scholarly journals PCR data and comparative performance of Bacteroidales microbial source tracking genetic markers

Data in Brief ◽  
2018 ◽  
Vol 19 ◽  
pp. 156-169 ◽  
Author(s):  
Pornjira Somnark ◽  
Natcha Chyerochana ◽  
Akechai Kongprajug ◽  
Skorn Mongkolsuk ◽  
Kwanrawee Sirikanchana
Keyword(s):  
Genetic Markers ◽  
Microbial Source Tracking ◽  
Source Tracking ◽  
Comparative Performance

Performance of host-associated genetic markers for microbial source tracking in China

2020 ◽  
Vol 175 ◽  
pp. 115670 ◽  
Author(s):  
Yang Zhang ◽  
Renren Wu ◽  
Kairong Lin ◽  
Yishu Wang ◽  
Junqing Lu
Keyword(s):  
Genetic Markers ◽  
Microbial Source Tracking ◽  
Source Tracking

scholarly journals Hollow-Fiber Ultrafiltration and PCR Detection of Human-Associated Genetic Markers from Various Types of Surface Water in Florida

2010 ◽  
Vol 76 (12) ◽  
pp. 4116-4117 ◽  
Author(s):  
Stephaney D. Leskinen ◽  
Miriam Brownell ◽  
Daniel V. Lim ◽  
Valerie J. Harwood
Keyword(s):  
Surface Water ◽  
Genetic Markers ◽  
Hollow Fiber ◽  
Membrane Filtration ◽  
Microbial Source Tracking ◽  
Pcr Detection ◽  
Source Tracking ◽  
Marker Genes ◽  
Water Types ◽  
Marker Detection

ABSTRACT Hollow-fiber ultrafiltration (HFUF) and PCR were combined to detect human-associated microbial source tracking marker genes in large volumes of fresh and estuarine Florida water. HFUF allowed marker detection when membrane filtration did not, demonstrating HFUF's ability to facilitate detection of diluted targets by PCR in a variety of water types.

Enterococcus and Escherichia coli fecal source apportionment with microbial source tracking genetic markers – Is it feasible?

2013 ◽  
Vol 47 (18) ◽  
pp. 6849-6861 ◽  
Author(s):  
Dan Wang ◽  
Andreas H. Farnleitner ◽  
Katharine G. Field ◽  
Hyatt C. Green ◽  
Orin C. Shanks ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Source Apportionment ◽  
Genetic Markers ◽  
Microbial Source Tracking ◽  
Source Tracking

scholarly journals Biotic Interactions and Sunlight Affect Persistence of Fecal Indicator Bacteria and Microbial Source Tracking Genetic Markers in the Upper Mississippi River

2014 ◽  
Vol 80 (13) ◽  
pp. 3952-3961 ◽  
Author(s):  
Asja Korajkic ◽  
Brian R. McMinn ◽  
Orin C. Shanks ◽  
Mano Sivaganesan ◽  
G. Shay Fout ◽  
...  
Keyword(s):  
Genetic Markers ◽  
Mississippi River ◽  
Early Stage ◽  
Fecal Indicator Bacteria ◽  
Biotic Interactions ◽  
Microbial Source Tracking ◽  
Indicator Bacteria ◽  
Source Tracking ◽  
Viral Lysis ◽  
Fecal Indicator

ABSTRACTThe sanitary quality of recreational waters that may be impacted by sewage is assessed by enumerating fecal indicator bacteria (FIB) (Escherichia coliand enterococci); these organisms are found in the gastrointestinal tracts of humans and many other animals, and hence their presence provides no information about the pollution source. Microbial source tracking (MST) methods can discriminate between different pollution sources, providing critical information to water quality managers, but relatively little is known about factors influencing the decay of FIB and MST genetic markers following release into aquatic environments. Anin situmesocosm was deployed at a temperate recreational beach in the Mississippi River to evaluate the effects of ambient sunlight and biotic interactions (predation, competition, and viral lysis) on the decay of culture-based FIB, as well as molecularly based FIB (Entero1a and GenBac3) and human-associated MST genetic markers (HF183 and HumM2) measured by quantitative real-time PCR (qPCR). In general, culturable FIB decayed the fastest, while molecularly based FIB and human-associated genetic markers decayed more slowly. There was a strong correlation between the decay of molecularly based FIB and that of human-associated genetic markers (r2, 0.96 to 0.98;P< 0.0001) but not between culturable FIB and any qPCR measurement. Overall, exposure to ambient sunlight may be an important factor in the early-stage decay dynamics but generally was not after continued exposure (i.e., after 120 h), when biotic interactions tended to be the only/major influential determinant of persistence.

Application of host-specific genetic markers for microbial source tracking of faecal water contamination in an agricultural catchment

2015 ◽  
Vol 65 (sup2) ◽  
pp. 164-172 ◽  
Author(s):  
Lisa Paruch ◽  
Adam M. Paruch ◽  
Anne-Grete Buseth Blankenberg ◽  
Marianne Bechmann ◽  
Trond Mæhlum
Keyword(s):  
Genetic Markers ◽  
Water Contamination ◽  
Microbial Source Tracking ◽  
Source Tracking
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