scholarly journals Comparative biological impacts of an aerosol from carbon-heated tobacco and smoke from cigarettes on human respiratory epithelial cultures: A systems toxicology assessment

2018 ◽  
Vol 115 ◽  
pp. 109-126 ◽  
Author(s):  
Anita R. Iskandar ◽  
Florian Martin ◽  
Patrice Leroy ◽  
Walter K. Schlage ◽  
Carole Mathis ◽  
...  
Keyword(s):  
Systems Toxicology ◽  
Epithelial Cultures ◽  
Biological Impacts ◽  
Respiratory Epithelial ◽  
Toxicology Assessment

scholarly journals Systems Toxicology Assessment of the Biological Impact of a Candidate Modified Risk Tobacco Product on Human Organotypic Oral Epithelial Cultures

2016 ◽  
Vol 29 (8) ◽  
pp. 1252-1269 ◽  
Author(s):  
Filippo Zanetti ◽  
Alain Sewer ◽  
Carole Mathis ◽  
Anita R. Iskandar ◽  
Radina Kostadinova ◽  
...  
Keyword(s):  
Tobacco Product ◽  
Biological Impact ◽  
Systems Toxicology ◽  
Epithelial Cultures ◽  
Toxicology Assessment ◽  
Oral Epithelial

scholarly journals Respiratory Effects of Exposure to Aerosol From the Candidate Modified-Risk Tobacco Product THS 2.2 in an 18-Month Systems Toxicology Study With A/J Mice

2020 ◽  
Vol 178 (1) ◽  
pp. 138-158
Author(s):  
Bjoern Titz ◽  
Alain Sewer ◽  
Karsta Luettich ◽  
Ee Tsin Wong ◽  
Emmanuel Guedj ◽  
...  
Keyword(s):  
Respiratory Tract ◽  
Stress Responses ◽  
Respiratory Health ◽  
Heating System ◽  
Tobacco Product ◽  
Effective Measure ◽  
Systems Toxicology ◽  
Toxicology Study ◽  
Integrative Assessment ◽  
Toxicology Assessment

Abstract Smoking cessation is the most effective measure for reducing the risk of smoking-related diseases. However, switching to less harmful products (modified-risk tobacco products [MRTP]) can be an alternative to help reduce the risk for adult smokers who would otherwise continue to smoke. In an 18-month chronic carcinogenicity/toxicity study in A/J mice (OECD Test Guideline 453), we assessed the aerosol of Tobacco Heating System 2.2 (THS 2.2), a candidate MRTP based on the heat-not-burn principle, compared with 3R4F cigarette smoke (CS). To capture toxicity- and disease-relevant mechanisms, we complemented standard toxicology endpoints with in-depth systems toxicology analyses. In this part of our publication series, we report on integrative assessment of the apical and molecular exposure effects on the respiratory tract (nose, larynx, and lungs). Across the respiratory tract, we found changes in inflammatory response following 3R4F CS exposure (eg, antimicrobial peptide response in the nose), with both shared and distinct oxidative and xenobiotic responses. Compared with 3R4F CS, THS 2.2 aerosol exerted far fewer effects on respiratory tract histology, including adaptive tissue changes in nasal and laryngeal epithelium and inflammation and emphysematous changes in the lungs. Integrative analysis of molecular changes confirmed the substantially lower impact of THS 2.2 aerosol than 3R4F CS on toxicologically and disease-relevant molecular processes such as inflammation, oxidative stress responses, and xenobiotic metabolism. In summary, this work exemplifies how apical and molecular endpoints can be combined effectively for toxicology assessment and further supports findings on the reduced respiratory health risks of THS 2.2 aerosol.

scholarly journals In Vitro Systems Toxicology Assessment of Nonflavored e-Cigarette Liquids in Primary Lung Epithelial Cells

2017 ◽  
Vol 3 (1) ◽  
pp. 41-55 ◽  
Author(s):  
Ignacio Gonzalez-Suarez ◽  
Diego Marescotti ◽  
Florian Martin ◽  
Elena Scotti ◽  
Emmanuel Guedj ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Lung Epithelial Cells ◽  
Systems Toxicology ◽  
Lung Epithelial ◽  
In Vitro Systems ◽  
Toxicology Assessment

scholarly journals A framework for in vitro systems toxicology assessment of e-liquids

2016 ◽  
Vol 26 (6) ◽  
pp. 392-416 ◽  
Author(s):  
Anita R. Iskandar ◽  
Ignacio Gonzalez-Suarez ◽  
Shoaib Majeed ◽  
Diego Marescotti ◽  
Alain Sewer ◽  
...  
Keyword(s):  
Systems Toxicology ◽  
In Vitro Systems ◽  
Toxicology Assessment

The Novel Dry Extract BNO 1011 Stimulates Chloride Transport and Ciliary Beat Frequency in Human Respiratory Epithelial Cultures

2012 ◽  
Vol 26 (6) ◽  
pp. 439-443 ◽  
Author(s):  
James L. Kreindler ◽  
Bei Chen ◽  
Yael Kreitman ◽  
Jennifer Kofonow ◽  
Kelly M. Adams ◽  
...  
Keyword(s):  
Chloride Transport ◽  
Beat Frequency ◽  
Chloride Secretion ◽  
Herbal Remedies ◽  
Apical Surface ◽  
The Novel ◽  
Dry Extract ◽  
Epithelial Cultures ◽  
Respiratory Epithelial ◽  
Ciliary Beat

Background Herbal remedies predate written history and continue to be used more frequently than conventional pharmaceutical medications. The novel dry extract BNO 1011 is based on a combination of five herbs that is used to treat acute and chronic rhinosinusitis. We evaluated the pharmacologic effects of the novel dry extract BNO 1011 on human respiratory epithelial cultures specifically addressing electrolyte transport and cilia beat frequency (CBF). Methods Well-differentiated human bronchial epithelial cultures grown at an air–liquid interface were treated on the apical or basolateral surface with varying concentrations of dry extract BNO 1011. Changes in transepithelial sodium and chloride transport were determined in Ussing chambers under voltage-clamped conditions. Changes in CBF were determined using the Sissons-Ammons Video Analysis system (Ammons Engineering, Mt. Morris, MI). Results When applied to the apical surface, dry extract BNO 1011 activated forskolin-stimulated chloride secretion and ciliary beat in a dose-dependent fashion. Basolateral application of dry extract BNO 1011 did not alter the measured physiological properties. Conclusion Apical application of dry extract BNO 1011 stimulates both chloride secretion and CBF and therefore may augment mucociliary clearance.

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