Genetic variation in susceptibility to Phytophthora Cambivora in European chestnut (Castanea sativa)

2006 ◽  
Vol 226 (1-3) ◽  
pp. 199-207 ◽  
Author(s):  
Cécile Robin ◽  
Olivier Morel ◽  
Anna-Maria Vettraino ◽  
Charikleia Perlerou ◽  
Stephanos Diamandis ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Castanea Sativa ◽  
European Chestnut

Impact of the Asian gall wasp ( Dryocosmus kuriphilus ) on the radial growth of the European chestnut ( Castanea sativa ).

2021 ◽  
Author(s):  
Enrico Marcolin ◽  
Mario Pividori ◽  
Fernanda Colombari ◽  
Maria Chiara Manetti ◽  
Francesco Pelleri ◽  
...  
Keyword(s):  
Radial Growth ◽  
Castanea Sativa ◽  
Gall Wasp ◽  
Dryocosmus Kuriphilus ◽  
European Chestnut

Identification of plant genes involved on the initial contact between ectomycorrhizal symbionts (Castanea sativa – European chestnut and Pisolithus tinctorius)

2009 ◽  
Vol 45 (3) ◽  
pp. 275-282 ◽  
Author(s):  
Mónica Sebastiana ◽  
Andreia Figueiredo ◽  
Bartolomeu Acioli ◽  
Lisete Sousa ◽  
Fernando Pessoa ◽  
...  
Keyword(s):  
Castanea Sativa ◽  
Pisolithus Tinctorius ◽  
Initial Contact ◽  
European Chestnut ◽  
Plant Genes

scholarly journals The role of production site isolation in the plant health situation of sweet chestnut (Castanea sativa)

2017 ◽  
pp. 79-82
Author(s):  
Gabriella Kovács ◽  
László Radócz
Keyword(s):  
Castanea Sativa ◽  
Cryphonectria Parasitica ◽  
Western Slope ◽  
Past Century ◽  
Sweet Chestnut ◽  
European Chestnut ◽  
Disease Occurrence ◽  
New Perspective ◽  
Blight Disease ◽  
Health Situation

The most destructive pathogen for the European chestnut is the blight fungus Cryphonectria parasitica (Murr.) Barr. The spread of the fungus was very fast in Europe within a few decades in the second half of the past century. During the tree-health checking in the chestnut andwalnut plantation in Romania, Hargita county, next to Homoródkarácsonyfalva village, we especially concentraded on the signs of blight disease occurrence. The grove is laying on a western slope, under a pine forest. This favourable geographical space protects it not only from pathogen attacts, but it has a special, mild microclimate for nut and chestnut trees. The European chestnut could be a valuable member of local forests, opening a new perspective under conditions of climate changes.

GENETIC DIVERSITY OF EUROPEAN CHESTNUT (CASTANEA SATIVA MILL.) IN ISTRIA AND PRIMORSKO-GORANSKA COUNTY (CROATIA)

2014 ◽  
pp. 87-90
Author(s):  
I. Prgomet ◽  
D. Torello Marinoni ◽  
D. Donno ◽  
Z. Prgomet ◽  
I. Mujić ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Castanea Sativa ◽  
European Chestnut ◽  
Castanea Sativa Mill

Effects of partial in vitro digestion on properties of European chestnut ( Castanea sativa Mill) flour

2016 ◽  
Vol 640 ◽  
pp. 36-41 ◽  
Author(s):  
Bruna De Mônaco Lopes ◽  
Ivo Mottin Demiate ◽  
Vivian Cristina Ito ◽  
Cristina Soltovski de Oliveira ◽  
Marco Aurélio da Silva Carvalho Filho ◽  
...  
Keyword(s):  
Castanea Sativa ◽  
In Vitro Digestion ◽  
European Chestnut ◽  
Castanea Sativa Mill

scholarly journals First Report of CRISPR/Cas9 Gene Editing in Castanea sativa Mill

2021 ◽  
Vol 12 ◽  
Author(s):  
Vera Pavese ◽  
Andrea Moglia ◽  
Elena Corredoira ◽  
Mª Teresa Martínez ◽  
Daniela Torello Marinoni ◽  
...  
Keyword(s):  
Somatic Embryos ◽  
Gene Editing ◽  
Genome Engineering ◽  
High Efficiency ◽  
Chlorophyll Biosynthesis ◽  
Point Mutations ◽  
Castanea Sativa ◽  
Visual Assessment ◽  
European Chestnut ◽  
Selection Medium

CRISPR/Cas9 has emerged as the most important tool for genome engineering due to its simplicity, design flexibility, and high efficiency. This technology makes it possible to induce point mutations in one or some target sequences simultaneously, as well as to introduce new genetic variants by homology-directed recombination. However, this approach remains largely unexplored in forest species. In this study, we reported the first example of CRISPR/Cas9-mediated gene editing in Castanea genus. As a proof of concept, we targeted the gene encoding phytoene desaturase (pds), whose mutation disrupts chlorophyll biosynthesis allowing for the visual assessment of knockout efficiency. Globular and early torpedo-stage somatic embryos of Castanea sativa (European chestnut) were cocultured for 5 days with a CRISPR/Cas9 construct targeting two conserved gene regions of pds and subsequently cultured on a selection medium with kanamycin. After 8 weeks of subculture on selection medium, four kanamycin-resistant embryogenetic lines were isolated. Genotyping of these lines through target Sanger sequencing of amplicons revealed successful gene editing. Cotyledonary somatic embryos were maturated on maltose 3% and cold-stored at 4°C for 2 months. Subsequently, embryos were subjected to the germination process to produce albino plants. This study opens the way to the use of the CRISPR/Cas9 system in European chestnut for biotechnological applications

scholarly journals MULTI-PURPOSE CHESTNUT CLUSTERS DETECTION USING DEEP LEARNING: A PRELIMINARY APPROACH

2019 ◽  
Vol XLII-3/W8 ◽  
pp. 1-7 ◽  
Author(s):  
T. Adão ◽  
L. Pádua ◽  
T. M. Pinho ◽  
J. Hruška ◽  
A. Sousa ◽  
...  
Keyword(s):  
Phytophthora Cinnamomi ◽  
Agricultural Practices ◽  
Castanea Sativa ◽  
Cryphonectria Parasitica ◽  
Nutritional Deficiencies ◽  
Strong Increase ◽  
Environmental Disaster ◽  
Gall Wasp ◽  
European Chestnut ◽  
Portuguese Economy

<p><strong>Abstract.</strong> In the early 1980′s, the European chestnut tree (<i>Castanea sativa, Mill.</i>) assumed an important role in the Portuguese economy. Currently, the Trás-os-Montes region (Northeast of Portugal) concentrates the highest chestnuts production in Portugal, representing the major source of income in the region (€50M-€60M).</p> <p>The recognition of the quality of the Portuguese chestnut varieties has increasing the international demand for both industry and consumer-grade segments. As result, chestnut cultivation intensification has been witnessed, in such a way that widely disseminated monoculture practices are currently increasing environmental disaster risks. Depending on the dynamics of the location of interest, monocultures may lead to desertification and soil degradation even if it encompasses multiple causes and a whole range of consequences or impacts. In Trás-os-Montes, despite the strong increase in the cultivation area, phytosanitary problems, such as the chestnut ink disease (<i>Phytophthora cinnamomi</i>) and the chestnut blight (<i>Cryphonectria parasitica</i>), along with other threats, e.g. chestnut gall wasp (<i>Dryocosmus kuriphilus</i>) and nutritional deficiencies, are responsible for a significant decline of chestnut trees, with a real impact on production. The intensification of inappropriate agricultural practices also favours the onset of phytosanitary problems. Moreover, chestnut trees management and monitoring generally rely on in-field time-consuming and laborious observation campaigns. To mitigate the associated risks, it is crucial to establish an effective management and monitoring process to ensure crop cultivation sustainability, preventing at the same time risks of desertification and land degradation.</p> <p>Therefore, this study presents an automatic method that allows to perform chestnut clusters identification, a key-enabling task towards the achievement of important goals such as production estimation and multi-temporal crop evaluation. The proposed methodology consists in the use of Convolutional Neural Networks (CNNs) to classify and segment the chestnut fruits, considering a small dataset acquired based on digital terrestrial camera.</p>

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