scholarly journals Role of the cytokine environment and cytokine receptor expression on the generation of functionally distinct dendritic cells from human monocytes

2008 ◽  
Vol 38 (3) ◽  
pp. 750-762 ◽  
Author(s):  
Lucia Conti ◽  
Marco Cardone ◽  
Barbara Varano ◽  
Patrizia Puddu ◽  
Filippo Belardelli ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Receptor Expression ◽  
Cytokine Receptor ◽  
Human Monocytes

Role of 4-hydroxynonenal in the hemozoin-mediated inhibition of differentiation of human monocytes to dendritic cells induced by GM-CSF/IL-4

BioFactors ◽  
2005 ◽  
Vol 24 (1-4) ◽  
pp. 283-289 ◽  
Author(s):  
Oleksii Skorokhod ◽  
Evelin Schwarzer ◽  
Tilman Grune ◽  
Paolo Arese
Keyword(s):  
Dendritic Cells ◽  
Human Monocytes ◽  
Gm Csf ◽  
Inhibition Of Differentiation

Oncogenic JAK2V617F requires an intact SH2-like domain for constitutive activation and induction of a myeloproliferative disease in mice

Blood ◽  
2010 ◽  
Vol 116 (22) ◽  
pp. 4600-4611 ◽  
Author(s):  
Sivahari P. Gorantla ◽  
Tobias N. Dechow ◽  
Rebekka Grundler ◽  
Anna Lena Illert ◽  
Christian Meyer zum Büschenfelde ◽  
...  
Keyword(s):  
Myeloproliferative Neoplasms ◽  
Critical Role ◽  
Sh2 Domain ◽  
Receptor Expression ◽  
Cytokine Receptor ◽  
Myeloproliferative Disease ◽  
Constitutive Activation ◽  
Downstream Signaling ◽  
Strict Requirement

Abstract The oncogenic JAK2V617F mutation is found in myeloproliferative neoplasms (MPNs) and is believed to be critical for leukemogenesis. Here we show that JAK2V617F requires an intact SH2 domain for constitutive activation of downstream signaling pathways. In addition, there is a strict requirement of cytokine receptor expression for the activation of this oncogene. Further analysis showed that the SH2 domain mutation did not interfere with JAK2 membrane distribution. However, coimmunoprecipitated experiments revealed a role for the SH2 domain in the aggregation and cross-phosphorylation of JAK2V617F at the cell membrane. Forced overexpression of cytokine receptors could rescue the JAK2V617F SH2 mutant supporting a critical role of JAK2V617F abundance for constitutive activation. However, under physiologic cytokine receptor expression the SH2 domain is absolutely necessary for oncogenic JAK2V617F activation. This is demonstrated in a bone marrow transplantation model, in which an intact SH2 domain in JAK2V617F is required for the induction of an MPN-like disease. Thus, our results points to an indispensable role of the SH2 domain in JAK2V617F-induced MPNs.

Induction of interleukin‐10 expression through Fcα receptor in human monocytes and monocyte‐derived dendritic cells: role of p38 MAPKinase

2010 ◽  
Vol 88 (4) ◽  
pp. 486-493 ◽  
Author(s):  
Charles Pilette ◽  
Bruno Detry ◽  
Amélie Guisset ◽  
Julie Gabriels ◽  
Yves Sibille
Keyword(s):  
Dendritic Cells ◽  
Interleukin 10 ◽  
Human Monocytes

scholarly journals Thymic DCs derived IL-27 regulates the final maturation of CD4+ SP thymocytes

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Hui Tang ◽  
Jie Zhang ◽  
Xiuyuan Sun ◽  
Xiaoping Qian ◽  
Yu Zhang ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Plasmacytoid Dendritic Cells ◽  
Receptor Expression ◽  
Thymocyte Development ◽  
Final Maturation ◽  
Single Positive ◽  
Th17 Differentiation

Abstract IL-27, as a pleiotropic cytokine, promotes the differentiation of naïve T cells to Th1, while suppressing Th2 and Th17 differentiation in the periphery. However, the role of IL-27 in the thymocyte development remains unknown. Here we showed that IL-27 was highly expressed in thymic plasmacytoid dendritic cells (pDCs) while its receptor expression was mainly detected in CD4+ single-positive (SP) thymocytes. Deletion of the p28 subunit in DCs resulted in a reduction of the most mature Qa-2+ subsets of CD4+ SP T cells. This defect was rescued by intrathymic administration of exogenous IL-27. In vitro differentiation assay further demonstrated that IL-27 alone was able to drive the maturation of the newly generated 6C10+CD69+CD4+ SP cells into Qa-2+ cells. Collectively, this study has revealed an important role of thymic DCs-derived IL-27 in the regulation of the phenotypic maturation of CD4+ SP thymocytes.

Role of adenosine receptors in regulating chemotaxis and cytokine production of plasmacytoid dendritic cells

Blood ◽  
2004 ◽  
Vol 103 (4) ◽  
pp. 1391-1397 ◽  
Author(s):  
Max Schnurr ◽  
Tracey Toy ◽  
Amanda Shin ◽  
Gunther Hartmann ◽  
Simon Rothenfusser ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Adenosine Receptors ◽  
Plasmacytoid Dendritic Cells ◽  
Receptor Expression ◽  
Type I ◽  
Cpg Oligodeoxynucleotides ◽  
A2a Receptor ◽  
A1 Receptor ◽  
And Function

Abstract Plasmacytoid dendritic cells (PDCs) are potent regulators of immune function and the major source of type I interferon (IFN) following viral infection. PDCs are found at sites of inflammation in allergic reactions, autoimmune disorders, and cancer, but the mechanisms leading to the recruitment of PDCs to these sites remain elusive. During inflammation, adenosine is released and functions as a signaling molecule via adenosine receptors. This study analyzes adenosine receptor expression and function in human PDCs. Adenosine was found to be a potent chemotactic stimulus for immature PDCs via an A1 receptor–mediated mechanism. The migratory response toward adenosine was comparable to that seen with CXCL12 (stromal-derived factor-1α [SDF-1α), the most potent chemotactic stimulus identified thus far for immature PDCs. Upon maturation, PDCs down-regulate the A1 receptor, resulting in a loss of migratory function. In contrast, mature PDCs up-regulate the A2a receptor, which is positively coupled to adenylyl cyclase and has been implicated in the down-regulation of DC cytokine-producing capacity. We show that in mature PDCs adenosine reduces interleukin-6 (IL-6), IL-12, and IFN-α production in response to CpG oligodeoxynucleotides (ODN). These findings indicate that adenosine may play a dual role in PDC-mediated immunity by initially recruiting immature PDCs to sites of inflammation and by subsequently limiting the extent of the inflammatory response induced by mature PDCs by inhibiting their cytokine-producing capacity.

Interleukin-18 attracts plasmacytoid dendritic cells (DC2s) and promotes Th1 induction by DC2s through IL-18 receptor expression

Blood ◽  
2004 ◽  
Vol 103 (2) ◽  
pp. 648-655 ◽  
Author(s):  
Arthur Kaser ◽  
Susanne Kaser ◽  
Nicole C. Kaneider ◽  
Barbara Enrich ◽  
Christian J. Wiedermann ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Receptor Expression ◽  
Interleukin 18 ◽  
Th2 Response ◽  
Th1 Response ◽  
Initiation Phase ◽  
Helper Cell Type

Abstract In vivo evidence suggests that interleukin-18 (IL-18) shapes the development of adaptive immunity toward T-helper cell type 1 (Th1) responses. Monocyte-derived dendritic cells 1 (DC1s) preferentially induce a Th1 response, while plasmacytoid DC-derived DC2s have been linked to a Th2 response. We analyzed the role of IL-18 during the initiation phase of a Th response in vitro to elucidate the basis of these in vivo observations. IL-18 was constitutively released from DC1s, but not DC2s. Neutralization of IL-18 in coculture experiments of DC1s with allogeneic naive T lymphocytes did not alter the Th1/Th2 phenotype, while anti–IL-12 efficiently down-regulated the Th1 response. Unexpectedly, IL-18 receptor (IL-18R) α and β chains were expressed on DC2 lineage. IL-18R expression was functional, as IL-18 induced chemotaxis in plasmacytoid DCs (pre-DC2s) and enhanced the allostimulatory capacity of IL-3–differentiated DC2s. Pre-DC2s exposed to IL-18 skewed the development of Th cells toward Th1 in coculture experiments of DC2s and allogeneic naive T cells, which was inhibited by IL-12 p70 neutralization. IL-18 might have a profound role during the initiation phase of an immune response by recruiting pre-DC2s and modulating the function of DC2s.

Role of the 807 C/T Polymorphism of the α2 Gene in Platelet GP Ia Collagen Receptor Expression and Function

1999 ◽  
Vol 81 (06) ◽  
pp. 951-956 ◽  
Author(s):  
J. Corral ◽  
R. González-Conejero ◽  
J. Rivera ◽  
F. Ortuño ◽  
P. Aparicio ◽  
...  
Keyword(s):  
Venous Thrombosis ◽  
Cell Types ◽  
Receptor Expression ◽  
Case Control Studies ◽  
Platelet Surface ◽  
Vitro Analysis ◽  
And Function ◽  
In Vitro Analysis

SummaryThe variability of the platelet GP Ia/IIa density has been associated with the 807 C/T polymorphism (Phe 224) of the GP Ia gene in American Caucasian population. We have investigated the genotype and allelic frequencies of this polymorphism in Spanish Caucasians. The T allele was found in 35% of the 284 blood donors analyzed. We confirmed in 159 healthy subjects a significant association between the 807 C/T polymorphism and the platelet GP Ia density. The T allele correlated with high number of GP Ia molecules on platelet surface. In addition, we observed a similar association of this polymorphism with the expression of this protein in other blood cell types. The platelet responsiveness to collagen was determined by “in vitro” analysis of the platelet activation and aggregation response. We found no significant differences in these functional platelet parameters according to the 807 C/T genotype. Finally, results from 3 case/control studies involving 302 consecutive patients (101 with coronary heart disease, 104 with cerebrovascular disease and 97 with deep venous thrombosis) determined that the 807 C/T polymorphism of the GP Ia gene does not represent a risk factor for arterial or venous thrombosis.

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