Pretilachlor has the potential to induce endocrine disruption, oxidative stress, apoptosis and immunotoxicity during zebrafish embryo development

2016 ◽  
Vol 42 ◽  
pp. 125-134 ◽  
Author(s):  
Jinhua Jiang ◽  
Yanhong Chen ◽  
Ruixian Yu ◽  
Xueping Zhao ◽  
Qiang Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Endocrine Disruption ◽  
Embryo Development ◽  
Zebrafish Embryo

Toxicity of polymer‐modified CuS nanoclusters on zebrafish embryo development

2021 ◽  
Author(s):  
Qiuping Zhang ◽  
Lifeng Wang ◽  
Qian Gao ◽  
Xinge Zhang ◽  
Yushuang Lin ◽  
...  
Keyword(s):  
Embryo Development ◽  
Zebrafish Embryo

scholarly journals Cellular repair mechanisms triggered by exposure to silver nanoparticles and ionic silver in embryonic zebrafish cells

2021 ◽  
Author(s):  
Ana C. Quevedo ◽  
Iseult Lynch ◽  
Eugenia Valsami-Jones
Keyword(s):  
Oxidative Stress ◽  
Silver Nanoparticles ◽  
Cell Death ◽  
Zebrafish Embryo ◽  
Genetic Damage ◽  
Cellular Repair ◽  
Embryo Cells ◽  
Repair Mechanisms ◽  
Toxicity Pathways ◽  
Dynamic Interplay

The dynamic interplay between toxicity pathways (oxidative stress, calcium disturbances, genetic damage) caused by nanoparticles and the repair mechanisms of inhibition of cell division and induction of cell death is explored in zebrafish embryo cells.

Cyclin-dependent kinase 8 is expressed both maternally and zygotically during zebrafish embryo development

2002 ◽  
Vol 1576 (1-2) ◽  
pp. 203-208 ◽  
Author(s):  
Elaine D. Brabazon ◽  
Ronan T. Bree ◽  
Micheal W. Carton ◽  
Maura Grealy ◽  
Lucy Byrnes
Keyword(s):  
Embryo Development ◽  
Zebrafish Embryo ◽  
Cyclin Dependent Kinase

P–221 Prospective randomized sibling study on gamete preparation, insemination and subsequent culture of human oocytes in a time-lapse system using media systems with and without antioxidants

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
S Mizumoto ◽  
H Watanabe ◽  
Y Nagao ◽  
K Tanaka ◽  
M Murakami ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Clinical Outcome ◽  
Elderly Patients ◽  
Embryo Development ◽  
Embryo Culture ◽  
Age Groups ◽  
Time Lapse ◽  
Single Step ◽  
Sperm Preparation ◽  
Media System

Abstract Study question Does the addition of antioxidants for gamete preparation, insemination and embryo culture lead to differences in embryo development and clinical outcome Summary answer Using an antioxidant-containing media system for sperm preparation, insemination and embryo culture imparts significantly higher good-quality blastocyst rates and improved clinical outcome in elderly patients. What is known already A previous study showed that adding combined antioxidants for sequential embryo culture in conventional incubators (interrupted culture) improves embryo viability and clinical outcome, especially for elderly patients. Here we investigated the combined effect of three antioxidants Acetyl-L-Carnitine (10 µM), N-Acetyl-L-Cysteine (10 µM), and α-Lipoic Acid (5 µM) during sperm preparation, insemination, and time-lapse culture in a single step medium on human embryo development and clinical outcome. Study design, size, duration Prospective randomized single center study including 143 couples for IVF/ICSI between August 2018 and December 2019. Inclusion required at least eight cumulus-oocyte-complexes (COCs) after retrieval. Cycles involving PGT, split IVF/ICSI, and surgically retrieved sperm were excluded. Immediately after retrieval oocytes were randomly distributed to a study or control media system with or without antioxidants (Vitrolife). Similarly, ejaculates were split and prepared with and without antioxidants. Participants/materials, setting, methods Sibling oocytes were inseminated in the respective group with accordingly prepared sperm. Single step embryo culture was conducted in medium with (Gx-TL) and without (G-TL) antioxidants in the EmbryoScope+. Embryo quality and clinical outcome were assessed in relation to maternal age (<35/>35 years). Good-quality embryos on day 3 were defined as 8- to 10-cells with even cells and low fragmentation; good-quality blastocysts as > 3BB. Clinical outcome was assessed after single vitrified blastocyst transfer (SVBT). Main results and the role of chance From 143 participants (female age, 34.7±3.2 years), a total of 2424 COCs were collected; 1180 COCs/916 metaphase-II (MII) oocytes were allocated to Gx-TL media and 1244 COCs/981 MII oocytes to G-TL media. Age-related analysis in Gx-TL compared with G-TL in relation to allocated MII oocytes revealed a trend for higher fertilization rates in Gx-TL for both age groups (<35: 72.1% vs. 66.9%; >35: 70.7% vs. 64.9%, P < 0.1). Good-quality day 3 embryo development/MII oocytes was higher, albeit not significant, in the elderly patients in Gx-TL (<35: 35.9% vs. 34.4%; >35: 31.1% vs. 27.9%). Overall day 5/6 blastocyst rate was similar for both media (<35: 48.2% vs. 49.9%; >35: 42.3% vs. 39.5%). Day 5/6 GQB rate was comparable for younger patients (<35: 23.8% for Gx-TL vs. 26.0% for G-TL) but significantly higher in Gx-TL in elderly patients (>35: 20.7% vs. 14.4%; P < 0.05). A total of 200 SVBT were performed; 99 in the Gx-TL- and 101 in the G-TL-arm. We noted almost similar implantation and ongoing pregnancy rates between Gx-TL vs G-TL in the younger (<35) age group (50.0% vs. 55.4%; 50.0% vs. 55.6%) but higher albeit not significant rates for Gx-TL in older (>35) patients (44.1% vs. 33.3%; 44.1% vs. 33.3%). Limitations, reasons for caution In almost 95% of the cycles, oocytes were inseminated by ICSI; thus results may not equally apply for cycles with IVF. The use of a closed time-lapse system may have prevented from some environmental oxidative stress. Therefore results may come out different with a similar study using standard incubation. Wider implications of the findings: Supplementation of antioxidants to media for gamete isolation and preparation, as well as subsequent single step time-lapse culture may improve GQE/B rates and clinical outcomes in certain age groups, plausibly through the reduction of oxidative stress. Further studies in selected sub-groups (severe OAT syndrome / testicular cases) may be indicated. Trial registration number UMIN000034482

scholarly journals Oxidative Stress in Mouse Sperm Impairs Embryo Development, Fetal Growth and Alters Adiposity and Glucose Regulation in Female Offspring

PLoS ONE ◽  
2014 ◽  
Vol 9 (7) ◽  
pp. e100832 ◽  
Author(s):  
Michelle Lane ◽  
Nicole O. McPherson ◽  
Tod Fullston ◽  
Marni Spillane ◽  
Lauren Sandeman ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Embryo Development ◽  
Fetal Growth ◽  
Female Offspring ◽  
Glucose Regulation ◽  
Mouse Sperm

scholarly journals Effect of Follicular Fluid Oxidative Stress on Subsequent Oocyte Quality and Embryo Development

2016 ◽  
Vol 26 (1) ◽  
pp. 1-6
Author(s):  
Özgür BİGE ◽  
Bülent GÜLEKLİ ◽  
Ahmet DEMİR ◽  
Funda GÖDE ◽  
Semra KOÇTÜRK ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Embryo Development ◽  
Follicular Fluid ◽  
Oocyte Quality

scholarly journals Oxidative Stress Alters the Profile of Transcription Factors Related to Early Development on In Vitro Produced Embryos

2017 ◽  
Vol 2017 ◽  
pp. 1-14 ◽  
Author(s):  
Roberta Ferreira Leite ◽  
Kelly Annes ◽  
Jessica Ispada ◽  
Camila Bruna de Lima ◽  
Érika Cristina dos Santos ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Transcription Factors ◽  
Embryo Development ◽  
Cell Biology ◽  
Bos Taurus ◽  
Early Embryo ◽  
Blastocyst Development ◽  
Oxidation Reduction ◽  
Number Of Cells

High oxygen levels during in vitro culture (IVC) can induce oxidative stress through accumulation of reactive oxygen species (ROS), negatively affecting embryo development. This study evaluated the effect of different O2 tensions during IVC on bovine blastocyst development and transcriptional status, considering transcription factors that play an essential role during early embryo development. For this purpose, embryos were produced in vitro by conventional protocols and cultured in two different oxygen tensions, physiological (5%) and atmospheric (20%). Expanded blastocysts were subjected to transcript quantitation analysis by RT-qPCR with Biomark™ HD System (Fluidigm, US), using 67 TaqMan assays specific for Bos taurus. Differences were observed in genes related to oxidation-reduction processes, DNA-dependent transcription factors, and factors related to important functional pathways for embryo development. Blastocyst rate was higher in the 5% O2 group and the number of cells was assessed, with the 5% O2 group having a higher number of cells. ROS concentration was evaluated, with a higher ROS presence in the 20% O2 group. Taken together, these results allow us to conclude that IVC of embryos at atmospheric O2 tension affects the expression of important transcription factors involved in multiple cell biology pathways that can affect embryo development, quality, and viability.

Effects of tebuconazole on cytochrome P450 enzymes, oxidative stress, and endocrine disruption in male rats

2018 ◽  
Vol 33 (8) ◽  
pp. 899-907 ◽  
Author(s):  
Jr-Di Yang ◽  
Shing-Hwa Liu ◽  
Mei-Hsiu Liao ◽  
Ruei-Ming Chen ◽  
Pei-Yu Liu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cytochrome P450 ◽  
Endocrine Disruption ◽  
Male Rats ◽  
Cytochrome P450 Enzymes

Imperatorin improves in vitro porcine embryo development by reducing oxidative stress and autophagy

2020 ◽  
Vol 146 ◽  
pp. 145-151 ◽  
Author(s):  
Dan Luo ◽  
Jia-bao Zhang ◽  
Yan-xia Peng ◽  
Jian-Bo Liu ◽  
Dong-xu Han ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Embryo Development ◽  
Porcine Embryo
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