectrometry and X-ray diffraction analysis show that SRB mainly cause Pb2+ to form PbS precipitate through redox reactions to remove lead from the solution. Therefore, low-cost filters of a mud and vinasse mixture can be used as a medium for SRB and exhibit high heavy metal removal efficiency, thus providing a new utilization of filter mud and vinasse.

scholarly journals Diversity of Sulfur Isotope Fractionations by Sulfate-Reducing Prokaryotes

2001 ◽  
Vol 67 (2) ◽  
pp. 888-894 ◽  
Author(s):  
Jan Detmers ◽  
Volker Brüchert ◽  
Kirsten S. Habicht ◽  
Jan Kuever
Keyword(s):  
Sulfate Reduction ◽  
Isotope Fractionation ◽  
Sulfur Isotope ◽  
Reduction Rate ◽  
Sulfate Reduction Rate ◽  
Dissimilatory Sulfate Reduction ◽  
Systematic Effect ◽  
Sulfate Reducers ◽  
Sulfate Reducing ◽  
Sulfur Isotope Fractionation

ABSTRACT Batch culture experiments were performed with 32 different sulfate-reducing prokaryotes to explore the diversity in sulfur isotope fractionation during dissimilatory sulfate reduction by pure cultures. The selected strains reflect the phylogenetic and physiologic diversity of presently known sulfate reducers and cover a broad range of natural marine and freshwater habitats. Experimental conditions were designed to achieve optimum growth conditions with respect to electron donors, salinity, temperature, and pH. Under these optimized conditions, experimental fractionation factors ranged from 2.0 to 42.0‰. Salinity, incubation temperature, pH, and phylogeny had no systematic effect on the sulfur isotope fractionation. There was no correlation between isotope fractionation and sulfate reduction rate. The type of dissimilatory bisulfite reductase also had no effect on fractionation. Sulfate reducers that oxidized the carbon source completely to CO2 showed greater fractionations than sulfate reducers that released acetate as the final product of carbon oxidation. Different metabolic pathways and variable regulation of sulfate transport across the cell membrane all potentially affect isotope fractionation. Previous models that explained fractionation only in terms of sulfate reduction rates appear to be oversimplified. The species-specific physiology of each sulfate reducer thus needs to be taken into account to understand the regulation of sulfur isotope fractionation during dissimilatory sulfate reduction.

scholarly journals Effects of Iron and Nitrogen Limitation on Sulfur Isotope Fractionation during Microbial Sulfate Reduction

2012 ◽  
Vol 78 (23) ◽  
pp. 8368-8376 ◽  
Author(s):  
Min Sub Sim ◽  
Shuhei Ono ◽  
Tanja Bosak
Keyword(s):  
Nitrogen Fixation ◽  
Sulfate Reduction ◽  
Isotope Fractionation ◽  
Sulfur Isotope ◽  
Isotope Effects ◽  
Reducing Power ◽  
Sulfate Reducing ◽  
Microbial Sulfate Reduction ◽  
Sulfur Isotope Fractionation ◽  
S Isotope

ABSTRACTSulfate-reducing microbes utilize sulfate as an electron acceptor and produce sulfide that is depleted in heavy isotopes of sulfur relative to sulfate. Thus, the distribution of sulfur isotopes in sediments can trace microbial sulfate reduction (MSR), and it also has the potential to reflect the physiology of sulfate-reducing microbes. This study investigates the relationship between the availability of iron and reduced nitrogen and the magnitude of S-isotope fractionation during MSR by a marine sulfate-reducing bacterium, DMSS-1, aDesulfovibriospecies, isolated from salt marsh in Cape Cod, MA. Submicromolar levels of iron increase sulfur isotope fractionation by about 50% relative to iron-replete cultures of DMSS-1. Iron-limited cultures also exhibit decreased cytochromec-to-total protein ratios and cell-specific sulfate reduction rates (csSRR), implying changes in the electron transport chain that couples carbon and sulfur metabolisms. When DMSS-1 fixes nitrogen in ammonium-deficient medium, it also produces larger fractionation, but it occurs at faster csSRRs than in the ammonium-replete control cultures. The energy and reducing power required for nitrogen fixation may be responsible for the reverse trend between S-isotope fractionation and csSRR in this case. Iron deficiency and nitrogen fixation by sulfate-reducing microbes may lead to the large observed S-isotope effects in some euxinic basins and various anoxic sediments.

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