Unique active site formation in a novel galactose 1‐phosphate uridylyltransferase from the hyperthermophilic archaeon Pyrobaculum aerophilum

2020 ◽  
Vol 88 (5) ◽  
pp. 669-678 ◽  
Author(s):  
Tatsuya Ohshida ◽  
Junji Hayashi ◽  
Kazunari Yoneda ◽  
Toshihisa Ohshima ◽  
Haruhiko Sakuraba
Keyword(s):  
Active Site ◽  
Site Formation ◽  
Pyrobaculum Aerophilum ◽  
Hyperthermophilic Archaeon

Folding Mechanism of an Ankyrin Repeat Protein: Scaffold and Active Site Formation of Human CDK Inhibitor p19INK4d

2007 ◽  
Vol 373 (1) ◽  
pp. 219-231 ◽  
Author(s):  
Christian Löw ◽  
Ulrich Weininger ◽  
Markus Zeeb ◽  
Wei Zhang ◽  
Ernest D. Laue ◽  
...  
Keyword(s):  
Active Site ◽  
Ankyrin Repeat ◽  
Site Formation ◽  
Cdk Inhibitor ◽  
Protein Scaffold ◽  
Repeat Protein ◽  
Ankyrin Repeat Protein ◽  
Folding Mechanism

A fosmid-based genomic map and identification of 474 genes of the hyperthermophilic archaeon Pyrobaculum aerophilum

Extremophiles ◽  
1997 ◽  
Vol 1 (1) ◽  
pp. 36-52 ◽  
Author(s):  
Sorel Fitz-Gibbon ◽  
Anthony J. Choi ◽  
Jeffrey H. Miller ◽  
Karl O. Stetter ◽  
Melvin I. Simon ◽  
...  
Keyword(s):  
Pyrobaculum Aerophilum ◽  
Hyperthermophilic Archaeon ◽  
Genomic Map

scholarly journals Genomic and cDNA Sequence Tags of the Hyperthermophilic Archaeon Pyrobaculum Aerophilum

1996 ◽  
Vol 24 (22) ◽  
pp. 4373-4378 ◽  
Author(s):  
P. Volkl ◽  
P. Markiewicz ◽  
C. Baikalov ◽  
S. Fitz-Gibbon ◽  
K. O. Stetter ◽  
...  
Keyword(s):  
Cdna Sequence ◽  
Pyrobaculum Aerophilum ◽  
Hyperthermophilic Archaeon

Single-molecule studies of DNA and RNA four-way junctions

2004 ◽  
Vol 32 (1) ◽  
pp. 41-45 ◽  
Author(s):  
S.A. McKinney ◽  
E. Tan ◽  
T.J. Wilson ◽  
M.K. Nahas ◽  
A.-C. Déclais ◽  
...  
Keyword(s):  
Single Molecule ◽  
Active Site ◽  
Dynamic Structure ◽  
Holliday Junctions ◽  
Site Formation ◽  
Structural Element ◽  
Fluorescence Studies ◽  
Definitive Evidence ◽  
Dna And Rna ◽  
Single Molecule Studies

Branched helical junctions are common in nucleic acids. In DNA, the four-way junction (Holliday junction) is an essential intermediate in homologous recombination and is a highly dynamic structure, capable of stacking conformer transitions and branch migration. Our single-molecule fluorescence studies provide unique insight into the energy landscape of Holliday junctions by visualizing these processes directly. In the hairpin ribozyme, an RNA four-way junction is an important structural element that enhances active-site formation by several orders of magnitude. Our single-molecule studies suggest a plausible mechanism for how the junction achieves this remarkable feat; the structural dynamics of the four-way junction bring about frequent contacts between the loops that are needed to form the active site. The most definitive evidence for this is the observation of three-state folding in single-hairpin ribozymes, the intermediate state of which is populated due to the intrinsic properties of the junction.

Catalytic properties and crystal structure of quinoprotein aldose sugar dehydrogenase from hyperthermophilic archaeon Pyrobaculum aerophilum

2010 ◽  
Vol 502 (2) ◽  
pp. 81-88 ◽  
Author(s):  
Haruhiko Sakuraba ◽  
Kaori Yokono ◽  
Kazunari Yoneda ◽  
Akira Watanabe ◽  
Yasuhiko Asada ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Catalytic Properties ◽  
Pyrobaculum Aerophilum ◽  
Hyperthermophilic Archaeon
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