Changes in the levels of humoral immune activities after storage of gilthead seabream ( Sparus aurata ) skin mucus

2016 ◽  
Vol 58 ◽  
pp. 500-507 ◽  
Author(s):  
Héctor Cordero ◽  
Alberto Cuesta ◽  
José Meseguer ◽  
M. Ángeles Esteban
Keyword(s):  
Sparus Aurata ◽  
Gilthead Seabream ◽  
Skin Mucus ◽  
Humoral Immune

scholarly journals Dietary Histidine, Threonine, or Taurine Supplementation Affects Gilthead Seabream (Sparus aurata) Immune Status

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1193
Author(s):  
Lourenço Ramos-Pinto ◽  
Marina Machado ◽  
Josep Calduch-Giner ◽  
Jaume Pérez-Sánchez ◽  
Jorge Dias ◽  
...  
Keyword(s):  
Immune Modulation ◽  
Sparus Aurata ◽  
Head Kidney ◽  
Building Blocks ◽  
Gilthead Seabream ◽  
Taurine Supplementation ◽  
Skin Mucus ◽  
Lectin Domain ◽  
Humoral Immune ◽  
Immune Parameters

AAs have become interesting feed ingredients to be used in functional fish feeds as not only are they protein building blocks, but they also participate in several other key metabolic processes. In the present study, a comprehensive analysis of transcriptomics, hematology, and humoral immune parameters (plasma and skin mucus) were measured twice over the course of the feeding trial (four weeks). Plasma antiprotease activity increased in fish fed Thr compared to those fed the CTRL and Tau treatments, regardless of sampling time. The bactericidal activity in skin mucus decreased in fish fed Tau and His treatments compared to those fed the CTRL diet after two weeks. The membrane IgT (mIgT) was upregulated in fish fed Tau after four weeks, while C-type lectin domain family domain 10 member (clec10a) was downregulated in fish fed Thr after two weeks of feeding. By comparing the molecular signatures of head-kidney by means of a PLS-DA, it is possible to visualize that the main difference is between the two sampling points, regardless of diet. Altogether, these results suggest that dietary supplementation with these AAs at the tested levels causes mild immune-modulation effects in gilthead seabream, which should be further studied under disease challenge conditions.

scholarly journals Differential proteome profile of skin mucus of gilthead seabream (Sparus aurata) after probiotic intake and/or overcrowding stress

2016 ◽  
Vol 132 ◽  
pp. 41-50 ◽  
Author(s):  
Héctor Cordero ◽  
Patricia Morcillo ◽  
Alberto Cuesta ◽  
Monica F. Brinchmann ◽  
María A. Esteban
Keyword(s):  
Sparus Aurata ◽  
Gilthead Seabream ◽  
Skin Mucus ◽  
Proteome Profile

scholarly journals Effect of Light–Dark Cycle on Skin Mucosal Immune Activities of Gilthead Seabream (Sparus aurata) and European Sea Bass (Dicentrarchus labrax)

Fishes ◽  
2020 ◽  
Vol 5 (1) ◽  
pp. 10
Author(s):  
Diana Ceballos-Francisco ◽  
Alberto Cuesta ◽  
María Ángeles Esteban
Keyword(s):  
Bactericidal Activity ◽  
Sparus Aurata ◽  
Dicentrarchus Labrax ◽  
Maximum Level ◽  
Sea Bass ◽  
Immunoglobulin M ◽  
Gilthead Seabream ◽  
Dark Cycle ◽  
European Sea Bass ◽  
Skin Mucus

Changes in different immune activities in the skin mucus of gilthead seabream (Sparus aurata L.) and European sea bass (Dicentrarchus labrax L.) specimens exposed to a constant light–dark photoperiod (12 h L:12 h D) were studied. Samples were collected at 08:00 (light on), 14:00, 20:00 (light off), 02:00, and again at 08:00 to determine immunoglobulin M (IgM) levels, several enzymes related to the immune system, and bactericidal activity. IgM levels were higher during the day in seabream and reached a minimum value at 20:00, but it was hardly affected in sea bass. No significant variations were recorded in the levels of protease and antiprotease. Peroxidase reached its maximum level in seabream at 02:00, the same time that it reached its minimum level in sea bass. Lysozyme showed little variation in seabream, but it was significantly lower at 14:00 than during the rest of the cycle in sea bass. Finally, different interspecific variations on bactericidal activity against Vibrio harveyi were recorded. The findings demonstrate that the immune parameters present in skin mucus of these important fish species are affected by the light–dark cycle and that there are substantial interspecies differences.

Humoral immune parameters in serum of gilthead seabream (Sparus aurata L.) after induced skin injury

2018 ◽  
Vol 75 ◽  
pp. 291-294 ◽  
Author(s):  
Diana Ceballos-Francisco ◽  
Francisco A. Guardiola ◽  
Héctor Cordero ◽  
Alberto Cuesta ◽  
M. Ángeles Esteban
Keyword(s):  
Sparus Aurata ◽  
Gilthead Seabream ◽  
Skin Injury ◽  
Humoral Immune ◽  
Immune Parameters

scholarly journals Carvacrol, Thymol, and Garlic Essential Oil Promote Skin Innate Immunity in Gilthead Seabream (Sparus aurata) Through the Multifactorial Modulation of the Secretory Pathway and Enhancement of Mucus Protective Capacity

2021 ◽  
Vol 12 ◽  
Author(s):  
Joana P. Firmino ◽  
Laura Fernández-Alacid ◽  
Eva Vallejos-Vidal ◽  
Ricardo Salomón ◽  
Ignasi Sanahuja ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Immune Response ◽  
Innate Immunity ◽  
Pathogenic Bacteria ◽  
Secretory Pathway ◽  
Sparus Aurata ◽  
Gilthead Seabream ◽  
Protective Capacity ◽  
Skin Mucus ◽  
Garlic Essential Oil

One of the main targets for the use of phytogenics in aquafeeds is the mucosal tissues as they constitute a physical and biochemical shield against environmental and pathogenic threats, comprising elements from both the innate and acquired immunity. In the present study, the modulation of the skin transcriptional immune response, the bacterial growth capacity in skin mucus, and the overall health condition of gilthead seabream (Sparus aurata) juveniles fed a dietary supplementation of garlic essential oil, carvacrol, and thymol were assessed. The enrichment analysis of the skin transcriptional profile of fish fed the phytogenic-supplemented diet revealed the regulation of genes associated to cellular components involved in the secretory pathway, suggesting the stimulation, and recruitment of phagocytic cells. Genes recognized by their involvement in non-specific immune response were also identified in the analysis. The promotion of the secretion of non-specific immune molecules into the skin mucus was proposed to be involved in the in vitro decreased growth capacity of pathogenic bacteria in the mucus of fish fed the phytogenic-supplemented diet. Although the mucus antioxidant capacity was not affected by the phytogenics supplementation, the regulation of genes coding for oxidative stress enzymes suggested the reduction of the skin oxidative stress. Additionally, the decreased levels of cortisol in mucus indicated a reduction in the fish allostatic load due to the properties of the tested additive. Altogether, the dietary garlic, carvacrol, and thymol appear to promote the gilthead seabream skin innate immunity and the mucus protective capacity, decreasing its susceptibility to be colonized by pathogenic bacteria.

Salinity influences the humoral immune parameters of gilthead seabream (Sparus aurata L.)

2005 ◽  
Vol 18 (3) ◽  
pp. 255-261 ◽  
Author(s):  
Alberto Cuesta ◽  
Raúl Laiz-Carrión ◽  
M.P. Martín del Río ◽  
José Meseguer ◽  
J. Miguel Mancera ◽  
...  
Keyword(s):  
Sparus Aurata ◽  
Gilthead Seabream ◽  
Humoral Immune ◽  
Immune Parameters

Comparative skin mucus and serum humoral defence mechanisms in the teleost gilthead seabream (Sparus aurata)

2014 ◽  
Vol 36 (2) ◽  
pp. 545-551 ◽  
Author(s):  
Francisco A. Guardiola ◽  
Alberto Cuesta ◽  
Marta Arizcun ◽  
José Meseguer ◽  
María A. Esteban
Keyword(s):  
Sparus Aurata ◽  
Gilthead Seabream ◽  
Defence Mechanisms ◽  
Skin Mucus

Proteomic profile of the skin mucus of farmed gilthead seabream (Sparus aurata)

2015 ◽  
Vol 120 ◽  
pp. 21-34 ◽  
Author(s):  
Juan Jurado ◽  
Carlos A. Fuentes-Almagro ◽  
Francisco Antonio Guardiola ◽  
Alberto Cuesta ◽  
Ma. Ángeles Esteban ◽  
...  
Keyword(s):  
Sparus Aurata ◽  
Gilthead Seabream ◽  
Proteomic Profile ◽  
Skin Mucus ◽  
Farmed Gilthead Seabream

scholarly journals Wound-Induced Changes in Antioxidant Enzyme Activities in Skin Mucus and in Gene Expression in the Skin of Gilthead Seabream (Sparus aurata L.)

Fishes ◽  
2021 ◽  
Vol 6 (2) ◽  
pp. 15
Author(s):  
Cristóbal Espinosa-Ruíz ◽  
María Ángeles Esteban
Keyword(s):  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Stress Proteins ◽  
Sparus Aurata ◽  
Gilthead Seabream ◽  
Antioxidant Enzyme Activities ◽  
Skin Mucus ◽  
Expression Levels ◽  
Induced Changes ◽  
Related Proteins

This study investigated the antioxidant enzyme activities in the skin mucus of gilthead seabream (Sparus aurata L.) at 3 and 7 days post-wounding (dpw). The expression levels of the genes that encode stress proteins (grp170, grp94, grp75, sod and hsp70) and skin regeneration-related proteins (tf, igf1, tgfb1, der1, apo1 and erdj3) in the skin also were determined. Mucus and skin samples were obtained from the left and right flanks of non-wounded and wounded fish. In both flanks of the wounded fish, catalase and glutathione reductase activities in the skin mucus increased (p < 0.05) at 3 and 7 dpw (100 ± 31% and 111 ± 25%, respectively), whereas superoxide dismutase activity increased (p < 0.05) only at 7 dpw (135 ± 15%). The expression levels of stress proteins in the skin of the wounded flank of the wounded fish mainly increased at 7 dpw (grp170 increased to 288 ± 85%, grp94 to 502 ± 143%, grp75 to 274 ± 69%, sod to 569 ± 99%, and hsp70 increased to 537 ± 14%) (p < 0.05). However, the expression levels of the tissue regeneration-related genes varied depending on the flank investigated, on the experimental time, and on the gene studied. To the best of our knowledge, this is the first work to determine the effect of a wound in different skin parts of the same fish.

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