Infection capacities in the orange-pathogen relationship: Compatible (Penicillium digitatum) and incompatible (Penicillium expansum) interactions

2012 ◽  
Vol 29 (1) ◽  
pp. 56-66 ◽  
Author(s):  
L. Vilanova ◽  
I. Viñas ◽  
R. Torres ◽  
J. Usall ◽  
A.M. Jauset ◽  
...  
Keyword(s):  
Penicillium Digitatum ◽  
Penicillium Expansum

scholarly journals A Novel Secreted Cysteine-Rich Anionic (Sca) Protein from the Citrus Postharvest Pathogen Penicillium digitatum Enhances Virulence and Modulates the Activity of the Antifungal Protein B (AfpB)

2020 ◽  
Vol 6 (4) ◽  
pp. 203
Author(s):  
Sandra Garrigues ◽  
Jose F. Marcos ◽  
Paloma Manzanares ◽  
Mónica Gandía
Keyword(s):  
Citrus Fruit ◽  
Penicillium Digitatum ◽  
Antifungal Protein ◽  
Penicillium Expansum ◽  
Wild Type ◽  
Phenotypic Differences ◽  
Antifungal Proteins ◽  
Ascomycete Fungi ◽  
In Vitro Antifungal Activity

Antifungal proteins (AFPs) from ascomycete fungi could help the development of antimycotics. However, little is known about their biological role or functional interactions with other fungal biomolecules. We previously reported that AfpB from the postharvest pathogen Penicillium digitatum cannot be detected in the parental fungus yet is abundantly produced biotechnologically. While aiming to detect AfpB, we identified a conserved and novel small Secreted Cysteine-rich Anionic (Sca) protein, encoded by the gene PDIG_23520 from P. digitatum CECT 20796. The sca gene is expressed during culture and early during citrus fruit infection. Both null mutant (Δsca) and Sca overproducer (Scaop) strains show no phenotypic differences from the wild type. Sca is not antimicrobial but potentiates P. digitatum growth when added in high amounts and enhances the in vitro antifungal activity of AfpB. The Scaop strain shows increased incidence of infection in citrus fruit, similar to the addition of purified Sca to the wild-type inoculum. Sca compensates and overcomes the protective effect of AfpB and the antifungal protein PeAfpA from the apple pathogen Penicillium expansum in fruit inoculations. Our study shows that Sca is a novel protein that enhances the growth and virulence of its parental fungus and modulates the activity of AFPs.

scholarly journals Penicillium digitatum Suppresses Production of Hydrogen Peroxide in Host Tissue During Infection of Citrus Fruit

2007 ◽  
Vol 97 (11) ◽  
pp. 1491-1500 ◽  
Author(s):  
D. Macarisin ◽  
L. Cohen ◽  
A. Eick ◽  
G. Rafael ◽  
E. Belausov ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Laser Scanning ◽  
Citrus Fruit ◽  
Laser Scanning Confocal Microscopy ◽  
Host Tissue ◽  
Penicillium Digitatum ◽  
Penicillium Expansum ◽  
Control Value ◽  
Scanning Confocal Microscopy ◽  
Production Of Hydrogen

During the infection of citrus fruit by Penicillium digitatum there is little evidence of a host defense response. This suggests that P. digitatum has the ability to suppress host defenses. The current study demonstrates that P. digitatum suppresses a defense-related hydrogen peroxide (H2O2) burst in host tissue. In contrast, the nonhost pathogen, Penicillium expansum, triggers production of a significant amount of H2O2 in citrus fruit exocarp. Using laser scanning confocal microscopy, we demonstrated that P. digitatum suppressed an elevation in H2O2 up to 42 h after inoculation. Nevertheless, H2O2 levels around wounds inoculated with P. expansum increased by 63-fold above the control. P. digitatum continued to suppress H2O2 production in citrus fruit exocarp up to 66 h postinoculation and H2O2 levels were actually threefold below that of noninoculated controls. In contrast, the H2O2 level was still about 11-fold above the control value in wound sites inoculated with P. expansum. Studies on the effect of organic acids (as pH modulators) on the response of citrus fruit to compatible and noncompatible pathogens indicated that pathogenicity was enhanced only when host-tissue acidification was accompanied by the suppression of H2O2. Additionally, pathogenicity of both P. digitatum and P. expansum on citrus fruit was significantly enhanced by the H2O2-scavenging enzyme catalase. Based on our study and previous reports regarding the potential involvement of citric acid and catalase in green mold pathogenesis, we suggest that these compounds are strongly associated with the virulence of P. digitatum.

scholarly journals Activite Antifongique Des Flavonoïdes Isoles De La Plante Asteriscus Graveolens Subsp. Odorus (Schousb.) Greuter

2016 ◽  
Vol 12 (12) ◽  
pp. 258
Author(s):  
Hakim Alilou ◽  
Bouchaib Bencharki ◽  
Jalal Talbi ◽  
Noureddine Barka
Keyword(s):  
Botrytis Cinerea ◽  
Caffeic Acid ◽  
Fruits And Vegetables ◽  
High Sensitivity ◽  
Penicillium Digitatum ◽  
Penicillium Expansum ◽  
Activité Antifongique ◽  
Post Harvest ◽  
The Subject

A phenol acid and three flavones isolated from the plant Asteriscus graveolens subsp. odorus were tested against three fungi: Botrytis cinerea, Penicillium digitatum and Penicillium expansum. Using the method of incorporation of products in a PDA, a high sensitivity of Botrytis cinerea to caffeic acid and nevadensine was observed. Penicillium digitatum is the sensitive to luteolin, as to Penicillium expansum, it marks a sensitivity to artemetin. The efficacy of the tested molecules could be the subject of an investigation and exploitation in the integrated fight against the three tested fungi that cause a lot of damage to fruits and vegetables post-harvest.

Acidification of apple and orange hosts by Penicillium digitatum and Penicillium expansum

2014 ◽  
Vol 178 ◽  
pp. 39-49 ◽  
Author(s):  
L. Vilanova ◽  
I. Viñas ◽  
R. Torres ◽  
J. Usall ◽  
G. Buron-Moles ◽  
...  
Keyword(s):  
Penicillium Digitatum ◽  
Penicillium Expansum
Sign in / Sign up

Export Citation Format

Share Document