scholarly journals FT-IR analysis of Sapium ellipticum (Hochst) pax ethanol leaf extract and its inhibitory effects on pancreatic α-amylase and intestinal α-glucosidase activities in vitro

2016 ◽  
Vol 3 (4) ◽  
pp. 343-349 ◽  
Author(s):  
Osasenaga Macdonald Ighodaro ◽  
Oluseyi Adeboye Akinloye ◽  
Regina Ngozi Ugbaja ◽  
Samuel Olatunbosun Omotainse ◽  
Opeyemi Faokunla
Keyword(s):  
Leaf Extract ◽  
Inhibitory Effects ◽  
Ft Ir ◽  
Ir Analysis

scholarly journals Application of Magnetic Nanoparticles with Structure of Core-shell, as Bactericidal

2018 ◽  
Vol 2 (1) ◽  
pp. 01-04
Author(s):  
Mansour Binandeh
Keyword(s):  
Magnetic Nanoparticles ◽  
Pathogenic Bacteria ◽  
Antibacterial Properties ◽  
Correlation Method ◽  
Core Shell ◽  
Ft Ir ◽  
Chemical Correlation ◽  
Ir Analysis ◽  
The Magnetic Field

Initially, magnetic nanoparticles (MNP) Fe3O4 are synthesized by a chemical correlation method and its core / shell structure is detected using SEM, FT-IR analysis. The purpose of this production was to use the nanoparticle performance level in the absorption of antibiotics, namely, ampicillin (amp). Absorption sampling was analyzed by UV-Vis spectrophotometer and the results indicate that the absorbance of the ampere increases to 85%. The bond between these two is electrostatic bonding, which was confirmed by EDX analysis. Ultimately, this compound was used for the antibacterial process. In this case, the MNP-amp compound was added in a natural amount of 20 μl a bacterial culture pattern overnight (In-vitro). The results showed that 95% of the bacteria were killed (confirmation of antibacterial properties of MNP). Therefore, it can be transmitted intentionally by controlling the magnetic field into living cells for the destruction of pathogenic bacteria.

scholarly journals Phytochemical screening and in-vitro antibacterial and DPPH free radical scavenging activities of methanol extract of root of Combretum album Pers.

2021 ◽  
Vol 8 (4) ◽  
Author(s):  
Sunanda Burman ◽  
Goutam Chandra
Keyword(s):  
Functional Groups ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Chloroform Fraction ◽  
Active Ingredients ◽  
Ic50 Value ◽  
Ft Ir ◽  
Ir Analysis

The objective of the study was to investigate in-vitro antibacterial and antioxidant activities of methanolic extract of Combretum album Pers. root. In brief antibacterial efficacies of methanol extract and its petroleum ether fraction (MePET), chloroform fraction (MeCH), ethyl acetate fraction (MeEA) and water fraction (MeAQ) were determined by agar well diffusion assay along with Minimum Inhibitory Concentrations (MICs). In-vitro antioxidant efficacies were evaluated by DPPH radical scavenging method. Preliminary phytochemical assay, Fourier Transform Infrared Spectroscopy and Gas Chromatography-Mass Spectroscopy analyses were employed to detect the plausible active ingredients. Methanol extract showed broad spectrum antibacterial activity having highest inhibition zone against Staphylococcus aureus MTCC 2940 (21.67±0.58 mm) and MICs ranged from100 µg/ml to 250 µg/ml. The scavenging activity of methanol extract of root of C. album was concentration dependant and IC50 value was 136.08 µg/ml. The lowest MIC (5 µg/ml) was noted with MeCH against B. subtilis (MTCC 441). MeCH showed highest antioxidant activity with an IC50 value of 12.98 ?g/mL and MePET, MeEA, MeAQ and Ascorbic acid presented antioxidant potential with IC50 values of 16.10, 15.07, 17.44 and 13.40??g/mL respectively. Preliminary phytochemical tests and FT-IR analysis revealed presence of various phytochemicals and functional groups like hydroxyl, carboxylic acids, amidines, amines, aromatics and esters. Three compounds were elucidated from bioactive TLC fraction of MeCH in GC-MS analysis supported by presence of various functional groups in MeCH as detected in FT-IR analysis. It is concluded that methanolic extract of C. album root is a potential antibacterial and antioxidant agent where presence of N-[5-[4-pyridinyl)-1H-1,2,4-triazol-3-yl]-benzamide (compound 1), pyruvic acid (compound 2) and methyloacetone (compound 3) are responsible as possible active ingredients.

scholarly journals Application of MNPs-IHSPN nanoparticles in really stabilization of biomolecules bio drugs In-vitro environment

2019 ◽  
Author(s):  
Mansour Binandeh ◽  
Sadegh Rostamnia ◽  
Farrokh Karimi
Keyword(s):  
Magnetic Nanoparticles ◽  
Absorption Rate ◽  
Covalent Bonding ◽  
Living Environment ◽  
Edx Analysis ◽  
Ft Ir ◽  
Ir Analysis ◽  
The Common ◽  
Important Drug

Abstract All scientists are working on biomedicine and Nano-biotechnology. So, one of the most important issues is the study of magnetic nanoparticles (MNPs-IHSPN). The purpose of this project is to examine the approach of using a common buffer to determine the degree of stabilization and release of two molecules of drugs that have been analyzed In-vitro. The structures as a SEM and FT-IR instruments and were used in the amount of 25 mg. After the reaction with biomolecules, the absorption rate was about 60-80%. The release of biomolecules was done by a buffer PBS by spectrophotometer analysis. It is noteworthy that these biomolecules was tested in two forms of covalent and electrostatic bonding. EDX analysis and electrophoresis were used to stabilize the absorption rate in the electrostatic transplant and FT-IR analysis for fixation of covalent bonding. The overall result of this project is to use the common characteristics of some biomolecules for important drug exchange in the living environment of cells.

Phytochemical and antimicrobial analyses of Plectranthus amboinicus leaf extracts

2021 ◽  
pp. 6379-6384
Author(s):  
M.S. Sindhu ◽  
Poonkothai M.
Keyword(s):  
Antimicrobial Activity ◽  
Petroleum Ether ◽  
Functional Groups ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Leaf Extracts ◽  
Fungal Isolates ◽  
Ft Ir ◽  
Ir Analysis ◽  
Plectranthus Amboinicus

The present study is to evaluate the phytochemical constituents and antimicrobial activity of Plectranthus amboinicus leaf extract using petroleum ether, methanol and water as solvents. The antimicrobial activity of P. amboinicus leaf extracts were examined against the selected bacterial and fungal isolates namely Escherichia coli, Staphylococcus aureus and Aspergillus niger, Candida albicans using agar well diffusion method. FT –IR analysis was performed to identify the presence of functional groups in the methanol extract. The results of the study revealed the presence of distinctive active secondary metabolites in the tested leaf extract. The results revealed that the methanol extract exhibited maximum antimicrobial activity against the tested bacterial and fungal isolates when compared with aqueous and petroleum ether extracts. The FT-IR analysis reveals the presence of functional groups such as hydroxyl, amines, alkynes, ketones and carboxylic acid in the methanolic extract of P. amboinicus. Thus, P. amboinicus leaves are effective against the selected microbes and recommends that the plant derived phytochemicals are comparatively safer than synthetic alternative, thereby contributing insightful remedial benefits for the treatment of diseases.

scholarly journals Synthesis of Novel Chitin Derivatives Bearing Amino Groups and Evaluation of Their Antifungal Activity

Marine Drugs ◽  
2018 ◽  
Vol 16 (10) ◽  
pp. 380
Author(s):  
Jingjing Zhang ◽  
Fang Luan ◽  
Qing Li ◽  
Guodong Gu ◽  
Fang Dong ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Inhibitory Effects ◽  
Amino Groups ◽  
Disk Diffusion Method ◽  
Elemental Analyses ◽  
Ft Ir ◽  
Chitin Derivatives

Chemical modification is one of the most effective methods to improve the biological activity of chitin. In the current study, we modified C3-OH and C6-OH of chitin (CT) and successfully synthesized 6-amino-chitin (NCT) and 3,6-diamino-chitin (DNCT) through a series of chemical reactions. The structure of NCT and DNCT were characterized by elemental analyses, FT-IR, 13C NMR, XRD, and SEM. The inhibitory effects of CT, NCT, and DNCT against six kinds of phytopathogen (F. oxysporum f. sp. cucumerium, B. cinerea, C. lagenarium, P. asparagi, F. oxysporum f. niveum, and G. zeae) were evaluated using disk diffusion method in vitro. Meanwhile, carbendazim and amphotericin B were used as positive controls. Results revealed that 6-amino-chitin (NCT) and 3,6-diamino-chitin (DNCT) showed improved antifungal properties compared with pristine chitin. Moreover, DNCT exhibited the better antifungal property than NCT. Especially, while the inhibition zone diameters of NCT are ranged from 11.2 to 16.3 mm, DNCT are about 11.4–20.4 mm. These data demonstrated that the introduction of amino group into chitin derivatives could be key to increasing the antifungal activity of such compounds, and the greater the number of amino groups in the chitin derivatives, the better their antifungal activity was.

scholarly journals PRELIMINARY PHYTOCHEMICAL AND FT-IR ANALYSIS OF METHANOLIC LEAVES EXTRACTS OF HEMIDESMUS INDICUS AND TYLOPHORA INDICA

2021 ◽  
Vol 21 (no 1) ◽  
Author(s):  
M. MAHESHWARI ◽  
P. VIJAYARENGAN
Keyword(s):  
Spectral Analysis ◽  
Bioactive Compounds ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Phytochemical Analysis ◽  
Tylophora Indica ◽  
Traditional Medicines ◽  
Hemidesmus Indicus ◽  
Ft Ir ◽  
Ir Analysis

Medicinal plants are of great importance for the health of individuals and communities. Hemidesmus indicus and Tylophora indica are used as traditional medicines. These plants contain a lot of bioactive compounds. The present investigation is carried out in Hemidesmus indicus and Tylophora indica leaves. In the present study, the methanol extract of H. indicus and T. indica were subjected for preliminary phytochemical analysis through FT-IR spectral analysis and the bioactive compounds were identified. The Preliminary Phytochemical analysis of methanolic leaves extract of H.indicus and T.indica revealed the presence of secondary metabolites. FT-IR analysis of methanolic leaf extract of Hemidesmus indicus and Tylophora indica revealed 16 and 13 major compounds respectively. The H.indicus has higher bioactive compounds compared to T.indica.

scholarly journals Investigation of Chitosan Nanoparticles Loaded with Protocatechuic Acid (PCA) for the Resistance of Pyricularia oryzae Fungus against Rice Blast

Polymers ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 177 ◽  
Author(s):  
The Pham ◽  
Thi Nguyen ◽  
Thuan Thi ◽  
Thanh-Truc Nguyen ◽  
Tien Le ◽  
...  
Keyword(s):  
Rice Blast ◽  
Protocatechuic Acid ◽  
Chitosan Nanoparticles ◽  
Antimicrobial Effect ◽  
Inhibition Zone ◽  
Pyricularia Oryzae ◽  
Transmission Electron ◽  
Ft Ir ◽  
Ir Analysis

In this study, chitosan nanoparticles were used as a carrier for Protocatechuic acid (PCA) to resist Pyricularia oryzae against rice blast. The final compound was characterized using zeta potentials for its surface electricity, Fourier transform infrared (FT-IR) analysis and transmission electron microscopy (TEM) were conducted for functional groups and for particle sizes and shape, respectively. The zeta potential results showed that loading PCA causes chitosan nanoparticle (CSNP) to decrease in surface electrons. The TEM images revealed that the particle size of chitosan (CS), although increasing in size when carrying PCA molecules, showed sufficient size for reasonable penetration into fungal cells. The FT-IR analysis showed that all functional group in CSNP carried PCA matched with previous studies. The antifungal test showed that diameters of inhibition zone of CS increases significantly after loading PCA, exhibiting the strongest antimicrobial effect on the Pyricularia oryzae fungus compared with weaker effects exhibited by CSNP alone or PCA. Our results suggested that CSNP loaded with PCA could be a potential compound for eradication of Pyricularia oryzae and that further testing on in vitro rice plants is recommended to reaffirm this possibility.

scholarly journals Mineralogical and Microstructural Characteristics of Two Dental Pulp Capping Materials

Materials ◽  
2019 ◽  
Vol 12 (11) ◽  
pp. 1772 ◽  
Author(s):  
Georgeta Voicu ◽  
Andreea C. Didilescu ◽  
Andrei B. Stoian ◽  
Cristina Dumitriu ◽  
Maria Greabu ◽  
...  
Keyword(s):  
Resin Matrix ◽  
Microstructural Characteristics ◽  
Average Roughness ◽  
Mineralogical Characterization ◽  
X Ray ◽  
Pulp Capping ◽  
Dental Pulp Capping ◽  
Ft Ir ◽  
Ir Analysis

This paper aims to investigate the composition, surface, and microstructural characteristics, and bioactivity of two commercially available pulp capping materials known as TheraCal LC and BIO MTA+. The materials were prepared as cylindrical samples and assessed by X-ray diffraction (XRD) and complex thermal analysis for mineralogical characterization, and by scanning electron microscopy (SEM) coupled with energy dispersive of X-ray (EDX), Fourier-Transformed Infrared Spectroscopy (FT-IR), and atomic force microscopy (AFM) for microstructural and surface characteristics. The in vitro bioactivity was highlighted by surface mineralization throughout SEM coupled with EDX and FT-IR analysis. XRD analysis performed on both materials showed calcium silicate phases and different radiopacifying compounds. AFM measurements indicated a smoother and more homogenous surface with a lower average roughness for TheraCal LC due to the resin matrix from its composition. FT-IR analysis displayed bands for several compounds in both materials. Both materials exhibited bioactive properties showing surface mineralization after being immersed in solution similar to the human physiological environment. However, the MTA cement showed a better mineralization due to the anhydrous and hydrated phases.

Inhibitory effects of wild bitter melon leaf extract on Propionibacterium acnes-induced skin inflammation in mice and cytokine production in vitro

2015 ◽  
Vol 6 (8) ◽  
pp. 2550-2560 ◽  
Author(s):  
Wen-Cheng Huang ◽  
Tsung-Hsien Tsai ◽  
Ching-Jang Huang ◽  
You-Yi Li ◽  
Jong-Ho Chyuan ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Cytokine Production ◽  
Propionibacterium Acnes ◽  
Skin Inflammation ◽  
Bitter Melon ◽  
Inhibitory Effects

Propionibacterium acnes is a key pathogen involved in acne inflammation.

Sign in / Sign up

Export Citation Format

Share Document