Synergistic potential of fenvalerate and triadimefon on endocrine disruption and oxidative stress during rare minnow embryo development

2018 ◽  
Vol 33 (7) ◽  
pp. 759-769 ◽  
Author(s):  
Shenggan Wu ◽  
Gaojie Hu ◽  
Xueping Zhao ◽  
Qiang Wang ◽  
Jinhua Jiang
Keyword(s):  
Oxidative Stress ◽  
Endocrine Disruption ◽  
Embryo Development ◽  
Rare Minnow ◽  
And Oxidative Stress

56 Nobiletin affects gene expression profiles of the ERK1/2 pathway in bovine embryos produced invitro

2021 ◽  
Vol 33 (2) ◽  
pp. 135
Author(s):  
Y. N. Cajas ◽  
K. E. Cañón-Beltrán ◽  
C. L. V. Leal ◽  
A. Gutierrez-Adán ◽  
E. González ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Cell Cycle ◽  
Embryo Development ◽  
Expression Profiles ◽  
Calf Serum ◽  
Early Embryo ◽  
Bovine Embryos ◽  
Cell Stage ◽  
And Oxidative Stress

During embryo development the embryonic genome activation (EGA) is one of the most important events and in bovine embryos it occurs at the 8- to 16-cell stage. Invitro embryo production increases the levels of reactive oxygen species (ROS), which leads to the low quality of the produced blastocysts, possibly by affecting EGA. Nobiletin is an antioxidant that affects cell cycle regulation (Huang et al. 2016 Evid. Based. Complement. Alternat. Med. 2016, 2918796, https://doi.org/10.1155/2016/2918796). Therefore, we aimed to evaluate the effect of nobiletin supplementation, in two key periods of early embryo development, on blastocyst yield and expression of selected genes of the ERK1/2 pathway and oxidative stress on produced embryos. Invitro zygotes were cultured in synthetic oviductal fluid (SOF) with 5% fetal calf serum (control, C); C with 5 or 10µM nobiletin (MedChemExpress) (N5, N10); or C with 0.03% dimethyl sulfoxide (CDMSO; vehicle for nobiletin dilution) during the minor (21–54h post-insemination (hpi): 2- to 8-cell; MNEGA; 12 replicates) or major (54–96 hpi: 8- to 16-cell; MJEGA; 10 replicates) phase of EGA. The speed of development was considered and embryos that reached ≥8 cells at 54 hpi from MNEGA phase and ≥16 cells at 96 hpi from MJEGA phase, were selected and further cultured in control medium until Day 7. Embryos at ≥8 cell (MNEGA), ≥16 cell (MJEGA) stage, and Day 7 blastocysts from both periods were snap-frozen in liquid N2 for gene expression analysis (3 pools of 10 embryos/treatment). The expression of genes related to ERK1/2 pathway (H3–3B, H3–3A, NFE2L2) and oxidative stress (GPX1) were measured by quantitative PCR; H2AFZ and ACTB were used as housekeeping genes. Statistical analysis was assessed by one-way ANOVA. At 54 hpi, irrespective of nobiletin supplementation, no differences were found in the proportion of embryos that reached the 8-cell stage between groups in both phases (≈60%). At 96 hpi, nobiletin during MJEGA showed a higher proportion of embryos reaching the 16-cell stage than control groups (≈70% vs. ≈60%, respectively; P<0.001). Blastocyst yield for MNEGA and MJEGA was higher (P<0.001) for N5 (40.0±0.8% and 46.7±0.8%) and N10 (41.0±0.9% and 54.5±1.1%) compared with C (32.0±0.6% and 38.4±1.1%) and CDMSO (31.2±0.4% and 35.8±1.0%) groups, while N10 was higher (P<0.05) compared to N5 group in MJEGA. The expression of H3–3B and H3–3A were higher (P<0.05) in 8-cell embryos from N5 and N10 groups during MNEGA; while in 16-cell embryos, H3–3B and NFE2L2 were higher (P<0.05) only in the N10 group compared with both controls during MJEGA. GPX1 was upregulated in nobiletin-supplemented groups from both phases (8- and 16-cell embryos and blastocysts) compared with controls (P<0.05). In conclusion, nobiletin supplementation during minor or major EGA has a positive effect in pre-implantation embryo development and modifies the transcription of cell cycle and oxidative stress genes in early embryos. These benefits can be attributed to its bioactivity and indicate that it might be a tool to overcome EGA and ROS disorders in bovine invitro-produced embryos.This research was funded by MINECO-Spain AGL2015-70140-R, PID2019-111641RB-I00, RTI2018-093548-B-I00; SENESCYT-Ecuador; FAPESP-Brazil 2017/20339-3, CNPq-Brazil 304276/2018-9.

Metformin-induced endocrine disruption and oxidative stress of Oryzias latipes on two-generational condition

2019 ◽  
Vol 367 ◽  
pp. 171-181 ◽  
Author(s):  
Jin Wuk Lee ◽  
Yu-Jin Shin ◽  
Hokyun Kim ◽  
Heejung Kim ◽  
Jieun Kim ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Endocrine Disruption ◽  
Oryzias Latipes ◽  
And Oxidative Stress

Endocrine disruption and oxidative stress implications of artemether–lumefantrine combination therapy in the ovary and uterus of rats

2016 ◽  
Vol 35 (11) ◽  
pp. 1173-1182 ◽  
Author(s):  
AO Abolaji ◽  
OA Adesanoye ◽  
I Awogbindin ◽  
EO Farombi
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Combination Therapy ◽  
Endocrine Disruption ◽  
Tween 80 ◽  
Female Rats ◽  
Malarial Parasite ◽  
Glutathione S Transferase ◽  
Oestrogen Level ◽  
And Oxidative Stress

In the current study, we evaluated the endocrine disruption effect and oxidative stress implication of therapeutic dose of artemether–lumefantrine combination therapy on the ovary and uterus of rats. In this respect, female rats were divided into four groups: animals were per orally treated with tween 80 (control), artemether (4 mg kg−1 body weight), lumefantrine (24 mg kg−1 body weight) and artemether–lumefantrine (artemether, 4 mg kg−1 body weight and lumefantrine, 24 mg kg−1 body weight). We found that therapeutic doses of the drugs did not change the levels of ovarian hydrogen peroxide (H2O2) and malondialdehyde (MDA), but increased uterine levels of H2O2 and MDA and reduced ovarian and uterine levels of reduced glutathione. In addition, whilst ovarian glutathione peroxidase (GPx) activity reduced in the lumefantrine monotherapy group, uterine GPx increased in the artemether monotherapy as well as the artemether–lumefantrine groups. Furthermore, the drugs reduced ovarian and uterine glutathione- S-transferase and uterine superoxide dismutase activities. The drugs reduced oestrogen level, whereas follicle-stimulating hormone was reduced by lumefantrine and artemether–lumefantrine therapies. Additionally, artemether and lumefantrine monotherapies significantly increased prolactin and progesterone levels compared with the control ( p < 0.05). The results suggest that in the absence of malarial parasite infection, the drugs induced oxidative stress in the ovary and uterus and disrupt hormonal balance in the rats.

Endocrine disruption and oxidative stress implications of imipenem therapy in the ovary of 'wistar' rats

2018 ◽  
Author(s):  
Amal Tahri ◽  
Manel Naifar ◽  
Salima Daoud ◽  
Faten Haj Kacem ◽  
Tarek Rebai ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Endocrine Disruption ◽  
Wistar Rats ◽  
And Oxidative Stress

Development, molecular composition and freeze tolerance of bovine embryos cultured in TCM-199 supplemented with hyaluronan

Zygote ◽  
2008 ◽  
Vol 16 (1) ◽  
pp. 39-47 ◽  
Author(s):  
A.T. Palasz ◽  
P. Beltrán Breña ◽  
Marcelo F. Martinez ◽  
S.S. Perez-Garnelo ◽  
M.A. Ramirez ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Molecular Weight ◽  
Embryo Development ◽  
Culture Medium ◽  
Freeze Tolerance ◽  
Bovine Embryos ◽  
Glut 1 ◽  
Significant Difference ◽  
And Oxidative Stress

SummaryHyaluronan (HA) is glycosaminoglycan that is present from the start of embryonic development and its role and concentration increases with embryo development. The objective of this study was to evaluate if the presence of HA in TCM-199 culture medium had an effect on the development and quality of bovine embryos. There was no effect of HA on the total number of zygotes developing to blastocysts on day 7, however more expanded and hatched blastocyst stages were observed on days 8 and 9 in the group supplemented with HA (p < 0.05). Following freeze/thawing, significantly more (p < 0.05) embryos cultured in medium supplemented with HA hatched than those cultured in TCM-199 alone or those with BSA. Medium supplemented with HA and BSA significantly increased the level of expression of glucose metabolism Glut-1 gene and embryo compaction Cx43 gene (p < 0.05), and had no effect on Glut-5 and IGF-II expression. In addition, HA presence in culture decreased the level of expression of apoptosis Bax and oxidative stress SOX genes (p < 0.05). There was significant difference in total number of nuclei between TCM-199 medium only and the remaining media containing BSA or HA plus BSA, between which there was no difference. In summary, our results indicate that the addition of high molecular weight HA to TCM-199 medium that contains BSA on day 4 of culture improved embryo development to hatching and hatched blastocysts and the quality of produced embryos, which were superior to embryos cultured without HA addition.

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