Comparison of repetitive element sequence-based PCR with multilocus enzyme electrophoresis and pulsed field gel electrophoresis for typing Listeria monocytogenes food isolates

2004 ◽  
Vol 21 (3) ◽  
pp. 305-312 ◽  
Author(s):  
J Harvey
Keyword(s):  
Listeria Monocytogenes ◽  
Gel Electrophoresis ◽  
Repetitive Element ◽  
Pulsed Field Gel Electrophoresis ◽  
Enzyme Electrophoresis ◽  
Pulsed Field ◽  
Multilocus Enzyme Electrophoresis ◽  
Element Sequence

scholarly journals Genetic characterization ofMycobacterium aviumisolates recovered from humans and animals in Australia

1996 ◽  
Vol 116 (1) ◽  
pp. 41-49 ◽  
Author(s):  
M. M. Feizabadi ◽  
I. D. Robertson ◽  
D. V. Cousins ◽  
D. Dawson ◽  
W. Chew ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mycobacterium Avium ◽  
Gel Electrophoresis ◽  
Genetic Characterization ◽  
Genetic Relationships ◽  
Pulsed Field Gel Electrophoresis ◽  
Pfge Type ◽  
Enzyme Electrophoresis ◽  
Pulsed Field ◽  
Multilocus Enzyme Electrophoresis

SummaryGenetic relationships amongst 115 mainly Australian isolates ofMycobacterium aviumwere assessed using multilocus enzyme electrophoresis (MEE). The isolates were divided into 58 electrophoretic types (ETs), with a mean genetic diversity of 0·29. Isolates from humans were closely related to but distinct from those cultured from birds, whilst some porcine isolates belonged to the same ETs as certain human isolates. Pulsed field gel electrophoresis (PFGE) was used to differentiate related isolates, and those from birds and some from other animals, including pigs, were distinguished from the human isolates. The results of MEE and PFGE suggested that certain strains ofM. aviummay be transmitted between birds and pigs, but there was no clear evidence of transmission to humans. The serovar of theM. aviumisolates was not obviously related to their ET assignment or their PFGE type.

scholarly journals Efficacy of Pulsed-Field Gel Electrophoresis and Repetitive Element Sequence-Based PCR in Typing ofSalmonellaIsolates from Assam, India

2018 ◽  
Vol 56 (5) ◽  
Author(s):  
Purnima Gogoi ◽  
Probodh Borah ◽  
Iftikar Hussain ◽  
Leena Das ◽  
Girin Hazarika ◽  
...  
Keyword(s):  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Repetitive Element ◽  
Pulsed Field Gel Electrophoresis ◽  
Fecal Samples ◽  
Content Type ◽  
Pulsed Field ◽  
Typing Methods ◽  
Element Sequence

ABSTRACTA total of 12Salmonellaisolates belonging to different serovars,viz.,Salmonella entericaserovar Enteritidis (n= 4),Salmonella entericaserovar Weltevreden (n= 4),Salmonella entericaserovar Newport (n= 1),Salmonella entericaserovar Litchifield (n= 1), and untypeable strains (n= 2) were isolated from 332 diarrheic fecal samples collected from animals, birds, and humans. Of the two molecular typing methods applied,viz., repetitive element sequence-based PCR (REP-PCR) and pulsed-field gel electrophoresis (PFGE), PFGE could clearly differentiate the strains belonging to different serovars as well as differentiate between strains of the same serovar with respect to their source of isolation, whereas REP-PCR could not differentiate between strains of the same serovar. Thus, it can be suggested that PFGE is more useful and appropriate for molecular typing ofSalmonellaisolates during epidemiological investigations than REP-PCR.

Disparity between Multilocus Enzyme Electrophoresis, Microsatellite Markers and Pulsed-Field Gel Electrophoresis in epidemiological tracking of Candida albicans

2010 ◽  
Vol 82 (3) ◽  
pp. 265-281 ◽  
Author(s):  
Marcelo Fabiano Gomes Boriollo ◽  
Ricardo Antunes Dias ◽  
João Evangelista Fiorini ◽  
Nelma de Mello Silva Oliveira ◽  
Denise Madalena Palomari Spolidório ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Microsatellite Markers ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Enzyme Electrophoresis ◽  
Pulsed Field ◽  
Multilocus Enzyme Electrophoresis

scholarly journals Staphylococcus aureus strains of type 95. Spread of a single clone

1994 ◽  
Vol 113 (3) ◽  
pp. 463-470 ◽  
Author(s):  
V. T. Rosdahl ◽  
W. Witte ◽  
M. Musser ◽  
J. O. Jarløv
Keyword(s):  
Staphylococcus Aureus ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Enzyme Electrophoresis ◽  
Restriction Fragments ◽  
Pulsed Field ◽  
Multilocus Enzyme Electrophoresis ◽  
Single Clone ◽  
Clonal Origin ◽  
Reference Strains

SUMMARYStaphylococcus aureus strains of type 95 in Denmark have increased to a frequency of 20% of the total S. aureus population. A clonal origin and possible subdivision of these strains have been discussed. In the present investigation 35 epidemiologically unrelated S. aureus strains of type 95 as well as reference strains of other types have been analysed by other typing techniques including lectintyping, multilocus enzyme electrophoresis and pulsed-field gel electrophoresis of genomic restriction fragments. No subdivision could be achieved based on any of these methods and a clonal origin seems therefore possible.

scholarly journals Characterization of Recurrent and Sporadic Listeria monocytogenes Isolates from Raw Milk and Nondairy Foods by Pulsed-Field Gel Electrophoresis, Monocin Typing, Plasmid Profiling, and Cadmium and Antibiotic Resistance Determination

2001 ◽  
Vol 67 (2) ◽  
pp. 840-847 ◽  
Author(s):  
J. Harvey ◽  
A. Gilmour
Keyword(s):  
Listeria Monocytogenes ◽  
Gel Electrophoresis ◽  
Raw Milk ◽  
Pulsed Field Gel Electrophoresis ◽  
Enzyme Electrophoresis ◽  
Genetic Changes ◽  
Pulsed Field ◽  
Time Period ◽  
Plasmid Profiling

ABSTRACT Following previous surveys to assess the incidence ofListeria monocytogenes in raw milk and nondairy foods processed in Northern Ireland, isolates were characterized as recurrent or sporadic on the basis of multilocus enzyme electrophoresis (MEE) analysis and restriction fragment length polymorphism typing. In the present study, 45 representative recurrent and sporadic electrophoretic types (ETs) previously identified by MEE were subjected to pulsed-field gel electrophoresis (PFGE) of genomic DNA macrorestriction fragments, monocin typing, plasmid profiling, and an examination of resistance to cadmium and nine different antibiotics. Although PFGE proved to be capable of subdividing a number of recurrent and sporadic ETs, the grouping of strains arrived at by PFGE and MEE were in broad agreement, and previous conclusions regarding the designation of L. monocytogenes strains as recurrent or sporadic remained unaltered. It is considered that PFGE was able to detect minor genetic changes in recurrent ETs which occurred during the time period in which food surveys were carried out. Production of type E monocin (Types A to E were found among the 45 strains), plasmid carriage, and resistance to cadmium occurred more frequently in recurrent than in sporadic strains and may be important with regard to the ability of L. monocytogenes to persist in food and food-processing environments. Only 2 of 45 strains showed resistance to any of the nine antibiotics tested: two sporadic strains were resistant to tetracycline (MIC, 64 μg ml−1).

scholarly journals Caracterização molecular de Listeria monocytogenes oriundas de cortes cárneos bovinos e de abatedouros frigoríficos de bovinos localizados no Distrito Federal, Brasil

2016 ◽  
Vol 36 (10) ◽  
pp. 957-964 ◽  
Author(s):  
Joana M. Palma ◽  
◽  
Rodrigo C. Lisboa ◽  
Dália P. Rodrigues ◽  
André F.M. Santos ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Distrito Federal

RESUMO: Este trabalho teve como objetivo realizar a detecção de cepas de Listeria monocytogenes de cortes cárneos bovinos bem como no ambiente de abatedouros frigoríficos localizados no Distrito Federal, promover a sorotipificação pela reação em cadeia da polimerase (PCR), realizar antibiograma e submeter às cepas à eletroforese de campo pulsado (Pulsed-field gel electrophoresis - PFGE). Foram analisados um total de 125 cortes cárneos bovinos, 45 amostras de swabs de carcaças e 43 amostras de swabs em que foram detectados 13 cepas de Listeria monocytogenes, sendo 11 em cortes cárneos bovinos e 2 swabs de ambiente em um abatedouro frigorifico. Não foram isoladas cepas de swabs de carcaça. Dentre as 13 cepas de Listeria monocytogenes foram encontradas seis cepas do sorotipo 4b, cinco do sorotipo 1/2c e duas cepas do sorotipo 1/2a. Dentre as 11 cepas de L. monocytogenes encontradas em cortes cárneos bovino, uma (9,1%) cepa apresentou resistência a eritromicina, outra (9,1%) cepa a gentamicina e outra a ciprofloxacina (9,1%) e todas as cepas (100%) apresentaram resistência ao Ác. Nalidíxico. Das duas (2) cepas oriundas de ralos de abatedouro frigorífico, todas (100%) apresentaram resistência ao Ác. Nalidíxico e a sulfonamidas. A análise por eletroforese de campo pulsante (PFGE) demonstrou 13 diferentes pulsotipos, em que foram agrupados em 3 diferentes grupos clonais, que coincidentemente se correlacionavam com os 3 diferentes sorotipos encontrados sugerindo uma ampla disseminação desses perfis no Distrito Federal.

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