Systemic delivery of therapeutic small interfering RNA using a pH-triggered amphiphilic poly-l-lysine nanocarrier to suppress prostate cancer growth in mice

2012 ◽  
Vol 45 (5) ◽  
pp. 521-532 ◽  
Author(s):  
Jianfeng Guo ◽  
Woei Ping Cheng ◽  
Jingxia Gu ◽  
Caixia Ding ◽  
Xiaozhong Qu ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Small Interfering Rna ◽  
Cancer Growth ◽  
Systemic Delivery ◽  
Interfering Rna

Inhibition of PARP1 by small interfering RNA enhances docetaxel activity against human prostate cancer PC3 cells

2013 ◽  
Vol 442 (1-2) ◽  
pp. 127-132 ◽  
Author(s):  
Wenqi Wu ◽  
Zhenzhen Kong ◽  
Xiaolu Duan ◽  
Hanliang Zhu ◽  
Shujue Li ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Small Interfering Rna ◽  
Human Prostate ◽  
Human Prostate Cancer ◽  
Pc3 Cells ◽  
Interfering Rna

scholarly journals Down-regulation of Human DAB2IP Gene Expression Mediated by Polycomb Ezh2 Complex and Histone Deacetylase in Prostate Cancer

2005 ◽  
Vol 280 (23) ◽  
pp. 22437-22444 ◽  
Author(s):  
Hong Chen ◽  
Szu-wei Tu ◽  
Jer-Tsong Hsieh
Keyword(s):  
Gene Expression ◽  
Prostate Cancer ◽  
Epithelial Cells ◽  
Histone Deacetylase ◽  
Promoter Region ◽  
Small Interfering Rna ◽  
Gene Promoter ◽  
Down Regulation ◽  
Ezh2 Expression ◽  
Interfering Rna

Human DAB2IP (hDAB2IP), a novel GTPase-activating protein modulating the Ras-mediated signaling and tumor necrosis factor-mediated apoptosis, is a potent growth inhibitor in human prostate cancer (PCa). Loss of hDAB2IP expression in PCa is due to altered epigenetic regulation (i.e. DNA methylation and histone modification) of its promoter region. The elevated polycomb Ezh2, a histone methyltransferase, has been associated with PCa progression. In this study, we have demonstrated that an increased Ezh2 expression in normal prostatic epithelial cells can suppress hDAB2IP gene expression. In contrast, knocking down the endogenous Ezh2 levels in PCa by a specific small interfering RNA can increase hDAB2IP expression. The association of Ezh2 complex (including Eed and Suz12) with hDAB2IP gene promoter is also detected in PCa cells but not in normal prostatic epithelial cells. Increased Ezh2 expression in normal prostatic epithelial cells by cDNA transfection facilitates the recruitment of other components of Ezh2 complex to the hDAB2IP promoter region accompanied with the increased levels of methyl histone H3 (H3) and histone deacetylase (HDAC1). Consistently, data from PCa cells transfected with Ezh2 small interfering RNA demonstrated that reduced Ezh2 levels resulted in the dissociation of Ezh2 complex accompanied with decreased levels of both methyl H3 and HDAC1 from hDAB2IP gene promoter. We further unveiled that the methylation status of Lys-27 but not Lys-9 of H3 in hDAB2IP promoter region is consistent with the hDAB2IP levels in both normal prostatic epithelial cells and PCa cells. Together, we conclude that hDAB2IP gene is a target gene of Ezh2 in prostatic epithelium, which provides an underlying mechanism of the down-regulation of hDAB2IP gene in PCa.

scholarly journals Small-interfering RNA–induced androgen receptor silencing leads to apoptotic cell death in prostate cancer

2005 ◽  
Vol 4 (4) ◽  
pp. 505-515 ◽  
Author(s):  
Xinbo Liao ◽  
Siqing Tang ◽  
J. Brantley Thrasher ◽  
Tomas L. Griebling ◽  
Benyi Li
Keyword(s):  
Prostate Cancer ◽  
Cell Death ◽  
Androgen Receptor ◽  
Small Interfering Rna ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Interfering Rna
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