scholarly journals Effect of Acoustic Power on In Vivo Molecular Imaging with Targeted Microbubbles: Implications for Low-Mechanical Index Real-Time Imaging

2010 ◽  
Vol 23 (1) ◽  
pp. 79-85 ◽  
Author(s):  
Beat A. Kaufmann ◽  
Chad L. Carr ◽  
Todd Belcik ◽  
Aris Xie ◽  
Benjamin Kron ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Real Time ◽  
Acoustic Power ◽  
Mechanical Index ◽  
Real Time Imaging ◽  
Targeted Microbubbles

scholarly journals Multi-Modal Multi-Spectral Intravital Microscopic Imaging of Signaling Dynamics in Real-Time during Tumor–Immune Interactions

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 499
Author(s):  
Tracy W. Liu ◽  
Seth T. Gammon ◽  
David Piwnica-Worms
Keyword(s):  
Molecular Imaging ◽  
Single Cell ◽  
Real Time ◽  
Bioluminescence Imaging ◽  
Emission Spectra ◽  
Ccd Camera ◽  
Microscopic Imaging ◽  
Signaling Dynamics ◽  
Window Chamber

Intravital microscopic imaging (IVM) allows for the study of interactions between immune cells and tumor cells in a dynamic, physiologically relevant system in vivo. Current IVM strategies primarily use fluorescence imaging; however, with the advances in bioluminescence imaging and the development of new bioluminescent reporters with expanded emission spectra, the applications for bioluminescence are extending to single cell imaging. Herein, we describe a molecular imaging window chamber platform that uniquely combines both bioluminescent and fluorescent genetically encoded reporters, as well as exogenous reporters, providing a powerful multi-plex strategy to study molecular and cellular processes in real-time in intact living systems at single cell resolution all in one system. We demonstrate that our molecular imaging window chamber platform is capable of imaging signaling dynamics in real-time at cellular resolution during tumor progression. Importantly, we expand the utility of IVM by modifying an off-the-shelf commercial system with the addition of bioluminescence imaging achieved by the addition of a CCD camera and demonstrate high quality imaging within the reaches of any biology laboratory.

scholarly journals Quantitative real-time imaging of molecular dynamics during cancer cell invasion and metastasis in vivo

2009 ◽  
Vol 3 (4) ◽  
pp. 351-354 ◽  
Author(s):  
Paul Timpson ◽  
Alan Serrels ◽  
Marta Canel ◽  
Margaret C. Frame ◽  
Valerie G. Brunton ◽  
...  
Keyword(s):  
Molecular Dynamics ◽  
Real Time ◽  
Cancer Cell ◽  
Cell Invasion ◽  
Cancer Cell Invasion ◽  
Invasion And Metastasis ◽  
Real Time Imaging

A near-infrared reversible fluorescent probe for real-time imaging of redox status changes in vivo

2013 ◽  
Vol 4 (3) ◽  
pp. 1079 ◽  
Author(s):  
Kehua Xu ◽  
Mingming Qiang ◽  
Wen Gao ◽  
Ruixian Su ◽  
Na Li ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Redox Status ◽  
Real Time Imaging

Real time imaging reveals a peroxisomal reticulum in living cells

2000 ◽  
Vol 113 (20) ◽  
pp. 3663-3671 ◽  
Author(s):  
M. Schrader ◽  
S.J. King ◽  
T.A. Stroh ◽  
T.A. Schroer
Keyword(s):  
Real Time ◽  
Cytoplasmic Dynein ◽  
Living Cells ◽  
Real Time Imaging ◽  
Peroxisomal Targeting ◽  
Microtubule Motor ◽  
Targeting Signal ◽  
Peroxisomal Targeting Signal

We have directly imaged the dynamic behavior of a variety of morphologically different peroxisomal structures in HepG2 and COS-7 cells transfected with a construct encoding GFP bearing the C-terminal peroxisomal targeting signal 1. Real time imaging revealed that moving peroxisomes interacted with each other and were engaged in transient contacts, and at higher magnification, tubular peroxisomes appeared to form a peroxisomal reticulum. Local remodeling of these structures could be observed involving the formation and detachment of tubular processes that interconnected adjacent organelles. Inhibition of cytoplasmic dynein based motility by overexpression of the dynactin subunit, dynamitin (p50), inhibited the movement of peroxisomes in vivo and interfered with the reestablishment of a uniform distribution of peroxisomes after recovery from nocodazole treatment. Isolated peroxisomes moved in vitro along microtubules in the presence of a microtubule motor fraction. Our data reveal that peroxisomal behavior in vivo is significantly more dynamic and interactive than previously thought and suggest a role for the dynein/dynactin motor in peroxisome motility.

scholarly journals Gold Nanoparticle-Based Fluorescent Theranostics for Real-Time Image-Guided Assessment of DNA Damage and Repair

2019 ◽  
Vol 20 (3) ◽  
pp. 471 ◽  
Author(s):  
Shriya S. Srinivasan ◽  
Rajesh Seenivasan ◽  
Allison Condie ◽  
Stanton L. Gerson ◽  
Yanming Wang ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Real Time ◽  
Targeted Delivery ◽  
Specific Binding ◽  
Imaging Techniques ◽  
Cancer Cell Line ◽  
Molecular Probe ◽  
Biodistribution Studies

Chemotherapeutic dosing, is largely based on the tolerance levels of toxicity today. Molecular imaging strategies can be leveraged to quantify DNA cytotoxicity and thereby serve as a theranostic tool to improve the efficacy of treatments. Methoxyamine-modified cyanine-7 (Cy7MX) is a molecular probe which binds to apurinic/apyrimidinic (AP)-sites, inhibiting DNA-repair mechanisms implicated by cytotoxic chemotherapies. Herein, we loaded (Cy7MX) onto polyethylene glycol-coated gold nanoparticles (AuNP) to selectively and stably deliver the molecular probe intravenously to tumors. We optimized the properties of Cy7MX-loaded AuNPs using optical spectroscopy and tested the delivery mechanism and binding affinity using the DLD1 colon cancer cell line in vitro. A 10:1 ratio of Cy7MX-AuNPs demonstrated a strong AP site-specific binding and the cumulative release profile demonstrated 97% release within 12 min from a polar to a nonpolar environment. We further demonstrated targeted delivery using imaging and biodistribution studies in vivo in an xenografted mouse model. This work lays a foundation for the development of real-time molecular imaging techniques that are poised to yield quantitative measures of the efficacy and temporal profile of cytotoxic chemotherapies.

Fiber-coupled confocal microscope (FCM) for real time imaging of cellular signals in vivo

2006 ◽  
Author(s):  
Takashi Sakurai ◽  
Seiji Yamamoto ◽  
Atsuo Miyakawa ◽  
Yoshihiko Wakazono ◽  
Takato O. Yoshida ◽  
...  
Keyword(s):  
Real Time ◽  
Confocal Microscope ◽  
Real Time Imaging

scholarly journals High-Speed, High-Performance Real-Time Imaging of Physiological Sarcomere Dynamics in the Beating Mouse Heart in Vivo

2016 ◽  
Vol 110 (3) ◽  
pp. 463a
Author(s):  
Fuyu Kobirumaki-Shimozawa ◽  
Kotaro Oyama ◽  
Togo Shimozawa ◽  
Takashi Ohki ◽  
Takako Terui ◽  
...  
Keyword(s):  
Real Time ◽  
High Speed ◽  
High Performance ◽  
Mouse Heart ◽  
Real Time Imaging ◽  
Sarcomere Dynamics

Real-Time Quantitation of Renal Ischemia Using Targeted Microbubbles: In-vivo Measurement of P-selectin Expression

2009 ◽  
Vol 23 (3) ◽  
pp. 373-378 ◽  
Author(s):  
Sero Andonian ◽  
Tonya Coulthard ◽  
Arthur D. Smith ◽  
Pravin S. Singhal ◽  
Benjamin R. Lee
Keyword(s):  
Real Time ◽  
Renal Ischemia ◽  
In Vivo Measurement ◽  
Targeted Microbubbles

scholarly journals S36 Check novel in vivo real time imaging of the bronchial mucosa using an endo-cytoscopy

Thorax ◽  
2010 ◽  
Vol 65 (Suppl 4) ◽  
pp. A18-A19
Author(s):  
K. Shibuya ◽  
N. Okada ◽  
H. Kohno ◽  
N. Iwai ◽  
M. Noro ◽  
...  
Keyword(s):  
Real Time ◽  
Bronchial Mucosa ◽  
Real Time Imaging

scholarly journals In vivo real-time imaging of TGF-?-induced transcriptional activation of the RANK ligand gene promoter in intraosseous prostate cancer

The Prostate ◽  
2004 ◽  
Vol 59 (4) ◽  
pp. 360-369 ◽  
Author(s):  
Jian Zhang ◽  
Yi Lu ◽  
Jinlu Dai ◽  
Zhi Yao ◽  
Riko Kitazawa ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Real Time ◽  
Transcriptional Activation ◽  
Gene Promoter ◽  
Rank Ligand ◽  
Real Time Imaging
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