scholarly journals Temperature depending bioelectrocatalysis current of multicopper oxidase from a hyperthermophilic archaeon Pyrobaculum aerophilum

2021 ◽  
pp. 106982
Author(s):  
Masato Tominaga ◽  
Shino Nakao ◽  
Makoto Takafuji ◽  
Eiichiro Takamura ◽  
Shin-ichiro Suye ◽  
...  
Keyword(s):  
Multicopper Oxidase ◽  
Pyrobaculum Aerophilum ◽  
Hyperthermophilic Archaeon

Construction of a biocathode using the multicopper oxidase from the hyperthermophilic archaeon, Pyrobaculum aerophilum: towards a long-life biobattery

2015 ◽  
Vol 37 (7) ◽  
pp. 1399-1404 ◽  
Author(s):  
Hiroaki Sakamoto ◽  
Toshiki Uchii ◽  
Kayo Yamaguchi ◽  
Ayako Koto ◽  
Ei-ichiro Takamura ◽  
...  
Keyword(s):  
Multicopper Oxidase ◽  
Pyrobaculum Aerophilum ◽  
Hyperthermophilic Archaeon ◽  
Long Life

A fosmid-based genomic map and identification of 474 genes of the hyperthermophilic archaeon Pyrobaculum aerophilum

Extremophiles ◽  
1997 ◽  
Vol 1 (1) ◽  
pp. 36-52 ◽  
Author(s):  
Sorel Fitz-Gibbon ◽  
Anthony J. Choi ◽  
Jeffrey H. Miller ◽  
Karl O. Stetter ◽  
Melvin I. Simon ◽  
...  
Keyword(s):  
Pyrobaculum Aerophilum ◽  
Hyperthermophilic Archaeon ◽  
Genomic Map

scholarly journals Genomic and cDNA Sequence Tags of the Hyperthermophilic Archaeon Pyrobaculum Aerophilum

1996 ◽  
Vol 24 (22) ◽  
pp. 4373-4378 ◽  
Author(s):  
P. Volkl ◽  
P. Markiewicz ◽  
C. Baikalov ◽  
S. Fitz-Gibbon ◽  
K. O. Stetter ◽  
...  
Keyword(s):  
Cdna Sequence ◽  
Pyrobaculum Aerophilum ◽  
Hyperthermophilic Archaeon

Catalytic properties and crystal structure of quinoprotein aldose sugar dehydrogenase from hyperthermophilic archaeon Pyrobaculum aerophilum

2010 ◽  
Vol 502 (2) ◽  
pp. 81-88 ◽  
Author(s):  
Haruhiko Sakuraba ◽  
Kaori Yokono ◽  
Kazunari Yoneda ◽  
Akira Watanabe ◽  
Yasuhiko Asada ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Catalytic Properties ◽  
Pyrobaculum Aerophilum ◽  
Hyperthermophilic Archaeon

scholarly journals 1P045 Crystal structure of 2-Deoxyribose-5-PhosphateAldolase from hyperthermophilic archaeon Pyrobaculum aerophilum

2005 ◽  
Vol 45 (supplement) ◽  
pp. S43
Author(s):  
K. Yoneda ◽  
K. Yoshihara ◽  
H. Sakuraba ◽  
H. Tsuge ◽  
N. Katunuma ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Pyrobaculum Aerophilum ◽  
Hyperthermophilic Archaeon

scholarly journals Properties of a Thermostable Nitrate Reductase from the Hyperthermophilic Archaeon Pyrobaculum aerophilum

2001 ◽  
Vol 183 (19) ◽  
pp. 5491-5495 ◽  
Author(s):  
Sepideh Afshar ◽  
Eric Johnson ◽  
Simon de Vries ◽  
Imke Schröder
Keyword(s):  
Nitrate Reductase ◽  
Specific Activity ◽  
Reductase Activity ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
High Specific Activity ◽  
Competitive Inhibitor ◽  
Pyrobaculum Aerophilum ◽  
Benzyl Viologen ◽  
Hyperthermophilic Archaeon

ABSTRACT The nitrate reductase of the hyperthermophilic archaeonPyrobaculum aerophilum was purified 137-fold from the cytoplasmic membrane. Based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis, the enzyme complex consists of three subunits with apparent molecular weights of 130,000, 52,000, and 32,000. The enzyme contained molybdenum (0.8-mol/mol complex), iron (15.4-mol/mol complex) and cytochrome b (0.49-mol/mol complex) as cofactors. The P. aerophilum nitrate reductase distinguishes itself from nitrate reductases of mesophilic bacteria and archaea by its very high specific activity using reduced benzyl viologen as the electron donor (V max with nitrate, 1,162 s−1 (326 U/mg);V max with chlorate, 1,348 s−1 (378 U/mg) [assayed at 75°C]). The Km values for nitrate and chlorate were 58 and 140 μM, respectively. Azide was a competitive inhibitor and cyanide was a noncompetitive inhibitor of the nitrate reductase activity. The temperature optimum for activity was >95°C. When incubated at 100°C, the purified nitrate reductase had a half-life of 1.5 h. This study constitutes the first description of a nitrate reductase from a hyperthermophilic archaeon.

The multicopper oxidase from the archaeon Pyrobaculum aerophilum shows nitrous oxide reductase activity

FEBS Journal ◽  
2010 ◽  
Vol 277 (15) ◽  
pp. 3176-3189 ◽  
Author(s):  
André T. Fernandes ◽  
João M. Damas ◽  
Smilja Todorovic ◽  
Robert Huber ◽  
M. Camilla Baratto ◽  
...  
Keyword(s):  
Nitrous Oxide ◽  
Reductase Activity ◽  
Multicopper Oxidase ◽  
Nitrous Oxide Reductase ◽  
Pyrobaculum Aerophilum

Unique active site formation in a novel galactose 1‐phosphate uridylyltransferase from the hyperthermophilic archaeon Pyrobaculum aerophilum

2020 ◽  
Vol 88 (5) ◽  
pp. 669-678 ◽  
Author(s):  
Tatsuya Ohshida ◽  
Junji Hayashi ◽  
Kazunari Yoneda ◽  
Toshihisa Ohshima ◽  
Haruhiko Sakuraba
Keyword(s):  
Active Site ◽  
Site Formation ◽  
Pyrobaculum Aerophilum ◽  
Hyperthermophilic Archaeon
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