Performance evaluation of Bacteroidales genetic markers for human and animal microbial source tracking in tropical agricultural watersheds

2018 ◽  
Vol 236 ◽  
pp. 100-110 ◽  
Author(s):  
Pornjira Somnark ◽  
Natcha Chyerochana ◽  
Skorn Mongkolsuk ◽  
Kwanrawee Sirikanchana
Keyword(s):  
Performance Evaluation ◽  
Genetic Markers ◽  
Microbial Source Tracking ◽  
Source Tracking ◽  
Agricultural Watersheds

Performance of host-associated genetic markers for microbial source tracking in China

2020 ◽  
Vol 175 ◽  
pp. 115670 ◽  
Author(s):  
Yang Zhang ◽  
Renren Wu ◽  
Kairong Lin ◽  
Yishu Wang ◽  
Junqing Lu
Keyword(s):  
Genetic Markers ◽  
Microbial Source Tracking ◽  
Source Tracking

scholarly journals PCR data and comparative performance of Bacteroidales microbial source tracking genetic markers

Data in Brief ◽  
2018 ◽  
Vol 19 ◽  
pp. 156-169 ◽  
Author(s):  
Pornjira Somnark ◽  
Natcha Chyerochana ◽  
Akechai Kongprajug ◽  
Skorn Mongkolsuk ◽  
Kwanrawee Sirikanchana
Keyword(s):  
Genetic Markers ◽  
Microbial Source Tracking ◽  
Source Tracking ◽  
Comparative Performance

scholarly journals Hollow-Fiber Ultrafiltration and PCR Detection of Human-Associated Genetic Markers from Various Types of Surface Water in Florida

2010 ◽  
Vol 76 (12) ◽  
pp. 4116-4117 ◽  
Author(s):  
Stephaney D. Leskinen ◽  
Miriam Brownell ◽  
Daniel V. Lim ◽  
Valerie J. Harwood
Keyword(s):  
Surface Water ◽  
Genetic Markers ◽  
Hollow Fiber ◽  
Membrane Filtration ◽  
Microbial Source Tracking ◽  
Pcr Detection ◽  
Source Tracking ◽  
Marker Genes ◽  
Water Types ◽  
Marker Detection

ABSTRACT Hollow-fiber ultrafiltration (HFUF) and PCR were combined to detect human-associated microbial source tracking marker genes in large volumes of fresh and estuarine Florida water. HFUF allowed marker detection when membrane filtration did not, demonstrating HFUF's ability to facilitate detection of diluted targets by PCR in a variety of water types.

scholarly journals Long-Term Monitoring of Waterborne Pathogens and Microbial Source Tracking Markers in Paired Agricultural Watersheds under Controlled and Conventional Tile Drainage Management

2014 ◽  
Vol 80 (12) ◽  
pp. 3708-3720 ◽  
Author(s):  
Graham Wilkes ◽  
Julie Brassard ◽  
Thomas A. Edge ◽  
Victor Gannon ◽  
Natalie Gottschall ◽  
...  
Keyword(s):  
Microbial Source Tracking ◽  
Tile Drainage ◽  
Source Tracking ◽  
Agricultural Watersheds ◽  
Viral Pathogens ◽  
Content Type ◽  
Norovirus Gii ◽  
Term Monitoring ◽  
Hobby Farm

ABSTRACTSurface waters from paired agricultural watersheds under controlled tile drainage (CTD) and uncontrolled tile drainage (UCTD) were monitored over 7 years in order to determine if there was an effect of CTD (imposed during the growing season) on occurrences and loadings of bacterial and viral pathogens, coliphages, and microbial source tracking markers. There were significantly lower occurrences of human, ruminant, and livestock (ruminant plus pig)Bacteroidalesmarkers in the CTD watershed in relation to the UCTD watershed. As for pathogens, there were significantly lower occurrences ofSalmonellaspp. andArcobacterspp. in the CTD watershed. There were no instances where there were significantly higher quantitative loadings of any microbial target in the CTD watershed, except for F-specific DNA (F-DNA) and F-RNA coliphages, perhaps as a result of fecal inputs from a hobby farm independent of the drainage practice treatments. There was lower loading of the ruminant marker in the CTD watershed in relation to the UCTD system, and results were significant at the levelP= 0.06. The odds ofSalmonellaspp. occurring increased when a ruminant marker was present relative to when the ruminant marker was absent, yet forArcobacterspp., the odds of this pathogen occurring significantly decreased when a ruminant marker was present relative to when the ruminant marker was absent (but increased when a wildlife marker was present relative to when the wildlife marker was absent). Interestingly, the odds of norovirus GII (associated with human and swine) occurring in water increased significantly when a ruminant marker was present relative to when a ruminant marker was absent. Overall, this study suggests that fecal pollution from tile-drained fields to stream could be reduced by CTD utilization.

Enterococcus and Escherichia coli fecal source apportionment with microbial source tracking genetic markers – Is it feasible?

2013 ◽  
Vol 47 (18) ◽  
pp. 6849-6861 ◽  
Author(s):  
Dan Wang ◽  
Andreas H. Farnleitner ◽  
Katharine G. Field ◽  
Hyatt C. Green ◽  
Orin C. Shanks ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Source Apportionment ◽  
Genetic Markers ◽  
Microbial Source Tracking ◽  
Source Tracking

scholarly journals Biotic Interactions and Sunlight Affect Persistence of Fecal Indicator Bacteria and Microbial Source Tracking Genetic Markers in the Upper Mississippi River

2014 ◽  
Vol 80 (13) ◽  
pp. 3952-3961 ◽  
Author(s):  
Asja Korajkic ◽  
Brian R. McMinn ◽  
Orin C. Shanks ◽  
Mano Sivaganesan ◽  
G. Shay Fout ◽  
...  
Keyword(s):  
Genetic Markers ◽  
Mississippi River ◽  
Early Stage ◽  
Fecal Indicator Bacteria ◽  
Biotic Interactions ◽  
Microbial Source Tracking ◽  
Indicator Bacteria ◽  
Source Tracking ◽  
Viral Lysis ◽  
Fecal Indicator

ABSTRACTThe sanitary quality of recreational waters that may be impacted by sewage is assessed by enumerating fecal indicator bacteria (FIB) (Escherichia coliand enterococci); these organisms are found in the gastrointestinal tracts of humans and many other animals, and hence their presence provides no information about the pollution source. Microbial source tracking (MST) methods can discriminate between different pollution sources, providing critical information to water quality managers, but relatively little is known about factors influencing the decay of FIB and MST genetic markers following release into aquatic environments. Anin situmesocosm was deployed at a temperate recreational beach in the Mississippi River to evaluate the effects of ambient sunlight and biotic interactions (predation, competition, and viral lysis) on the decay of culture-based FIB, as well as molecularly based FIB (Entero1a and GenBac3) and human-associated MST genetic markers (HF183 and HumM2) measured by quantitative real-time PCR (qPCR). In general, culturable FIB decayed the fastest, while molecularly based FIB and human-associated genetic markers decayed more slowly. There was a strong correlation between the decay of molecularly based FIB and that of human-associated genetic markers (r2, 0.96 to 0.98;P< 0.0001) but not between culturable FIB and any qPCR measurement. Overall, exposure to ambient sunlight may be an important factor in the early-stage decay dynamics but generally was not after continued exposure (i.e., after 120 h), when biotic interactions tended to be the only/major influential determinant of persistence.

Application of host-specific genetic markers for microbial source tracking of faecal water contamination in an agricultural catchment

2015 ◽  
Vol 65 (sup2) ◽  
pp. 164-172 ◽  
Author(s):  
Lisa Paruch ◽  
Adam M. Paruch ◽  
Anne-Grete Buseth Blankenberg ◽  
Marianne Bechmann ◽  
Trond Mæhlum
Keyword(s):  
Genetic Markers ◽  
Water Contamination ◽  
Microbial Source Tracking ◽  
Source Tracking
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