Identification of microRNAs from different tissues of chicken embryo and adult chicken

FEBS Letters ◽  
2006 ◽  
Vol 580 (15) ◽  
pp. 3610-3616 ◽  
Author(s):  
Hongtao Xu ◽  
Xiaobo Wang ◽  
Zhenglin Du ◽  
Ning Li
Keyword(s):  
Chicken Embryo ◽  
Adult Chicken ◽  
Different Tissues

scholarly journals A neuronal surface glycoprotein associated with the cytoskeleton.

1984 ◽  
Vol 99 (5) ◽  
pp. 1803-1813 ◽  
Author(s):  
B Ranscht ◽  
D J Moss ◽  
C Thomas
Keyword(s):  
Molecular Mass ◽  
Chicken Embryo ◽  
Polyclonal Antibodies ◽  
Neuronal Cell ◽  
Insoluble Fraction ◽  
Exchange Chromatography ◽  
Surface Glycoprotein ◽  
Adult Chicken ◽  
Neuronal Cell Adhesion Molecule ◽  
Partial Dissociation

A cytoskeleton-associated glycoprotein of 130-kilodalton molecular mass (GP 130) was purified from a nonionic detergent-insoluble fraction of 10-16-d-old chicken embryo brains. GP 130 is tightly associated with other proteins in actin-containing complexes (Moss, D.J., 1983, Eur. J. Biochem., 135:291-297); thus, pure protein preparations were obtained only after the partial dissociation of the complexes with the zwitterionic detergent, dimethyl dodecyl glycine (EMPIGEN BB), followed by ion-exchange chromatography and electrophoresis on preparative SDS polyacrylamide gels. Specific monoclonal and polyclonal antibodies were raised to GP 130 and used to examine its distribution in the developing nervous system. Experiments with these antibodies revealed that GP 130 is confined to nervous tissue and is restricted to the surface of neurons in cultures derived from both the central and peripheral nervous systems. This novel glycoprotein is immunologically unrelated to the neuronal cell adhesion molecule (N-CAM), or to vinculin, a protein of similar molecular mass which has been suggested to link actin filaments to the plasma membrane. In the developing chicken embryo brain, GP 130 is first detectable around day 8 after fertilization and increases to approximately 50% of its adult level by embryonal day 13. In contrast, no increase is observed over a similar developmental period in sciatic nerve. In the adult chicken, GP 130 is most abundant in brain and has a particularly high content in areas rich in dendrites and synapses.

Heat or cold chronic stress affects organ weights and Hsp70 levels in chicken embryos

2001 ◽  
Vol 81 (1) ◽  
pp. 83-87 ◽  
Author(s):  
P. E. N. Givisiez ◽  
M. M. da Silva ◽  
C. M. Mazzi ◽  
M. I. T. Ferro ◽  
J. A. Ferro ◽  
...  
Keyword(s):  
Cold Stress ◽  
Embryo Development ◽  
Chicken Embryo ◽  
Incubation Temperature ◽  
Weight Percent ◽  
Breast Muscle ◽  
Hsp70 Expression ◽  
Organ Weights ◽  
The Brain ◽  
Different Tissues

This study was carried out with the objective of evaluating the effect of heat (38.8°C) or cold (35.8°C) stress on chicken embryo development and tissues Hsp70 levels, after the 13th day of incubation. Embryo weight (percent egg weight), organ weight (percent embryo weight) and Hsp70 levels (ng Hsp70 mg–1 total protein) in different tissues (liver, breast muscle, heart, lungs, brain and kidney) were studied at the end of incubation. Cold stress induced a lower embryo weight and lower kidney and lungs weights, whereas heart and liver were lighter in heat-stressed embryos. An interaction between temperature and age was obtained only for Hsp70 levels in kidney and heart. Cold-stressed embryos showed higher Hsp70 levels in the brain, lungs and liver; a decrease in brain and breast muscle Hsp70 levels was seen from the 19th to 20th days in control embryos. Hsp70 levels increased with age in kidneys of control embryos and in heart of heat- and cold-stressed embryos. In conclusion, this study showed that chicken embryo organ weights are affected by incubation temperature, and that Hsp70 expression is tissue dependent (higher levels being seen in the brain) being cold-stress more effective in increasing Hsp70 levels in most studied tissues. Key words: Chicken embryo, development, Hsp70, incubation temperature

scholarly journals Localization of an endogenous lectin in chicken liver, intestine, and pancreas.

1979 ◽  
Vol 82 (2) ◽  
pp. 565-571 ◽  
Author(s):  
E C Beyer ◽  
K T Tokuyasu ◽  
S H Barondes
Keyword(s):  
Goblet Cells ◽  
Chicken Liver ◽  
Embryonic Chick ◽  
Adult Chicken ◽  
Pancreatic Acini ◽  
Adult Muscle ◽  
Endogenous Lectin ◽  
Liver Sinusoids ◽  
Different Tissues ◽  
In Cells

Extracts of adult chicken liver, pancreas, and intestine contain high levels of a lectin which appears to be identical to one previously purified from embryonic chick muscle. This lectin is virtually absent from adult muscle, but is highly concentrated in cells lining liver sinusoids, intestinal goblet cells, and the extracellular spaces surrounding pancreatic acini. These findings suggest that the lectin may play different roles in different tissues and at different times in the life of a chicken.

Cytoplasmic Tubules in Cells Infected with Laryngotracheitis Virus

1969 ◽  
Vol 27 ◽  
pp. 376-377
Author(s):  
A. M. Watrach
Keyword(s):  
Tissue Culture ◽  
Small Proportion ◽  
Chicken Embryo ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Morphologic Characteristics ◽  
Infectious Laryngotracheitis ◽  
Laryngotracheitis Virus ◽  
In Cells

During a study of the development of infectious laryngotracheitis (LT) virus in tissue culture cells, unusual tubular formations were found in the cytoplasm of a small proportion of the affected cells. It is the purpose of this report to describe the morphologic characteristics of the tubules and to discuss their possible association with the development of virus.The source and maintenance of the strain of LT virus have been described. Prior to this study, the virus was passed several times in chicken embryo kidney (CEK) tissue culture cells.

A Physiologic Regulator of Collagen-Induced Platelet Aggregation: Inhibition by Clq

1981 ◽  
Vol 45 (02) ◽  
pp. 110-115 ◽  
Author(s):  
György Csákó ◽  
Eva A Suba
Keyword(s):  
Platelet Aggregation ◽  
Human Platelet ◽  
Platelet Reactivity ◽  
High Specificity ◽  
Turbidimetric Method ◽  
Specific Manner ◽  
Aggregation Inhibition ◽  
Different Tissues

SummaryPlatelet aggregations were studied by a turbidimetric method in citrated human platelet-rich plasmas (PRP) in vitro. Human Clq inhibited the aggregations caused by collagens derived from different tissues and species. Clq was needed by weight in comparable quantities to collagen for neutralizing the aggregating effect. The dependence of the inhibitory reaction on the preincubation of platelets with Clq and the differences in the occurrence of aggregating substances in supernatants of PRP triggered with collagen in the presence or absence of Clq, confirmed that Clq exerts its effect by preventing fixation of collagen to platelets. In addition, the high specificity of the inhibitory action of Clq for collagen-induced platelet aggregation was demonstrated by results obtained for testing a variety of aggregating agents in combination with Clq and/or collagen.Since normal concentrations of Clq in the blood are in the range of inhibitory doses of Clq for collagen-induced platelet aggregations in vitro and upon activation of complement Clq is known to dissociate from Cl, it is proposed that Clq may participate in a highly specific manner in regulating platelet reactivity to collagen in vivo.

Differential Expression and Assembly Mode of Glutamine Synthetase Isoen-zymes in Different Tissues and Organs of Maize

2017 ◽  
Vol 43 (9) ◽  
pp. 1410 ◽  
Author(s):  
Xiao-Chun WANG ◽  
Hao-Ran ZHANG ◽  
Yi-Hao WEI ◽  
Xi-Ting JIA ◽  
Ming-Xin GU ◽  
...  
Keyword(s):  
Glutamine Synthetase ◽  
Differential Expression ◽  
Different Tissues

Histochemical Examination of Invertase Activities in the Growing Tip of the Plant Root

2017 ◽  
Vol 10 (1) ◽  
pp. 35-45
Author(s):  
N.F. Lunkova ◽  
N.A. Burmistrova ◽  
M.S. Krasavina
Keyword(s):  
Plant Root ◽  
Invertase Activity ◽  
Root Tip ◽  
Elongation Zone ◽  
Root Tips ◽  
Phloem Unloading ◽  
Meristem Cell ◽  
Transition To Elongation ◽  
Different Tissues

Background:A growing part of the root is one of the most active sinks for sucrose coming from source leaves through the phloem. In the root, sucrose is unloaded from conducting bundles and is distributed among the surrounding cells. To be involved in the metabolism, sucrose should disintegrate into hexoses by means of degrading enzymes.Aims:The aim of this research was to explore the possibility of the involvement of one such enzymes, invertase, in phloem unloading as well as distribution of its activity in the functionally different tissues of the plant root tips.Method:To estimate the enzyme activities in root tissues, we applied two techniques: the histochemical method using nitro blue tetrazolium. The localization of phloem unloading was studied with carboxyfluorescein, a fluorescent marker for symplastic transport.Results:Invertase activity was not detected in the apical part of the meristem. It appeared only between the basal part of this zone and the beginning of the elongation zone. There is the root phloem unloading in that area. Invertase activity increased with increasing the distance from the root tip and reached the highest values in the region of cell transition to elongation and in the elongation zone. The activities of the enzyme varied in different tissues of the same zone and sometimes in the neighboring cells of the same tissue. Biochemical determination of invertase activity was made in the maize root segments coincident to the zones of meristem, cell elongation and differentiation. The results of both methods of determination of invertase activity were in agreement.Conclusion:It was concluded that phloem unloading correlated with invertase activity, possibly because of the activation of invertase by unloaded sucrose. Invertase is one of the factors involved in the processes preparing the cells for their transition to elongation because the concentration of osmotically active hexoses increases after cleavage of sucrose, that stimulates water entry into the cells, which is necessary for elongation growth.

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