Optimization of a rapid dot-blot immunoassay for detection of Salmonella enterica serovar Enteritidis in poultry products and environmental samples

2004 ◽  
Vol 21 (6) ◽  
pp. 761-769 ◽  
Author(s):  
Ziad W Jaradat ◽  
Joanna H Bzikot ◽  
Jerzy Zawistowski ◽  
Arun K Bhunia
Keyword(s):  
Salmonella Enterica ◽  
Environmental Samples ◽  
Dot Blot ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Poultry Products

scholarly journals Complete Genome Sequences of 14 Salmonella enterica Serovar Enteritidis Strains Recovered from Human Clinical Cases between 1949 and 1995 in the United States

2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Daniel A. Tadesse ◽  
Maria Hoffmann ◽  
Saul Sarria ◽  
Claudia Lam ◽  
Eric Brown ◽  
...  
Keyword(s):  
United States ◽  
Foodborne Pathogens ◽  
Salmonella Enterica ◽  
Complete Genome ◽  
The United States ◽  
Genome Sequences ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Poultry Products ◽  
Clinical Cases

ABSTRACT Salmonella enterica serovar Enteritidis is one of the most commonly isolated foodborne pathogens and is transmitted primarily to humans through consumption of contaminated poultry and poultry products. We are reporting completely closed genome and plasmid sequences of historical S. Enteritidis isolates recovered from humans between 1949 and 1995 in the United States.

Environmental contamination and detection of Salmonella enterica serovar enteritidis in laying flocks

2001 ◽  
Vol 149 (23) ◽  
pp. 699-704 ◽  
Author(s):  
R. Davies ◽  
M. Breslin
Keyword(s):  
Environmental Contamination ◽  
Salmonella Enterica ◽  
Environmental Samples ◽  
Egg Production ◽  
Vaccination Status ◽  
Wild Birds ◽  
Sample Type ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Free Range

Faecal, dust and other environmental samples were collected from the floors, droppings belts, egg-collection systems and other areas of 14 cage-layer flocks, 10 barn egg production flocks and seven free-range flocks, and cultured for Salmonella species. The distribution of the organism varied with its prevalence and with the vaccination status of the birds. No one sample type was found to be suitable for identifying all contaminated houses. Salmonella was also frequently found on egg-packing equipment and in samples from rodents and wild birds.

scholarly journals Oligosaccharides from Palm Kernel Cake Enhances Adherence Inhibition and Intracellular Clearance of Salmonella enterica Serovar Enteritidis In Vitro

2020 ◽  
Vol 8 (2) ◽  
pp. 255 ◽  
Author(s):  
Rui Qing Foo ◽  
Mohammad Faseleh Jahromi ◽  
Wei Li Chen ◽  
Syahida Ahmad ◽  
Kok Song Lai ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Palm Kernel ◽  
Foodborne Pathogen ◽  
Degree Of Polymerization ◽  
Palm Kernel Cake ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Poultry Products ◽  
Culture Model ◽  
A Cell

Salmonella enterica serovar (ser.) Enteritidis (S. Enteritidis) is a foodborne pathogen often associated with contaminated poultry products. This study evaluated the anti-adherence and intracellular clearance capability of oligosaccharides extracted from palm kernel cake (PKC), a by-product of the palm oil industry, and compared its efficacy with commercial prebiotics— fructooligosaccharide (FOS) and mannanoligosaccharide (MOS)—against S. Enteritidis in vitro. Based on the degree of polymerization (DP), PKC oligosaccharides were further divided into ‘Small’ (DP ≤ 6) and ‘Big’ (DP > 6) fractions. Results showed that the Small and Big PKC fractions were able to reduce (p < 0.05) S. Enteritidis adherence to Cancer coli-2 (Caco-2) cells at 0.1 mg/ mL while MOS and FOS showed significant reduction at 1.0 mg/mL and 10.0 mg/mL, respectively. In terms of S. Enteritidis clearance, oligosaccharide-treated macrophages showed better S. Enteritidis clearance over time at 50 µg/mL for Small, Big and MOS, while FOS required a concentration of 500 µg/mL for a similar effect. This data highlights that oligosaccharides from PKC, particularly those of lower DP, were more effective than MOS and FOS at reducing S. Enteritidis adherence and enhancing S. Enteritidis clearance in a cell culture model.

scholarly journals Application of Rapid Dot Blot Immunoassay for Detection of Salmonella enterica Serovar Enteritidis in Eggs, Poultry, and Other Foods

2001 ◽  
Vol 67 (1) ◽  
pp. 459-461 ◽  
Author(s):  
Mark Akira Yoshimasu ◽  
Jerzy Zawistowski
Keyword(s):  
Monoclonal Antibody ◽  
Salmonella Enterica ◽  
Dot Blot ◽  
Salmonella Enterica Serovar Enteritidis

ABSTRACT Salmonella enterica serovar Enteritidis was detected in artificially inoculated eggs within 24 h through a rapid monoclonal antibody-based dot blot immunoassay. Detection in poultry and other products required 28 h. Samples were directly enriched in homogenized egg without the need for pre- or postenrichment steps. Serovar Enteritidis was detected in the presence of other bacteria when outcompeted 1:400.

Evaluation of Whole Genome Sequencing for outbreak detection of Salmonella enterica serovar Enteritidis

2020 ◽  
Author(s):  
Ainhoa Arrieta-Gisasola ◽  
Aitor Atxaerandio Landa ◽  
Javier Garaizar ◽  
Joseba Bikandi ◽  
José Karkamo ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Salmonella Enterica ◽  
Outbreak Detection ◽  
Whole Genome ◽  
Salmonella Enterica Serovar Enteritidis

Identification of in vivo-induced genes during infection of chickens with Salmonella enterica serovar Enteritidis

2015 ◽  
Vol 100 ◽  
pp. 1-7 ◽  
Author(s):  
Shizhong Geng ◽  
Zhicheng Liu ◽  
Zhijie Lin ◽  
Paul Barrow ◽  
Zhiming Pan ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Induced Genes

scholarly journals Naturally Occurring Motility-Defective Mutants of Salmonella enterica Serovar Enteritidis Isolated Preferentially from Nonhuman Rather than Human Sources

2011 ◽  
Vol 77 (21) ◽  
pp. 7740-7748 ◽  
Author(s):  
Lucía Yim ◽  
Laura Betancor ◽  
Arací Martínez ◽  
Clare Bryant ◽  
Duncan Maskell ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Intestinal Epithelial ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Transcriptional Responses ◽  
Content Type ◽  
Field Isolates ◽  
Specific Pcr ◽  
Human Intestinal Epithelial Cells ◽  
Natural Isolates ◽  
Cell Invasiveness

ABSTRACTSalmonellosis represents a worldwide health problem because it is one of the major causes of food-borne disease. Although motility is postulated as an importantSalmonellavirulence attribute, there is little information about variation in motility in natural isolates. Here we report the identification of a point mutation (T551 → G) inmotA, a gene essential for flagellar rotation, in severalSalmonella entericaserovar Enteritidis field isolates. This mutation results in bacteria that can biosynthesize structurally normal but paralyzed flagella and are impaired in their capacity to invade human intestinal epithelial cells. Introduction of a wild-type copy ofmotAinto one of these isolates restored both motility and cell invasiveness. ThemotAmutant triggered higher proinflammatory transcriptional responses than an aflagellate isolate in differentiated Caco-2 cells, suggesting that the paralyzed flagella are able to signal through pattern recognition receptors. A specific PCR was designed to screen for the T551 → G mutation in a collection of 266S. Enteritidis field isolates from a nationwide epidemic, comprising 194 from humans and 72 from other sources. We found that 72 of the 266 (27%) isolates were nonmotile, including 24.7% (48/194) of human and 33.3% (24/72) of food isolates. Among nonmotile isolates, 15 carried the T551 → G mutation and, significantly, 13 were recovered from food, including 7 from eggs, but only 2 were from human sources. These results suggest that the presence of paralyzed flagella may impair the ability ofS. Enteritidis to cause disease in the human host but does not prevent its ability to colonize chickens and infect eggs.

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