Heat or cold chronic stress affects organ weights and Hsp70 levels in chicken embryos

2001 ◽  
Vol 81 (1) ◽  
pp. 83-87 ◽  
Author(s):  
P. E. N. Givisiez ◽  
M. M. da Silva ◽  
C. M. Mazzi ◽  
M. I. T. Ferro ◽  
J. A. Ferro ◽  
...  
Keyword(s):  
Cold Stress ◽  
Embryo Development ◽  
Chicken Embryo ◽  
Incubation Temperature ◽  
Weight Percent ◽  
Breast Muscle ◽  
Hsp70 Expression ◽  
Organ Weights ◽  
The Brain ◽  
Different Tissues

This study was carried out with the objective of evaluating the effect of heat (38.8°C) or cold (35.8°C) stress on chicken embryo development and tissues Hsp70 levels, after the 13th day of incubation. Embryo weight (percent egg weight), organ weight (percent embryo weight) and Hsp70 levels (ng Hsp70 mg–1 total protein) in different tissues (liver, breast muscle, heart, lungs, brain and kidney) were studied at the end of incubation. Cold stress induced a lower embryo weight and lower kidney and lungs weights, whereas heart and liver were lighter in heat-stressed embryos. An interaction between temperature and age was obtained only for Hsp70 levels in kidney and heart. Cold-stressed embryos showed higher Hsp70 levels in the brain, lungs and liver; a decrease in brain and breast muscle Hsp70 levels was seen from the 19th to 20th days in control embryos. Hsp70 levels increased with age in kidneys of control embryos and in heart of heat- and cold-stressed embryos. In conclusion, this study showed that chicken embryo organ weights are affected by incubation temperature, and that Hsp70 expression is tissue dependent (higher levels being seen in the brain) being cold-stress more effective in increasing Hsp70 levels in most studied tissues. Key words: Chicken embryo, development, Hsp70, incubation temperature

scholarly journals HSF3 and Hsp70 Expression during Post-Hatch Cold Stress in Broiler Chickens Subjected to Embryonic Thermal Manipulation

2020 ◽  
Vol 7 (2) ◽  
pp. 49
Author(s):  
Amneh H. Tarkhan ◽  
Khaled M. M. Saleh ◽  
Mohammad Borhan Al-Zghoul
Keyword(s):  
Heat Shock ◽  
Cold Stress ◽  
Incubation Temperature ◽  
Body Weight Gain ◽  
Primary Objective ◽  
The Body ◽  
Hsp70 Expression ◽  
Hepatic Expression ◽  
Heat And Cold Stress ◽  
Thermal Manipulation

Decades of selective breeding for commercial purposes have rendered the broiler chicken (Gallus gallus domesticus) highly susceptible to heat and cold stress. A multitude of studies have documented the effects of thermal manipulation (TM) on broiler thermotolerance during periods of post-hatch heat stress, but very few have focused on the effect of TM on a broiler’s ability to withstand cold stress. Therefore, the primary objective of the current study is to determine the effects of TM on the acquisition of thermotolerance in broilers via their expression of the stress-associated 70 kilodalton heat shock protein (Hsp70) gene and heat shock factor 3 (HSF3) gene. Briefly, Hubbard broiler embryos were subject to TM by increasing the incubation temperature to 39 °C and 65% relative humidity (RH) for 18 h daily, from embryonic days (ED) 10 to 18. Broilers were then exposed to cold stress by decreasing the room temperature to 16 °C during post-hatch days 32 to 37. After thermal challenge, broilers were euthanized and hepatic and splenic tissues were collected. Our results showed that TM decreased the hatchability rate and body temperature but improved the body weight gain. TM generally decreased the hepatic expression but did not change the splenic expression of HSF3 during cold stress. In contrast, both hepatic and splenic Hsp70 expression decreased during cold stress. The results of the present study may suggest that TM significantly affects a broiler’s genetic response to cold stress.

scholarly journals Molecular Characterization and mRNA Expression of ISP2 and ISP4 in the Large Yellow Croaker (Larimichthys Crocea) Under Acute Cold Stress

2021 ◽  
Author(s):  
Baoying Qian
Keyword(s):  
Cold Stress ◽  
Mrna Expression ◽  
Ice Crystals ◽  
Control Group ◽  
Large Yellow Croaker ◽  
Amino Acid Residues ◽  
Larimichthys Crocea ◽  
Warm Temperate ◽  
The Brain ◽  
Different Tissues

Abstract Ice structure proteins (ISPs), also known as antifreeze proteins, can lower the point of freezing by inhibiting the growth of ice crystals and protect organisms from freezing temperatures. The large yellow croaker (Larimichthys crocea) is an important warm-temperate marine fish in Chinese aquaculture. Only a few ISP studies have been reported in this fish to date. In this study, the cDNA of ISP2 were cloned and characterized, and mRNA expression of ISP2 and ISP4 was assessed in different tissues of the large yellow croaker under different periods of acute cold stress (0, 6, 12, 24, 48 and 72 h, rewarming after 12 and 24 h). We found that ISP2 cDNA is 861 bases in length, encoding a protein of 168 amino acid residues. The mRNA expression of ISP2 and ISP4 in tissues of large yellow croaker under different periods of acute cold stress changed significantly. In comparison with the control group, ISP2 expression increased dramatically in the heart (1,976 fold) and intestine (26 fold) after 3 h of acute cold stress and increased 43 fold in the spleen after 6 h. ISP4 expression was up-regulated significantly in the brain (43 fold) and gill (376 fold) at 1 h acute cold stress, and increased 2,774 fold in the intestine at 3 h, 64 fold in muscle and 141 fold in the spleen after rewarming for 1 h after 12 h acute cold stress. These results indicate that ISP2 and ISP4 may play an important role in the response of large yellow croaker to acute cold stress.

scholarly journals Calcium Carbonate Nanoparticles—Toxicity and Effect of In Ovo Inoculation on Chicken Embryo Development, Broiler Performance and Bone Status

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 932
Author(s):  
Arkadiusz Matuszewski ◽  
Monika Łukasiewicz ◽  
Jan Niemiec ◽  
Maciej Kamaszewski ◽  
Sławomir Jaworski ◽  
...  
Keyword(s):  
Calcium Carbonate ◽  
Embryo Development ◽  
Bone Quality ◽  
Chicken Embryo ◽  
Broiler Chickens ◽  
Selection Procedure ◽  
High Specificity ◽  
Control Group ◽  
In Ovo

The use of intensive selection procedure in modern broiler chicken lines has led to the development of several skeletal disorders in broiler chickens. Therefore, current research is focused on methods to improve the bone quality in birds. In ovo technology, using nanoparticles with a high specificity to bones, is a potential approach. The present study aimed to evaluate the effect of in ovo inoculation (IOI) of calcium carbonate nanoparticles (CCN) on chicken embryo development, health status, bone characteristics, and on broiler production results and bone quality. After assessing in vitro cell viability, the IOI procedure was performed with an injection of 500 μg/mL CCN. The control group was not inoculated with CCN. Hatchability, weight, and selected bone and serum parameters were measured in embryos. Part of hatchlings were reared under standard conditions until 42 days, and production results, meat quality, and bone quality of broilers were determined. CCN did not show cytotoxicity to cells and chicken embryo and positively influenced bone parameters of the embryos and of broilers later (calcification) without negatively affecting the production results. Thus, the IOI of CCN could modify the molecular responses at the stage of embryogenesis, resulting in better mineralization, and could provide a sustained effect, thereby improving bone quality in adult birds.

scholarly journals Mineral Element Deposition and Gene Expression across Different Tissues of Cherry Valley Ducks

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 238
Author(s):  
Qianqian Song ◽  
Yi Zhang ◽  
Hao Bai ◽  
Li Zhong ◽  
Xiaofan Li ◽  
...  
Keyword(s):  
Significant Negative Correlation ◽  
Mineral Elements ◽  
Breast Muscle ◽  
Thigh Muscle ◽  
Mrna Levels ◽  
High Quality ◽  
Atomic Fluorescence ◽  
Breeding Efficiency ◽  
Almost All ◽  
Different Tissues

This study was conducted to investigate the deposition of several mineral elements and the mRNA levels of mineral-related genes across different tissues of cherry valley ducks. The contents of magnesium (Mg), potassium (K), zinc (Zn), and selenium (Se) in ducks’ breast muscle, thigh muscle, liver, skin, and tibia at the age of 0, 21, 35, 49, and 63 days, respectively, were measured using an atomic fluorescence spectrophotometer, while the mRNA levels of mineral-related genes were detected by qRT-PCR. The results revealed that the dynamics of Mg and K were generally similar in each tissue, with a significant positive correlation (p < 0.05). In the breast muscle, thigh muscle, and liver, the contents of almost all mineral elements reached their peak values (p < 0.05) at the age of 49 to 63 days. Interestingly, the expression of most mineral-related genes was the highest at birth (p < 0.05). In addition, there was a significant negative correlation between the expression of ATP1A1 and the deposition of K (r = −0.957, p < 0.05), and a similar result was found for the expression of ATP8 and the deposition of Zn (r = −0.905, p < 0.05). Taken together, Mg and K could be used as joint indicators for the precise breeding of the high-quality strain of cherry valley ducks, while the age of 49 to 63 days could be used as the reference for the best marketing age. In addition, ATP1A1 and ATP8 could be used as the key genes to detect K and Zn, respectively. Hence, the findings of this study can be used to improve the production and breeding efficiency of high-quality meat ducks.

Determination of ascorbic acid in the retina during chicken embryo development using high performance liquid chromatography and UV detection

2016 ◽  
Vol 8 (27) ◽  
pp. 5441-5447 ◽  
Author(s):  
Débora R. S. Lima ◽  
Marcelo Cossenza ◽  
Carlos Gustavo Garcia ◽  
Camila C. Portugal ◽  
Flávia F. de C. Marques ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Embryo Development ◽  
Chicken Embryo ◽  
High Performance ◽  
Uv Detection

A HPLC-UV method has been developed and validated for the determination of ascorbic acid in chicken embryo retina.

scholarly journals Influence of nanoparticles of platinum on chicken embryo development and brain morphology

2013 ◽  
Vol 8 (1) ◽  
pp. 251 ◽  
Author(s):  
Marta Prasek ◽  
Ewa Sawosz ◽  
Slawomir Jaworski ◽  
Marta Grodzik ◽  
Teresa Ostaszewska ◽  
...  
Keyword(s):  
Embryo Development ◽  
Chicken Embryo ◽  
Brain Morphology

Expression Patterns of Prp Gene During Chicken Embryo Development

2012 ◽  
Vol 7 (2) ◽  
pp. 199-204 ◽  
Author(s):  
Diao Xiao-Long ◽  
Wu Run ◽  
Liu Lei ◽  
Zhao Chun- Lin ◽  
Wang Xiong ◽  
...  
Keyword(s):  
Embryo Development ◽  
Chicken Embryo ◽  
Expression Patterns ◽  
Prp Gene

scholarly journals Broiler chicken thigh and breast muscle responses to cold stress during simulated transport before slaughter

2012 ◽  
Vol 91 (6) ◽  
pp. 1454-1464 ◽  
Author(s):  
S. Dadgar ◽  
T.G. Crowe ◽  
H.L. Classen ◽  
J.M. Watts ◽  
P.J. Shand
Keyword(s):  
Cold Stress ◽  
Broiler Chicken ◽  
Breast Muscle ◽  
Muscle Responses
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