Pre-validation study of alternative developmental toxicity test using mouse embryonic stem cell-derived embryoid bodies

2019 ◽  
Vol 123 ◽  
pp. 50-56 ◽  
Author(s):  
Jae-Hwan Lee ◽  
Seon Young Park ◽  
Changhwan Ahn ◽  
Cho-Won Kim ◽  
Ji-eun Kim ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Validation Study ◽  
Toxicity Test ◽  
Developmental Toxicity ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Embryoid Bodies

scholarly journals The Antimalaria Agent Artemisinin Exerts Antiangiogenic Effects in Mouse Embryonic Stem Cell-Derived Embryoid Bodies

2003 ◽  
Vol 83 (11) ◽  
pp. 1647-1655 ◽  
Author(s):  
Maria Wartenberg ◽  
Sandra Wolf ◽  
Paula Budde ◽  
Frank Grünheck ◽  
Helmut Acker ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Embryoid Bodies

A murine respiratory-inducing niche displays variable efficiency across human and mouse embryonic stem cell species

2007 ◽  
Vol 292 (5) ◽  
pp. L1241-L1247 ◽  
Author(s):  
Mark Denham ◽  
Brock J. Conley ◽  
Fredrik Olsson ◽  
Lerna Gulluyan ◽  
Timothy J. Cole ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Single Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Embryoid Bodies ◽  
Mouse Lung ◽  
Specific Marker ◽  
Tubule Formation ◽  
Human Specific

Human embryonic stemlike cells (hESCs) are pluripotent cells derived from blastocysts. Differentiating hESCs into respiratory lineages may benefit respiratory therapeutic programs. We previously demonstrated that 24% of all mouse embryonic stem cell (mESC) derivatives cocultured with embryonic day 11.5 (E11.5) mouse lung rudiments display immunoreactivity to the pneumonocyte II specific marker surfactant-associated protein C (Sftpc). Here we further investigate the effects of this inductive niche in terms of its competence to induce hESC derivative SFTPC immunoreactivity and the expression of other markers of terminal lung secretory units. When hESCs were cocultured as single cells, clumps of ∼10 cells or embryoid bodies (EBs), hESC derivatives formed pan-keratin-positive epithelial tubules at high frequency (>30% of all hESC derivatives). However, human-specific SFTPC immunoreactivity associated with tubule formation only at low frequency (<0.1% of all hESC derivatives). Human-specific SFTPD and secretoglobin family 1A member 1 ( SCGB1A1, also known as CC10) transcripts were detected by PCR after prolonged culture. Expression of other terminal lung secretory unit markers ( TITF1, SFTPA, and SFTPB) was not detected at any time point analyzed. On the other hand, hESC derivatives cultured as plated EBs in media previously demonstrated to induce Sftpc expression in isolated mouse fetal tracheal epithelium expressed all terminal lung secretory unit markers examined. mESCs and hESCs thus display fundamental differences in their response to the E11.5 mouse lung inductive niche, and these data provide an important step in the delineation of signaling mechanisms capable of efficiently inducing hESC differentiation into terminal secretory units of the lung.

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