Removal of high NO3Ð concentrations in saline water through autotrophic denitrification by the bacterium Thiobacillus denitrificans strain MP

2004 ◽  
Vol 49 (5-6) ◽  
pp. 105-112 ◽  
Author(s):  
J.-D. Gu ◽  
W. Qiu ◽  
A. Koenig ◽  
Y. Fan
Keyword(s):  
Bacterial Community ◽  
Hydraulic Retention Time ◽  
Saline Water ◽  
Optimal Level ◽  
Thiobacillus Denitrificans ◽  
Autotrophic Denitrification ◽  
Efficient Removal ◽  
Column Reactor ◽  
Column System ◽  
Autotrophic Bacteria

Autotrophic denitrification by Thiobacillus denitrificans MP isolated from mangrove was investigated in both a sulphur-limestone column reactor and a fermenter. More than 97.5% of the nitrate (NO3-) in the 250 mg NO3--N/L strong influent was removed after 14.3 hours in the column reactor. Influent NO3- was completely depleted in the lower part of the column as the hydraulic retention time increased and a slight pH drop was also observed along the reactor column due to the exhaustion of the buffering ability of the limestone. Trace amounts of oxygen present in the lower part of the reactor column resulted in the accumulation of nitrite and subsequent inhibition of further denitrification. The species composition of the bacterial community in the higher parts of the reactor column was morphologically more diverse than in the lower part. Denitrification by T. denitrificans MP reached an optimal level when the dissolved oxygen was maintained between 1.5-2% of saturation level in the automated fermenter. The stoichiometric ratios of ΔSO42- produced/ΔNO3--N removed were 6.81 and 9.32 in the reactor column and fermenter, respectively. This study suggests that efficient removal of high NO3Ð concentrations in water or wastewater can be achieved using autotrophic bacteria immobilized on surfaces of sulphur granules in the column system.

Efficient Removal of Metolachlor and Bacterial Community of Biofilm in Bioelectrochemical Reactors

2019 ◽  
Vol 189 (2) ◽  
pp. 384-395 ◽  
Author(s):  
Xiaojing Li ◽  
Xiaolin Zhang ◽  
Xiaodong Zhao ◽  
Binbin Yu ◽  
Liping Weng ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Efficient Removal

scholarly journals Light-driven nitrous oxide production via autotrophic denitrification by self-photosensitized Thiobacillus denitrificans

2019 ◽  
Vol 127 ◽  
pp. 353-360 ◽  
Author(s):  
Man Chen ◽  
Xiao-Fang Zhou ◽  
Yu-Qing Yu ◽  
Xing Liu ◽  
Raymond Jian-Xiong Zeng ◽  
...  
Keyword(s):  
Nitrous Oxide ◽  
Thiobacillus Denitrificans ◽  
Autotrophic Denitrification ◽  
Oxide Production ◽  
Nitrous Oxide Production

scholarly journals Identification of a Thiomicrospira denitrificans-Like Epsilonproteobacterium as a Catalyst for Autotrophic Denitrification in the Central Baltic Sea

2006 ◽  
Vol 72 (2) ◽  
pp. 1364-1372 ◽  
Author(s):  
Ingrid Brettar ◽  
Matthias Labrenz ◽  
Sébastien Flavier ◽  
Julia Bötel ◽  
Harri Kuosa ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Baltic Sea ◽  
Environmental Dna ◽  
Rrna Gene ◽  
Autotrophic Denitrification ◽  
Reduced Sulfur Compounds ◽  
Single Strand Conformational Polymorphism ◽  
Thiomicrospira Denitrificans ◽  
Stimulation Experiments ◽  
The Baltic

ABSTRACT Identification and functional analysis of key members of bacterial communities in marine and estuarine environments are major challenges for obtaining a mechanistic understanding of biogeochemical processes. In the Baltic Sea basins, as in many other marine environments with anoxic bodies of water, the oxic-anoxic interface is considered a layer of high bacterial turnover of sulfur, nitrogen, and carbon compounds that has a great impact on matter balances in the whole ecosystem. We focused on autotrophic denitrification by oxidation of reduced sulfur compounds as a biogeochemically important process mediating concomitant turnover of sulfur, nitrogen, and carbon. We used a newly developed approach consisting of molecular analyses in stimulation experiments and in situ abundance. The molecular approach was based on single-strand conformational polymorphism (SSCP) analysis of the bacterial community RNA, which allowed identification of potential denitrifiers based on the sequences of enhanced SSCP bands and monitoring of the overall bacterial community during the experiments. Sequences of the SSCP bands of interest were used to design highly specific primers that enabled (i) generation of almost complete 16S rRNA gene sequences using experimental and environmental DNA as templates and (ii) quantification of the bacteria of interest by real-time PCR. By using this approach we identified the bacteria responsible for autotrophic denitrification as a single taxon, an epsilonproteobacterium related to the autotrophic denitrifier Thiomicrospira denitrificans. This finding was confirmed by material balances in the experiments that were consistent with those obtained with continuous cultures of T. denitrificans. The presence and activity of a bacterium that is phylogenetically and physiologically closely related to T. denitrificans could be relevant for the carbon budget of the central Baltic Sea because T. denitrificans exhibits only one-half the efficiency for carbon dioxide fixation per mol of sulfide oxidized and mol of nitrate reduced of Thiobacillus denitrificans hypothesized previously for this function.

Autotrophic denitrification of landfill leachate using elemental sulphur

1996 ◽  
Vol 34 (5-6) ◽  
pp. 469-476 ◽  
Author(s):  
A. Koenig ◽  
L. H. Liu
Keyword(s):  
Particle Size ◽  
Retention Time ◽  
Hydraulic Retention Time ◽  
Loading Rate ◽  
Nitrate Removal ◽  
Packed Bed ◽  
Elemental Sulphur ◽  
Limiting Factor ◽  
Autotrophic Denitrification ◽  
Sulphur Particle

One of the most economical means of nitrogen removal from leachate is biological treatment by nitrification, followed by heterotrophic denitrification. An alternative biological denitrification process is autotrophic denitrification using Thiobacillus denitrificans. This autotrophic bacteria oxidizes elemental sulphur to sulphate while reducing nitrate to elemental nitrogen gas, thereby eliminating the need for addition of organic carbon compounds. For this study, pilot-scale elemental sulphur packed bed columns with fixed-film denitrification have been selected as the most suitable treatment process. The effect of hydraulic retention time as well as the effect of concentration and loading rate of nitrate on nitrate removal efficiency as a function of sulphur particle size were determined. The results indicate that (i) autotrophic denitrification can effectively remove nitrate from synthetic and actual nitrified leachate at concentrations much higher than hitherto reported; (ii) the minimum hydraulic retention time necessary for complete denitrification depends on sulphur particle size; (iii) the maximum area loading rate, in g NO3−-N/m2·d, appears to be the process limiting factor and is practically independent of sulphur particle size; and (iv) the observed stoichiometric relationships compare well with those previously reported.

scholarly journals Simultaneous removal of Fe3+ and nitrate in the autotrophic denitrification immobilized systems

2017 ◽  
Vol 18 (5) ◽  
pp. 1625-1634
Author(s):  
Jun feng Su ◽  
Ting ting Lian ◽  
Ting lin Huang ◽  
Dong hui Liang ◽  
Wen dong Wang
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Retention Time ◽  
Hydraulic Retention Time ◽  
Removal Rate ◽  
Nitrate Removal ◽  
Simultaneous Removal ◽  
Autotrophic Denitrification ◽  
Four Levels ◽  
Experimental Group

Abstract In this study, strain CC76, identified as Enterobacter sp., was tested for the reduction of Fe3+ and denitrification using immobilized pellets with strain CC76 as experimental group (IP) and immobilized pellets with strain CC76 and magnetite powder as experimental group (IPM) in the autotrophic denitrification immobilized systems (ADIS). Compared with IP, a higher nitrate removal rate was obtained with IPM by using three levels of influent Fe3+ (0, 5, and 10 mg/L), four levels of pH (5.0, 6.0, 7.0, and 8.0), and three levels of hydraulic retention time (HRT) (12, 14, and 16 h), respectively. Furthermore, response surface methodology (RSM) analysis demonstrated that the optimum removal ratios of nitrate of 87.21% (IP) and 96.27% (IPM) were observed under the following conditions: HRT of 12 h, pH of 7.0 and influent Fe3+ concentration of 5 mg/L (IP) and 1 mg/L (IPM).

Characteristics of nitrogen removal and microbial distribution by application of spent sulfidic caustic in pilot scale wastewater treatment plant

2010 ◽  
Vol 62 (6) ◽  
pp. 1440-1447 ◽  
Author(s):  
S. Park ◽  
J. Lee ◽  
J. Park ◽  
I. Byun ◽  
T. Park ◽  
...  
Keyword(s):  
Electron Donor ◽  
Nitrogen Removal ◽  
Distribution Ratio ◽  
Pilot Scale ◽  
Nitrifying Bacteria ◽  
Relative Distribution ◽  
Thiobacillus Denitrificans ◽  
Autotrophic Denitrification ◽  
Spent Sulfidic Caustic ◽  
Sulfidic Caustic

Since spent sulfidic caustic (SSC) produced from petrochemical industry contains a high concentration of alkalinity and sulfide, it was expected that SSC could be used as an electron donor for autotrophic denitrification. To investigate the nitrogen removal performance, a pilot scale Bardenpho process was operated. The total nitrogen removal efficiency increased as SSC dosage increased, and the highest efficiency was observed as 77.5% when SSC was injected into both anoxic tank (1) and (2). FISH analysis was also performed to shed light on the effect of SSC dosage on the distribution ratio of nitrifying bacteria and Thiobacillus denitrificans. FISH results indicated that the relative distribution ratio of ammonia-oxidizing bacteria, Nitrobacter spp., Nitrospira genus and Thiobacillus denitrificans to eubacteria varied little with the pH of the tanks, and SSC injection did not give harmful effect on nitrification efficiency. These results show that SSC can be applied as an electron donor of autotrophic denitrification to biological nitrogen removal process effectively, without any inhibitory effects to nitrifying bacteria and sulfur-utilizing denitrifying bacteria.

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