T-cadinol and calamenene induce dendritic cells from human monocytes and drive Th1 polarization

2006 ◽  
Vol 537 (1-3) ◽  
pp. 190-199 ◽  
Author(s):  
Masao Takei ◽  
Akemi Umeyama ◽  
Shigenobu Arihara
Keyword(s):  
Dendritic Cells ◽  
Human Monocytes ◽  
Th1 Polarization

scholarly journals Effect of serotonin on the differentiation of human monocytes into dendritic cells

2006 ◽  
Vol 146 (2) ◽  
pp. 354-361 ◽  
Author(s):  
N. Katoh ◽  
F. Soga ◽  
T. Nara ◽  
R. Tamagawa-Mineoka ◽  
M. Nin ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Human Monocytes

scholarly journals Role of the cytokine environment and cytokine receptor expression on the generation of functionally distinct dendritic cells from human monocytes

2008 ◽  
Vol 38 (3) ◽  
pp. 750-762 ◽  
Author(s):  
Lucia Conti ◽  
Marco Cardone ◽  
Barbara Varano ◽  
Patrizia Puddu ◽  
Filippo Belardelli ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Receptor Expression ◽  
Cytokine Receptor ◽  
Human Monocytes

scholarly journals Aggregation of the High-Affinity IgE Receptor FcεRI on Human Monocytes and Dendritic Cells Induces NF-κB Activation

2002 ◽  
Vol 118 (5) ◽  
pp. 830-837 ◽  
Author(s):  
Stefan Kraft ◽  
Natalija Novak ◽  
Thomas Bieber ◽  
Norito Katoh ◽  
Rudolf A. Rupec
Keyword(s):  
Dendritic Cells ◽  
Human Monocytes ◽  
Ige Receptor ◽  
High Affinity ◽  
High Affinity Ige Receptor

scholarly journals The Influence of Ouabain on Human Dendritic Cells Maturation

2014 ◽  
Vol 2014 ◽  
pp. 1-15 ◽  
Author(s):  
C. R. Nascimento ◽  
R. C. Valente ◽  
J. Echevarria-Lima ◽  
C. F. L. Fontes ◽  
L. de Araujo-Martins ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Lymphocyte Activation ◽  
Human Monocytes ◽  
Atpase Inhibitor ◽  
Gm Csf ◽  
Hla Dr ◽  
Distinct Category ◽  
Thymocyte Apoptosis ◽  
Human Dendritic Cells ◽  
Dc Maturation

Although known as a Na,K-ATPase inhibitor, several other cellular and systemic actions have been ascribed to the steroid Ouabain (Oua). Particularly in the immune system, our group showed that Ouabain acts on decreasing lymphocyte proliferation, synergizing with glucocorticoids in spontaneous thymocyte apoptosis, and also lessening CD14 expression and blocking CD16 upregulation on human monocytes. However, Ouabain effects on dendritic cells (DCs) were not explored so far. Considering the peculiar plasticity and the importance of DCs in immune responses, the aim of our study was to investigate DC maturation under Ouabain influence. To generate immature DCs, human monocytes were cultured with IL-4 and GM-CSF (5 days). To investigate Ouabain role on DC activation, DCs were stimulated with TNF-αfor 48 h in the presence or absence of Ouabain. TNF-induced CD83 expression and IL-12 production were abolished in DCs incubated with 100 nM Ouabain, though DC functional capacity concerning lymphocyte activation remained unaltered. Nevertheless, TNF-α-induced antigen capture downregulation, another maturation marker, occurred even in the presence of Ouabain. Besides, Ouabain increased HLA-DR and CD86 expression, whereas CD80 expression was maintained. Collectively, our results suggest that DCs respond to Ouabain maturating into a distinct category, possibly contributing to the balance between immunity and tolerance.

scholarly journals CTLA4 is expressed on mature dendritic cells derived from human monocytes and influences their maturation and antigen presentation

2011 ◽  
Vol 12 (1) ◽  
Author(s):  
Xiong B Wang ◽  
Zhong Z Fan ◽  
Doina Anton ◽  
Annika V Vollenhoven ◽  
Zhen H Ni ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Antigen Presentation ◽  
Human Monocytes

scholarly journals Recombinant ubiquitin-conjugating enzyme of Eimeria maxima induces immunogenic maturation in chicken splenic-derived dendritic cells and drives Th1 polarization in-vitro

2020 ◽  
Vol 143 ◽  
pp. 104162
Author(s):  
Shakeel Ahmed Lakho ◽  
Muhammad Haseeb ◽  
Jianmei Huang ◽  
Muhammad Waqqas Hasan ◽  
Muhammad Ali-ul-Husnain Naqvi ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Eimeria Maxima ◽  
Ubiquitin Conjugating Enzyme ◽  
Th1 Polarization ◽  
Conjugating Enzyme

Differentiation of Human Monocytes into CD14 Negative Accessory Cells: Do Dendritic Cells Derive from the Monocytic Lineage?

Pathobiology ◽  
1991 ◽  
Vol 59 (3) ◽  
pp. 122-126 ◽  
Author(s):  
J.H. Peters ◽  
J. Ruppert ◽  
R.K.H. Gieseler ◽  
H.M. Najar ◽  
H. Xu
Keyword(s):  
Dendritic Cells ◽  
Human Monocytes ◽  
Accessory Cells ◽  
Monocytic Lineage

scholarly journals Different Efficiency of Heat Shock Proteins (HSP) to Activate Human Monocytes and Dendritic Cells: Superiority of HSP60

2002 ◽  
Vol 169 (11) ◽  
pp. 6141-6148 ◽  
Author(s):  
Karen Bethke ◽  
Frank Staib ◽  
Martin Distler ◽  
Ute Schmitt ◽  
Helmut Jonuleit ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Human Monocytes ◽  
Shock Proteins

scholarly journals Glyceraldehyde-3-phosphate dehydrogenase from Eimeria acervulina modulates the functions of chicken dendritic cells to boost Th1 type immune response and stimulates autologous CD4+ T cells differentiation in-vitro

2020 ◽  
Vol 51 (1) ◽  
Author(s):  
Shakeel Ahmed Lakho ◽  
Muhammad Haseeb ◽  
Jianmei Huang ◽  
Zhang Yang ◽  
Muhammad Waqqas Hasan ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Immunoblot Analysis ◽  
Eimeria Acervulina ◽  
Ifn Γ ◽  
Negative Controls ◽  
Th1 Polarization ◽  
Dc Maturation

AbstractDendritic cells (DCs) play a pivotal role to amplify antigen-specific immune responses. Antigens that sensitize T cells via antigen-presentation by DCs could enhance the capacity of host immunity to fight infections. In this study, we tested the immunogenic profiles of chicken DCs towards Glyceraldehyde-3-phosphate dehydrogenase from Eimeria acervulina (EaGAPDH). Immunoblot analysis showed that recombinant EaGAPDH (rEaGAPDH) protein was successfully recognized by rat sera generated against rEaGAPDH. Interaction and internalisation of rEaGAPDH by chicken splenic-derived DCs (chSPDCs) was confirmed by immunofluorescence analysis. Flow cytometry revealed that chSPDCs upregulated MHCII, CD1.1, CD11c, CD80, and CD86 cell-surface markers. Moreover, mRNA expressions of DC maturation biomarkers (CCL5, CCR7, and CD83) and TLR signalling genes (TLR15 and MyD88) were also upregulated whereas those of Wnt signalling were non-significant compared to negative controls. rEaGAPDH treatment induced IL-12 and IFN-γ secretion in chSPDCs but had no effect on IL-10 and TGF-β. Likewise, DC-T cell co-culture promoted IFN-γ secretion and the level of IL-4 was unaffected. Proliferation of T cells and their differentiation into CD3+/CD4+ T cells were triggered in chSPDCs-T cells co-culture system. Taken together, rEaGAPDH could promote Th1 polarization by activating both host DCs and T cells and sheds new light on the role of this important molecule which might contribute to the development of new DCs-based immunotherapeutic strategies against coccidiosis.

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