Antimutagenic, antioxidant and free radical scavenging activity of ethyl acetate extracts from white, yellow and red onions

2004 ◽  
Vol 42 (4) ◽  
pp. 659-666 ◽  
Author(s):  
Mi-Yae Shon ◽  
Sang-Do Choi ◽  
Goon-Gjung Kahng ◽  
Sang-Hae Nam ◽  
Nak-Ju Sung
Keyword(s):  
Free Radical ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Ethyl Acetate Extracts

scholarly journals Wastes after distillation of Helichrysum italicum – biological active compounds and free radical scavenging activity

2021 ◽  
Vol 64 (2) ◽  
pp. 233-237
Author(s):  
Milena Nikolova ◽  
Ana Dobreva ◽  
Strahil Berkov
Keyword(s):  
Free Radical ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Biologically Active ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Active Compounds ◽  
Active Substances

Distillation wastewater, by-products from steam and water distillation as well as raw material used as control of flower heads of Helichrysum italicum were comparative analyzed for content of the biologically active compounds by GC/MS. Acetone exudates, methanol extracts and ethyl acetate fractions obtained after alkaline hydrolyze of the studied materials were received. The three types of extraction products as well as the distillation wastewater were examined for free radical scavenging activity by DPPH assay. Phenol, fatty- and organic acids, sterols, triterpenes, sugars and sugar alcohols were identified. Succinic acid and myo-inositol were identified as main components of distillation wastewater. Hydroxycinnamic acid, caffeic acid and 4(p)-hydroxybenzoic acid were dominant compounds of the ethyl acetate fractions. Triterpenes and fatty acids, sterols and flavonoids are among the main biologically active substances in the methanolic extracts and acetone exudates. The ethyl acetate fractions were found to possess the highest free radical scavenging activity (IC50 < 50 µg/mL). Significant differences in the activity between wastes and raw materials were not found. The results showed that the waste products after distillation of H. italicum contain important biologically active substances and the extracts with high antioxidant activity can be obtained from them.

Antioxidant Activities of Yinchenhao (Artemisiae Capillaris) Leaves

2020 ◽  
Vol 18 (4) ◽  
pp. 386-391
Author(s):  
Yonghun Kim ◽  
Ang Li ◽  
Junyu Wang ◽  
Wancong Yu ◽  
Fang Wan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Therapeutic Effects ◽  
Scavenging Activity

Yinchenhao (Artemisia capillaris Thunb.) is a pharmaceutical agent that not only shows therapeutic effects against hepatobiliary diseases but also offers various physiological benefits. This study examined the extraction rates of antioxidant extracts from Yinchenhao leaves using different solvents. The extraction rates using different solvents were as follows: ethanol (12.1 ± 0.87%) > water (7.7 ± 0.45%) > n-butanol (1.3 ± 0.16%) ethyl acetate > (1.3 ± 0.14%) > n-hexane (1.1 ± 0.15%). The n-butanol and ethyl acetate extracts showed higher 1,1-Diphenyl-2 picrylhydrazyl radical free radical scavenging activity, 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulphonate) free radical scavenging activity and ferric ion reducing antioxidant activity, while n-hexane extracts showed weak antioxidant activity. In conclusion, Yinchenhao leaf has potential as a natural antioxidant, and n-butanol and ethyl acetate may be effective extraction solvents for studying its antioxidant activities.

scholarly journals Phytochemical Screening and Free Radical Scavenging Activity of Hydroethanolic Leaf Extract of Senna sieberiana DC (Caesalpiniaceae) and its Fractions

2019 ◽  
pp. 1-6
Author(s):  
Alioune Dior Fall ◽  
Awa Ndiaye Sy ◽  
Serigne Ibra Mbacké Dieng ◽  
Abdou Sarr ◽  
Mbaye Dieng
Keyword(s):  
Free Radical ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Phytochemical Screening ◽  
Ic50 Values

Objectives: The aim of this study was to identify the phytochemical groups of hydroethanolic leaf extract of Senna sieberiana DC and its fractions and to investigate their free radical scavenging activity. Methods: S. sieberiana leaves were extracted with hydroethanolic solvent. From the hydroethanolic extract 3 fractions were obtained after a liquid/liquid fractionation (dichloromethane, ethyl acetate and water). Phytochemical screening of the leaf extract and its fractions was done using standard reactions. Free radical scavenging activity was assessed using DPPH and ABTS assays.  Results: Tannins, flavonoids, anthracenic derivatives, sterols and triterpenoids were the main phytochemical constituents of the leaf extract and fractions. The hydro-ethanolic leaf extract of Senna sieberiana, its dichloromethane, ethyl acetate and aqueous fractions and ascorbic acid had respective IC50 values of 191.6±3.82 - 495.73±8.96 - 165.8±4.85 - 50.40±2.65 - 19.53±0.13 µg/ml in ABTS assay.  In DPPH assay,  the IC50 values were  44.8 ± 1.22 - 218.93±9.01 - 32.13 ± 1.8 - 26.4±0.11- 4.66±0.07 µg/ml respectively for the leaf extract, dichloromethane, ethyl acetate and aqueous fractions and ascorbic acid. Conclusion: The hydroethanolic leaf extract of S. sieberiana had shown free radical scavenging activity. The aqueous fraction was more active among plant tested samples. 

scholarly journals Free-Radical Scavenging Activity (FRSA) of Secondary Metabolite Extracted from Indonesian Eucheuma spinosum

ALCHEMY ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 1-6
Author(s):  
Nurul Inayah ◽  
Masruri Masruri
Keyword(s):  
Free Radical ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Red Seaweed ◽  
Ic50 Values

IIndonesia is the biggest country in the production of red seaweed of Eucheuma spinosum. The red seaweed has bioactive compounds that have a potential activity such as phenolic compounds as well as carrageenan and pigments. This paper reported phytochemical analysis of E. spinosum harvested by a local farmer in Sumenep Island, East Java and free-radical scavenging activity (FRSA) derived from 2,2’-diphenyl-1-picrylhdrazyl (DPPH) for several organic solvents. For extraction, dried powder is added with 5.0 mL of various solvents following by ultra-sonication assisted extraction for 30 minutes. The extract was separated by centrifugation for phytochemical analysis and radical scavenging evaluation. The prospecting of dichloromethane, ethyl acetate, and n-hexane extracts indicated the potency for radical scavengers. Alkaloids, terpenoids, and saponins were secondary metabolites that indicated the presence in the extracts. The best IC50 value was presented by ethyl acetate extracts (384,86 ppm) with 38.78% for 50 ppm, while IC50 values of n-hexane, methanolic, dicloromethane extracts were 410.12, 677.76 and 685.08 ppm, respectively. Keywords: Eucheuma spinosum, phytochemical analysis, radical scavenging activity

scholarly journals PHYTOCHEMICAL ANALYSIS AND IN VITRO FREE RADICAL SCAVENGING ACTIVITY OF SUCCESSIVE EXTRACTS OF ALYSICARPUS VAGINALIS VAR. NUMMULARIFOLIUS (DC.) MIQ

2017 ◽  
Vol 9 (6) ◽  
pp. 231
Author(s):  
Susan Kurian ◽  
Leenamma Joseph ◽  
Josekumar V. S.
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Phytochemical Analysis ◽  
Scavenging Activity

Objective: The present study was carried to evaluate free radical scavenging activity and phytochemical analysis of successive extraction with hexane (AVH), ethyl acetate (AVE) and methanol (AVM) of Alysicarpus vaginalis var. nummularifolius (DC.) MIQ., a herb used in home remedies.Methods: The entire plant was dried and powdered, successively extracted (soxhlet apparatus) and concentrated using rotary vacuum evaporator. The extracts were quantitatively analyzed for phytochemicals like total phenolic, flavonoids, alkaloids and carbohydrates following standard methods. The free radical scavenging activity was evaluated with DPPH (1, 1-diphenyl-2-picrylhydrazyl), nitric oxide radical and hydrogen peroxide scavenging reactions.Results: The quantitative phytochemical studies of the crude extracts showed that AVM extract with highest phenolic, flavonoid, alkaloid and carbohydrate content which is followed by AVE and AVH. The IC50 values of AVH, AVE and AVM for scavenging DPPH, nitric oxide and hydrogen peroxide were 589.21±0.09 µg/ml, 254.65±0.08 µg/ml and 261.40±0.08 µg/ml; 533.81±0.09 µg/ml, 362.07±0.09 µg/ml and 456.36±0.08 µ/ml; 464.66±0.08 µg/ml, 380.29±0.08 µg/ml and 367.6±0.08 µg/ml respectively. Conclusion: The present study revealed that AVM extracts having high polyphenolic compounds when compared with AVE and AVH. The free radical scavenging reaction was greater in ethyl acetate and methanol extracts. This suggests that due to the high antioxidant reactions, these extracts can be useful for the treatment of oxidative stress–related diseases.

scholarly journals Biological activity studies of Sensevieria hyacinthoides extracts

2020 ◽  
Vol 5 (1) ◽  
pp. 15-20
Author(s):  
Mohammad Emdadul Islam ◽  
Tofail Ahmed Chowdhury ◽  
Nasim Sultana ◽  
Abul Hasnat Muhammad Solaiman ◽  
SM Anamul Arefin
Keyword(s):  
Biological Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity

Biological activity was studied of the different solvent extracts such as n-hexane, ethyl acetate, n-butanol, and methanol extracts of the rhizomes of Sensevieria hyacinthoides. The cytotoxic potential were examined by using brine shrimp lethality bioassay. Ethyl acetate extract exhibited quite potent activity in brine shrimp lethality bioassay with LC50 1.95 μg/mL, respectively. These result suggested that it might contain antitumor or cytotoxic agent. The ethyl acetate extract showed significant free radical scavenging activity with IC50 10.51 μg/mL and demonstrated excellent antibacterial activity. The free radical scavenging activity of the solvent extracts (n-hexane, ethyl acetate and n-butanol) were assayed by using DPPH method. The IC50 for Vit-c (Ascorbic acid) and ethyl acetate extract were found to be 3.91 and 10.51 μg/mL, respectively. Asian Australas. J. Biosci. Biotechnol. 2020, 5 (1), 15-20

Free Radical Scavenging Activity of Some Legumes Hulls Extract and Its Efficacy on Oil Oxidative Stability

2020 ◽  
Author(s):  
Alaa ElDein El-Beltagy ◽  
Salman Alharthi
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Protection Factor

Abstract Background Synthetic antioxidants have toxigenic effects, there is therefore growing interest in substituting them with natural antioxidants. Attention is being focused on extracting them from agricultural industry residuals to minimize costs. Legume seed hulls could be cheap sources of such natural antioxidants. Objective This study aims to unravel potential free radical scavenging activity, antioxidant activity, and total phenolic and flavonoid contents of some legumes’ hulls extracted by different solvents and evaluate their efficacy to enhance sunflower oil stability. Method Legume hulls extracted by different solvents were evaluated for their antioxidant activity coefficient (AAC), free radical scavenging activity [by 2,2-diphenyl-1- picrylhydrazyl (DPPH)], and phenolic and flavonoids contents. The protection factor and induction periods (rancimat test) of the highest activity extracts were evaluated. Results Sunflower seed hull ethyl acetate extract, lupine seed hull ethanol extract, and mung bean hull petroleum ether extract exhibited stronger DPPH scavenging activity, AAC, and protection factor values than other solvents. Ethyl acetate extracts of sunflower seed hulls showed an antioxidant and scavenging activity close (P&gt;0.05) to that detected for α-tocopherol. Conclusions Legume hulls may possess strong free radical scavenging and antioxidant activity. The analogous effect between sunflower hull extract and α-tocopherol make it a potential, cheaper substitute of α-tocopherol in food systems. Highlights The analogous effect between sunflower hull extract and α-tocopherol give it the potency to allow substitution at a concentration of 0.5% of sunflower hull ethyl acetate or 1% of lupine hull ethanol extract instead of 0.5% α-tocopherol to enhance induction periods and protection factors of sunflower oil.

A Preliminary Study on In Vitro Antioxidant and In Vivo Anti-Inflammatory Activity of Cissus quadrangularis Linn.

2021 ◽  
pp. 2619-2624
Author(s):  
Mamta Tiwari ◽  
Pushpraj S Gupta ◽  
Nisha Sharma
Keyword(s):  
Free Radical ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Inflammatory Activity ◽  
Scavenging Activity ◽  
Cissus Quadrangularis ◽  
Anti Inflammatory

Cissus quadrangularis L. is a member of succulent climber of family Vitaceae and it is also called as bone setter because of its bone healing property. The present study focused to assess the in vitro antioxidant and in vivo anti-inflammatory activity of the plant stem extracts in different solvents namely petroleum ether, chloroform, ethyl acetate and methanol. Among all these extracts; ethyl extract showed highest phenolic and flavonoid content (19.35±0.29mg GA/g, 8.68±0.06 RE/g dry wt. respectively). In DPPH free radical scavenging assay all mentioned extracts exhibited free radical scavenging activity but the highest free radical scavenging activity was showed by ethyl acetate extract in increasing dose dependent manner. On the basis of highest phenolic, flavonoid content and free radical scavenging activity ethyl acetate extract was selected for anti- inflammatory activity. The anti-inflammatory activity of Cissus quadrangularis Lin. was investigated in carageenan induced inflammation in Wistar albino rats. Two different doses 250mg/kg and 500mg/kg were administered to the animals. The results showed that both doses had significant reduction in inflammatory condition but dose 500mg/kg showed maximum inflammatory reduction on comparing with control and standard treated groups.

scholarly journals PHYTOCHEMICAL, GAS CHROMATOGRAPHY-MASS SPECTROMETRY PROFILING AND FREE RADICAL SCAVENGING ACTIVITY OF EXOCARPOS LONGIFOLIUS (SANTALACEAE) EXTRACTS

2018 ◽  
Vol 11 (10) ◽  
pp. 452
Author(s):  
Praptiwi Praptiwi ◽  
Ahmad Fathoni
Keyword(s):  
Free Radical ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Phytochemical Screening

Objective: The objectives of the study were to investigate phytochemical screening and gas chromatography-mass spectrometry (GC-MS) profiling of twigs and leaves of Exocarpos longifolius and its 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity.Methods: Phytochemical screening covering saponins, alkaloids, terpenoids, flavonoids, and tannins were carried out by standard methods. The free radical scavenging activity was performed by thin-layer chromatography - bioautography, followed by determining the IC50 values of active extracts. Chemical compounds of active extract were examined by gas chromatography/mass spectrometry.Results: The phytochemical screening revealed that all extracts have been shown to contain tannins and terpenoids, saponins were found in the methanol extract of twigs, and alkaloids were found in the ethyl acetate extracts of twigs and leaves, while flavonoids were found in the methanol extract and ethyl acetate extracts of twigs and leaves of E. longifolius. Ethyl acetate extract of twigs showed the best of IC50 value (15.65 μg/mL), while ethyl acetate extract of leaves and methanol extract of twigs have strong free radical scavenging activity which IC50 were 78.59 and 67.24 μg/mL, respectively. The GC-MS analysis of ethyl acetate extract of twigs has shown the presence of 12 identified compounds, and the main chemical compound is Stigmast-4-en-3-one (21.91%).Conclusions: The ethyl acetate extract of E. longifolius showed a very powerful free radical scavenging activity and revealed chemical compounds had been known to have biological activity. It might be used as a natural antioxidant.

scholarly journals Free Radical Scavenging Activity, Determination of Phenolic Compounds and HPLC-DAD/ESIMS Profile of Campomanesia Adamantium Leaves

2011 ◽  
Vol 6 (7) ◽  
pp. 1934578X1100600
Author(s):  
Aislan C.R.F. Pascoal ◽  
Carlos Augusto Ehrenfried ◽  
Marcos N. Eberlin ◽  
Maria Élida Alves Stefanello ◽  
Marcos José Salvador
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity

Numerous diseases are induced by free radicals via lipid peroxidation, protein peroxidation and DNA damage. It has been known that a variety of plant extracts have antioxidant activity to scavenge free radicals. Campomanesia adamantium (Myrtaceae) is a small tree with edible fruit, commonly known as “guavira” or “guabiroba-branca” that has been used in popular medicine as depurative anti-diarrhoeic, anti-inflammatory, anti-rheumatic and to liver diseases. In this study, the antiradical activities of ethanol crude extract of the leaves from C. adamantium and the ethyl acetate and butanol fractions obtained by partition, were determined using DPPH (2,2-Diphenyl-1-picrylhydrazyl radical) and ORAC-FL (Oxygen Radical Absorbance Capacity) assays. The total phenol content in the samples was estimated by Folin Ciocalteau method (FCR). In an initial evaluation the ethanolic extract and the fractions ethyl acetate and butanol have shown levels of phenolic compounds between 15- 74 mg GAE/g in FCR assay, showed DPPH free-radical scavenging activity with SC50 in the range of 7.77-13.35 μg/mL and demonstrated antioxidant capacity between 2648-3502 μmol TE/g of extract and fractions in the ORAC-FL assay. HPLC-DAD and ESIMS analysis revealed were that the extract of the leaves of C. adamantium studied appears to contain flavonoids as major constituents, including isoquercetrin and quercetin that exhibit proven antioxidant activity.

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