Performance of cell-penetrating peptide-linked polymers physically mixed with poorly membrane-permeable molecules on cell membranes

2012 ◽  
Vol 81 (1) ◽  
pp. 64-73 ◽  
Author(s):  
Shinji Sakuma ◽  
Masaya Suita ◽  
Takafumi Yamamoto ◽  
Yoshie Masaoka ◽  
Makoto Kataoka ◽  
...  
Keyword(s):  
Cell Membranes ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating

scholarly journals On the mechanisms of the internalization of S413-PV cell-penetrating peptide

2005 ◽  
Vol 390 (2) ◽  
pp. 603-612 ◽  
Author(s):  
Miguel Mano ◽  
Cristina Teodósio ◽  
Artur Paiva ◽  
Sérgio Simões ◽  
Maria C. Pedroso de Lima
Keyword(s):  
Cellular Uptake ◽  
Cell Membranes ◽  
Heparan Sulphate ◽  
Cell Penetrating Peptides ◽  
Membrane Receptors ◽  
Live Cells ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating ◽  
Pv Cell

Cell-penetrating peptides have been shown to translocate across eukaryotic cell membranes through a temperature-insensitive and energy-independent mechanism that does not involve membrane receptors or transporters. Although cell-penetrating peptides have been successfully used to mediate the intracellular delivery of a wide variety of molecules of pharmacological interest both in vitro and in vivo, the mechanisms by which cellular uptake occurs remain unclear. In the face of recent reports demonstrating that uptake of cell-penetrating peptides occurs through previously described endocytic pathways, or is a consequence of fixation artifacts, we conducted a critical re-evaluation of the mechanism responsible for the cellular uptake of the S413-PV karyophilic cell-penetrating peptide. We report that the S413-PV peptide is able to accumulate inside live cells very efficiently through a rapid, dose-dependent and non-toxic process, providing clear evidence that the cellular uptake of this peptide cannot be attributed to fixation artifacts. Comparative analysis of peptide uptake into mutant cells lacking heparan sulphate proteoglycans demonstrates that their presence at the cell surface facilitates the cellular uptake of the S413-PV peptide, particularly at low peptide concentrations. Most importantly, our results clearly demonstrate that, in addition to endocytosis, which is only evident at low peptide concentrations, the efficient cellular uptake of the S413-PV cell-penetrating peptide occurs mainly through an alternative, non-endocytic mechanism, most likely involving direct penetration across cell membranes.

scholarly journals Vesicles mimicking normal and cancer cell membranes exhibit differential responses to the cell-penetrating peptide Pep-1

2018 ◽  
Vol 1860 (6) ◽  
pp. 1394-1402 ◽  
Author(s):  
Bashiyar Almarwani ◽  
Esther Nzuzi Phambu ◽  
Christopher Alexander ◽  
Ha Aimee T. Nguyen ◽  
Nsoki Phambu ◽  
...  
Keyword(s):  
Cancer Cell ◽  
Cell Membranes ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating ◽  
Differential Responses

scholarly journals Designed Cell-Penetrating Peptide Inhibitors of Amyloid-beta Aggregation and Cytotoxicity

2020 ◽  
Vol 1 (2) ◽  
pp. 100014 ◽  
Author(s):  
Anja Henning-Knechtel ◽  
Sunil Kumar ◽  
Cecilia Wallin ◽  
Sylwia Król ◽  
Sebastian K.T.S. Wärmländer ◽  
...  
Keyword(s):  
Amyloid Beta ◽  
Peptide Inhibitors ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating

18F-Labeled phosphopeptide-cell-penetrating peptide dimers with enhanced cell uptake properties in human cancer cells

2012 ◽  
Vol 39 (8) ◽  
pp. 1202-1212 ◽  
Author(s):  
Susan Richter ◽  
Vincent Bouvet ◽  
Melinda Wuest ◽  
Ralf Bergmann ◽  
Joerg Steinbach ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Human Cancer ◽  
Cell Uptake ◽  
Cell Penetrating Peptide ◽  
Human Cancer Cells ◽  
Cell Penetrating
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