The mechanism of Lactobacillus strains for their ability to remove fumonisins B1 and B2

2016 ◽  
Vol 97 ◽  
pp. 40-46 ◽  
Author(s):  
Hongfei Zhao ◽  
Xiao Wang ◽  
Junwen Zhang ◽  
Jun Zhang ◽  
Bolin Zhang
Keyword(s):  
Fumonisins B1 And B2

Production of Fumonisins B1 and B2 by Toxinogenics Strains of Fusarium moniliforme in Corn Cultures

1997 ◽  
Vol 25 (3) ◽  
pp. 375-377
Author(s):  
A. P. Peito ◽  
C. Abrantes ◽  
H. Batalha ◽  
A. M. Fernandes ◽  
I. Felgueiras
Keyword(s):  
Fusarium Moniliforme ◽  
Fumonisins B1 And B2

Derivation of Fumonisins B1 and B2 with 6-Aminoquinolyl N-Hydroxysuccinimidylcarbamate

1995 ◽  
Vol 43 (6) ◽  
pp. 1535-1537 ◽  
Author(s):  
Carlos Velazquez ◽  
Carolien van Bloemendal ◽  
Vicente Sanchis ◽  
Ramon Canela
Keyword(s):  
Fumonisins B1 And B2

scholarly journals Efficiency of crude corn extract clean-up on different columns in fumonisins determination

2005 ◽  
pp. 95-102 ◽  
Author(s):  
Biljana Abramovic ◽  
Sandra Jaksic ◽  
Zoran Masic
Keyword(s):  
Liquid Chromatography ◽  
Anion Exchange ◽  
Fluorescence Detection ◽  
Crude Extract ◽  
Reversed Phase ◽  
Lower Price ◽  
Strong Anion Exchange ◽  
Chromatographic Resolution ◽  
Immunoaffinity Columns ◽  
Fumonisins B1 And B2

The efficiencies of different clean-up procedures for crude corn extract from corn samples naturally contaminated by fumonisins B1 and B2 were compared. These procedures precede liquid chromatography determination with fluorescence detection. The efficiencies of immunoaffinity columns (IMA) strong anion exchange columns (SAX), as well as columns with reversed-phase C18 (RP C18) were investigated. No significant differences in the obtained results were found, regardless of the crude extract clea-nup procedure. However, the use of IMA columns for clean-up provided better chromatographic resolution, with the clean-up procedure being the simplest and the fastest. Also, because of the possibility of IMA column regeneration, it is possible to prepare ten samples on one column, so all in all, the lower price of SAX and RP C18 columns is of no great significance.

Liquid Chromatographic Determination of Fumonisins with 4-Fluoro-7-nitrobenzofurazan

1992 ◽  
Vol 75 (5) ◽  
pp. 829-833 ◽  
Author(s):  
Peter M Scott ◽  
Guillaume A Lawrence
Keyword(s):  
Limit Of Detection ◽  
Potassium Cyanide ◽  
Chromatographic Determination ◽  
Reversed Phase ◽  
Dihydrogen Phosphate ◽  
Corn Meal ◽  
Sodium Dihydrogen Phosphate ◽  
Corn Flakes ◽  
Ground Corn ◽  
Fumonisins B1 And B2

Abstract Fumonisins B1 and B2 are closely related mycotoxins produced by Fusarium moniliforme, F. proliferatum, and related species. Disadvantages of 2 fluorescence derivatizing reagents currently used for liquid chromatography (LC) are instability of the derivatives (with o-phthaldialdehyde-mercaptoethanol) and formation of 2 peaks (with fluorescamine). 4-Fluoro-7-nitrobenzofurazan (4-fluoro-7- nitrobenz-2-oxa-1,3-diazole; NBD-F) was, therefore, investigated. Although a larger molar excess of this reagent is required for fumonisins than for amino acids, the derivatives formed are moderately stable, and as little as 1 ng fumonisins B1 or B2 can be detected, with a linear response up to at least 50 ng injected. Reversed-phase LC (C18 column) was carried out with a mobile phase of methanol- 0.05M sodium dihydrogen phosphate adjusted to pH 5 (1 -1-1), which was mixed with an equal volume of acetonitrile-water (8 + 2) after 5 min. Using a modification of a strong anion exchange cleanup procedure, good recoveries (averages of 94 and 80%, respectively) of fumonisins B1 and B2 from ground corn, corn meal, and corn flakes in the 125̶ 5000 ng/g range were generally obtained; the limit of detection of the overall method was about100 ng/g. Similar results were obtained in studies with ground corn, corn meal, and corn flakes using naphthalene-2,3-dicarboxaldehyde-potassium cyanide (NDA-KCN) for derivatization; average recoveries of fumonisins B1 and B2 were 94 and 83%, respectively.

Ochratoxin A and fumonisins (B1 and B2) in maize from Balkan nephropathy endemic and non endemic areas of Croatia

1999 ◽  
Vol 15 (2) ◽  
pp. 67-80 ◽  
Author(s):  
Z Jurjevic ◽  
M Solfrizzo ◽  
B Cvjetkovic ◽  
G Avantaggiato ◽  
A Visconti
Keyword(s):  
Ochratoxin A ◽  
Balkan Nephropathy ◽  
Endemic Areas ◽  
Fumonisins B1 And B2

Natural occurrence of fumonisins B1 and B2 in maize from eight provinces of China in 2014

2017 ◽  
Vol 10 (2) ◽  
pp. 113-117 ◽  
Author(s):  
Yanxing Liu ◽  
Yan Jiang ◽  
Renjie Li ◽  
Minhao Pang ◽  
Yingchao Liu ◽  
...  
Keyword(s):  
Natural Occurrence ◽  
Fumonisins B1 And B2

scholarly journals Determination of Fumonisins B1 and B2 in Various Maize Products by a Combined SAX + C18 Column and Immunoaffinity Column

2000 ◽  
Vol 83 (1) ◽  
pp. 99-103 ◽  
Author(s):  
Tord E Möller ◽  
Håkan F Gustavsson
Keyword(s):  
Limit Of Detection ◽  
Fumonisin B1 ◽  
Immunoaffinity Column ◽  
Maize Flour ◽  
Main Column ◽  
Flour Sample ◽  
Fumonisin B2 ◽  
Fumonisins B1 And B2

Abstract Fumonisins B1 and B2 were determined in 42 samples of different maize products from the Swedish market by 2 different methods based on cleanup steps using an immunoaffinity column and a combination of SAX + C18 columns, respectively. A simple “precipitation step” was included before the samples were added to the main column(s), giving less column clogging, fewer interfering peaks, and better recoveries for the different sample matrixes. Recovery, repeatability, and results from the survey showed comparable results with the methods. The limit of detection for both methods was 5 μg/kg for fumonisin B1 and 10 μg/kg for fumonisin B2. All 7 maize chips analyzed and 6 of 8 popcorn samples contained fumonisins (B1 + B2) with averages of 180 and 115 μg/kg, respectively. All other samples except a maize flour sample contained little or no fumonisins.

scholarly journals Determination of Fumonisins B1 and B2 in Corn and Corn Flakes by Liquid Chromatography with Immunoaffinity Column Cleanup: Collaborative Study

2001 ◽  
Vol 84 (6) ◽  
pp. 1828-1838 ◽  
Author(s):  
Angelo Visconti ◽  
Michele Solfrizzo ◽  
Annalisa De Girolamo ◽  
H Bresch ◽  
P Burdaspal ◽  
...  
Keyword(s):  
Collaborative Study ◽  
Immunoaffinity Column ◽  
Relative Standard ◽  
Repeatability And Reproducibility ◽  
Double Extraction ◽  
Standard Deviations ◽  
Corn Flakes ◽  
Liquid Chromatographic ◽  
Fumonisins B1 And B2

Abstract A liquid chromatographic (LC) method for the determination of fumonisins B1 (FB1) and B2 (FB2) in corn and corn flakes was collaboratively studied by 23 laboratories, which analyzed 5 blind duplicate pairs of each matrix to establish the accuracy, repeatability, and reproducibility characteristics of the method. Fumonisin levels in the corn ranged from <0.05 (blank) to 1.41 μg/g for FB1 and from <0.05 to 0.56 μg/g for FB2, whereas in the corn flakes they ranged from <0.05 to 1.05 μg/g for FB1 and from <0.05 to 0.46 μg/g for FB2. The method involved double extraction with acetonitrile–methanol–water (25 + 25 + 50), cleanup through an immunoaffinity column, and LC determination of the fumonisins after derivatization with o-phthaldialdehyde. Relative standard deviations for the within-laboratory repeatability (RSDr) of the corn analyses ranged from 19 to 24% for FB1 and from 19 to 27% for FB2; for the corn flakes analyses, RSDr ranged from 9 to 21% for FB1 and from 8 to 22% for FB2. Relative standard deviations for the between-laboratories reproducibility (RSDR) of the corn analyses ranged from 22 to 28% for FB1 and from 22 to 30% for the FB2; for corn flakes analyses, RSDR ranged from 27 to 32% for FB1 and from 26 to 35% for FB2. Mean recoveries of FB1 and FB2 from corn spiked with FB1 at 0.80 μg/g and with FB2 at 0.40 μg/g were 76 and 72%, respectively; for corn flakes spiked at the same levels recoveries were 110 and 97% for FB1 and FB2, respectively. HORRAT ratios for the analyses of corn ranged from 1.44 to 1.53 for FB1 and from 0.96 to 1.48 for FB2, whereas for corn flakes they ranged from 1.60 to 1.82 for FB1 and from 1.39 to 1.68 for FB2.

Quantitation and Stability of Fumonisins B1 and B2 in Milk

1994 ◽  
Vol 77 (5) ◽  
pp. 1162-1167 ◽  
Author(s):  
Chris M Maragos ◽  
John L Richard
Keyword(s):  
Liquid Chromatography ◽  
Limit Of Detection ◽  
Analytical Techniques ◽  
Enzyme Linked Immunosorbent Assay ◽  
Color Development ◽  
Strong Anion Exchange ◽  
Low Sensitivity ◽  
No Sample Preparation ◽  
Fumonisins B1 And B2 ◽  
Lower Heating

Abstract The well-documented presence of fumonisin myco-toxins B1 and B2 (FB1 and FB2) in corn raises the possibility that these toxins are carried over into the milk of animals fed with contaminated feed. The presence of FB1 and FB2 in milk has not been assessed because of the lack of sensitive analytical techniques for this matrix. Two methods, liquid chromatography (LC) and enzyme-linked immunosorbent assay (ELISA), were adapted for the analysis of milk. The ELISA, produced commercially for screening corn, required no sample preparation and was reproducible but was of low sensitivity [concentration that inhibits color development by 50% (IC50), 1200-1600 ng FB1/mL]. The more sensitive LC method involves serial extraction of milk with methanol-acetone and strong anion exchange followed by derivatization with naphthalene-2,3-dicarboxaldehyde. Recoveries of 50 ng FB1 and FB2/mL from unpasteurized and un-homogenized milk were 84 and 83%, respectively (limit of detection, 5 ng/mL). Recoveries of FB1 from whole homogenized milk (76%) were slightly lower. Heating milk for 30 min at 62°C, to mimic pasteurization, did not significantly reduce FB1 or FB2 recovery, nor did storing milk for 11 days at 4°C. The LC method was applied to 165 samples of milk, only 1 of which was positive. This finding suggests that exposure of humans to FB1 and FB2 from milk is low.

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