Borrelia burgdorferi sensu lato prevalence and diversity in ticks and small mammals in a Lyme borreliosis endemic Nature Reserve in North-Western Spain. Incidence in surrounding human populations

2017 ◽  
Vol 35 (9) ◽  
pp. 563-568
Author(s):  
Alberto Espí ◽  
Ana Del Cerro ◽  
Aitor Somoano ◽  
Verónica García ◽  
José M. Prieto ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Lyme Borreliosis ◽  
Small Mammals ◽  
Nature Reserve ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Human Populations ◽  
Western Spain ◽  
North Western

scholarly journals A broad-range survey of Borrelia burgdorferi sensu lato infection in small mammals in Yunnan Province, China

2020 ◽  
Author(s):  
Zhihai He ◽  
Baogui Jiang ◽  
Zihou Gao ◽  
Zongti Shao ◽  
Yun Zhang ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Small Mammals ◽  
Yunnan Province ◽  
23S Rrna ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Tropical Region ◽  
Prevalence Rates ◽  
High Genetic Diversity ◽  
Landscape Types ◽  
Pcr Targeting

Abstract Background: Lyme disease, caused by Borrelia burgdorferi sensu lato (BBSL), is commonly found in wild and domestic mammals worldwide. In China, human cases of B. burgdorferi infections have been identified across a wide distribution, but little direct surveillance of potential small mammal reservoirs has been performed in Yunnan Province, a tropical region in southwestern China. Here we report a thorough investigation of BBSL in small mammals collected from 2011 to 2016 from this region.Methods: Small mammals were captured using snap traps in 23 counties located in Yunnan Province. DNA was extracted from spleen tissue using DNA blood and tissue kits. A nested PCR targeting the 5S-23S rRNA intergenic spacer gene of BBSL was used for pathogen detection. Amplicons of 252bp expected sizes were sequenced directly and analyzed using BLAST algorithm. A phylogenetic tree was constructed using MEGA software and statistical analysis were conducted using SPSS version 17.0.Results: Overall, 3659 mammals belonging to 57 species were captured at 159 sample sites located in 23 counties in Yunnan Province. Borrelia burgdorferi s.l. was found in 146 mammals (3.99%), from 30 different species, 20 of which represent the first reported detection in that species. Sequence analysis revealed five genotypes of B. burgdorferi s.l., including B. afzelii, B. burgdorferi sensu stricto, B. japonica, B. garinii and B. valaisiana.Conclusions: Significant differences in prevalence rates of BBSL were observed at varying landscape types and altitudes. Small mammals in forested areas had higher prevalence rates than other landscape types, as did small mammals found at altitudes greater than 2500 meters. The five genotypes of BBSL detected, suggests high genetic diversity within this province.

scholarly journals Differential Transmission of the Genospecies of Borrelia burgdorferi Sensu Lato by Game Birds and Small Rodents in England

1998 ◽  
Vol 64 (4) ◽  
pp. 1169-1174 ◽  
Author(s):  
Klaus Kurtenbach ◽  
Mick Peacey ◽  
Sjoerd G. T. Rijpkema ◽  
Andrew N. Hoodless ◽  
Patricia A. Nuttall ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Lyme Borreliosis ◽  
Sensu Stricto ◽  
Rrna Genes ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Internal Organs ◽  
Borrelia Garinii ◽  
Game Birds ◽  
5S Rrna Genes ◽  
Pcr Targeting

ABSTRACT The genetic diversity of Borrelia burgdorferi sensu lato was assessed in a focus of Lyme borreliosis in southern Britain dominated by game birds. Ticks, rodents, and pheasants were analyzed for spirochete infections by PCR targeting the 23S-5S rRNA genes, followed by genotyping by the reverse line blot method. In questingIxodes ricinus ticks, three genospecies of B. burgdorferi sensu lato were detected, with the highest prevalences found for Borrelia garinii and Borrelia valaisiana. B. burgdorferi sensu stricto was rare (<1%) in all tick stages. Borrelia afzelii was not detected in any of the samples. More than 50% of engorged nymphs collected from pheasants were infected with borreliae, mainly B. garinii and/orB. valaisiana. Although 19% of the rodents harboredB. burgdorferi sensu stricto and/or B. gariniiin internal organs, only B. burgdorferi sensu stricto was transmitted to xenodiagnostic tick larvae (it was transmitted to 1% of the larvae). The data indicate that different genospecies of B. burgdorferi sensu lato can be maintained in nature by distinct transmission cycles involving the same vector tick species but different vertebrate host species. Wildlife management may have an influence on the relative risk of different clinical forms of Lyme borreliosis.

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