Direct uptake of electrode electrons for autotrophic denitrification by Thiobacillus denitrificans

2015 ◽  
Vol 60 ◽  
pp. 126-130 ◽  
Author(s):  
Linpeng Yu ◽  
Yong Yuan ◽  
Shanshan Chen ◽  
Li Zhuang ◽  
Shungui Zhou
Keyword(s):  
Thiobacillus Denitrificans ◽  
Autotrophic Denitrification

scholarly journals Light-driven nitrous oxide production via autotrophic denitrification by self-photosensitized Thiobacillus denitrificans

2019 ◽  
Vol 127 ◽  
pp. 353-360 ◽  
Author(s):  
Man Chen ◽  
Xiao-Fang Zhou ◽  
Yu-Qing Yu ◽  
Xing Liu ◽  
Raymond Jian-Xiong Zeng ◽  
...  
Keyword(s):  
Nitrous Oxide ◽  
Thiobacillus Denitrificans ◽  
Autotrophic Denitrification ◽  
Oxide Production ◽  
Nitrous Oxide Production

Characteristics of nitrogen removal and microbial distribution by application of spent sulfidic caustic in pilot scale wastewater treatment plant

2010 ◽  
Vol 62 (6) ◽  
pp. 1440-1447 ◽  
Author(s):  
S. Park ◽  
J. Lee ◽  
J. Park ◽  
I. Byun ◽  
T. Park ◽  
...  
Keyword(s):  
Electron Donor ◽  
Nitrogen Removal ◽  
Distribution Ratio ◽  
Pilot Scale ◽  
Nitrifying Bacteria ◽  
Relative Distribution ◽  
Thiobacillus Denitrificans ◽  
Autotrophic Denitrification ◽  
Spent Sulfidic Caustic ◽  
Sulfidic Caustic

Since spent sulfidic caustic (SSC) produced from petrochemical industry contains a high concentration of alkalinity and sulfide, it was expected that SSC could be used as an electron donor for autotrophic denitrification. To investigate the nitrogen removal performance, a pilot scale Bardenpho process was operated. The total nitrogen removal efficiency increased as SSC dosage increased, and the highest efficiency was observed as 77.5% when SSC was injected into both anoxic tank (1) and (2). FISH analysis was also performed to shed light on the effect of SSC dosage on the distribution ratio of nitrifying bacteria and Thiobacillus denitrificans. FISH results indicated that the relative distribution ratio of ammonia-oxidizing bacteria, Nitrobacter spp., Nitrospira genus and Thiobacillus denitrificans to eubacteria varied little with the pH of the tanks, and SSC injection did not give harmful effect on nitrification efficiency. These results show that SSC can be applied as an electron donor of autotrophic denitrification to biological nitrogen removal process effectively, without any inhibitory effects to nitrifying bacteria and sulfur-utilizing denitrifying bacteria.

Removal of high NO3Ð concentrations in saline water through autotrophic denitrification by the bacterium Thiobacillus denitrificans strain MP

2004 ◽  
Vol 49 (5-6) ◽  
pp. 105-112 ◽  
Author(s):  
J.-D. Gu ◽  
W. Qiu ◽  
A. Koenig ◽  
Y. Fan
Keyword(s):  
Bacterial Community ◽  
Hydraulic Retention Time ◽  
Saline Water ◽  
Optimal Level ◽  
Thiobacillus Denitrificans ◽  
Autotrophic Denitrification ◽  
Efficient Removal ◽  
Column Reactor ◽  
Column System ◽  
Autotrophic Bacteria

Autotrophic denitrification by Thiobacillus denitrificans MP isolated from mangrove was investigated in both a sulphur-limestone column reactor and a fermenter. More than 97.5% of the nitrate (NO3-) in the 250 mg NO3--N/L strong influent was removed after 14.3 hours in the column reactor. Influent NO3- was completely depleted in the lower part of the column as the hydraulic retention time increased and a slight pH drop was also observed along the reactor column due to the exhaustion of the buffering ability of the limestone. Trace amounts of oxygen present in the lower part of the reactor column resulted in the accumulation of nitrite and subsequent inhibition of further denitrification. The species composition of the bacterial community in the higher parts of the reactor column was morphologically more diverse than in the lower part. Denitrification by T. denitrificans MP reached an optimal level when the dissolved oxygen was maintained between 1.5-2% of saturation level in the automated fermenter. The stoichiometric ratios of ΔSO42- produced/ΔNO3--N removed were 6.81 and 9.32 in the reactor column and fermenter, respectively. This study suggests that efficient removal of high NO3Ð concentrations in water or wastewater can be achieved using autotrophic bacteria immobilized on surfaces of sulphur granules in the column system.

Denitrification of industrial wastewater with sulfur and limestone packed column

2002 ◽  
Vol 46 (11-12) ◽  
pp. 99-104 ◽  
Author(s):  
R. Nugroho ◽  
H. Takanashi ◽  
M. Hirata ◽  
T. Hano
Keyword(s):  
Industrial Wastewater ◽  
Dominant Species ◽  
Elemental Sulfur ◽  
Packed Column ◽  
Steel Production ◽  
Thiobacillus Denitrificans ◽  
Autotrophic Denitrification ◽  
Treatment Performance ◽  
Biofilm Process ◽  
Kinetics Of

An autotrophic denitrification system was developed for nitrate contaminated industrial wastewater whose C/N ratio was very low. The microbes containing Thiobacillus denitrificans as a dominant species were attached on the surface of granular elemental sulfur packed in a column. Elemental sulfur was used as an electron donor for autotrophic denitrification. The granules of limestone were mixed with the granular sulfur to moderate the decrease of alkalinity during autotrophic denitrification. The stoichiometry and basic kinetics of denitrification were studied in column runs. The effects of minerals such as phosphorus on treatment performance were clarified. The wastewater from steel production plants was treated by the present biofilm process. Low extent of nitrogen removal was caused by the lack of minerals.

scholarly journals DEVELOPMENT OF AUTOTROPHIC DENITRIFICATION FOR NITRATE-CONTAMINATED INDUSTRIAL WASTEWATER

2018 ◽  
Vol 3 (2) ◽  
Author(s):  
Rudi Nugroho
Keyword(s):  
Rate Constant ◽  
Industrial Wastewater ◽  
Packed Column ◽  
Order Reaction ◽  
Synthetic Wastewater ◽  
Nitrate Content ◽  
Thiobacillus Denitrificans ◽  
Autotrophic Denitrification ◽  
Biofilm Thickness ◽  
Denitrification Kinetics

An autotrophic denitrification system was developed for treatment wastewater from steel industry. The aim was to evaluate the kinetics and capability of the system in reducing nitrate content in such industrial wastewater. The experiments were conducted in batch suspension and continuous runs. The denitrification kinetics in suspension runs obeys first order reaction with the rate constant k1 and k2 were determined to be 0.014 and 0.004 g-N/m3.d, respectively. The continuous runs used a column packed with mixture of granular sulfur and limestone. The microbes of Thiobacillus denitrificans were attached on the surface of granular sulfur in the form of biofilm. The biofilm thickness was investigated to be approximately 40 μm. The denitrification kinetics in the packed column obeys half-order reaction with the rate constant k of 0.172 g-N1/2.m1/2/(kg-S.d). The lower denitrification extent of industrial wastewater compared with the synthetic wastewater might be due to the lack of alkalinity. Keywords: Autotrophic denitrification, sulfur, packed column, thiobacillus denitrificans.

A Kinetic Model for Autotrophic Denitrification using Sulphur:Limestone Reactors

2003 ◽  
Vol 38 (1) ◽  
pp. 183-192 ◽  
Author(s):  
Ashref Darbi ◽  
Thiruvenkatachari Viraraghavan
Keyword(s):  
Particle Size ◽  
Kinetic Model ◽  
Reaction Rate ◽  
Average Particle Size ◽  
Order Reaction ◽  
Average Particle ◽  
Thiobacillus Denitrificans ◽  
Autotrophic Denitrification ◽  
Reaction Rate Constants ◽  
Kinetics Of

Abstract The kinetics of autotrophic denitrification of groundwater by Thiobacillus denitrificans in a sulfur:limestone upflow reactor was examined in order to predict effluent concentrations. Experiments were performed using water containing 60 and 90 mg NO3—N/L and sulfur and limestone with average particle size of 3.5 mm. Results clearly showed that nitrate was completely removed from 60 and 90 mg NO3—N/L influent concentrations. The results showed that the autotrophic denitrification rates in sulfur:limestone reactors can be described by half-order kinetics. The half-order reaction rate constants for the entire media were estimated at 1.34 and 1.54 mg1/2/L1/2 h for influent concentrations of 60 and 90 mg NO3—N/L, respectively.

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