scholarly journals Interaction of prostate carcinoma-associated fibroblasts with human epithelial cell lines in vivo

2017 ◽  
Vol 96 ◽  
pp. 40-48 ◽  
Author(s):  
Takeshi Sasaki ◽  
Omar E. Franco ◽  
Simon W. Hayward
Keyword(s):  
Epithelial Cell ◽  
Cell Lines ◽  
Prostate Carcinoma ◽  
Human Epithelial Cell ◽  
Epithelial Cell Lines ◽  
Carcinoma Associated Fibroblasts

Bcl-2 protein localizes to the chromosomes of mitotic nuclei and is correlated with the cell cycle in cultured epithelial cell lines

1994 ◽  
Vol 107 (2) ◽  
pp. 363-371
Author(s):  
Q.L. Lu ◽  
A.M. Hanby ◽  
M.A. Nasser Hajibagheri ◽  
S.E. Gschmeissner ◽  
P.J. Lu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Epithelial Cell ◽  
Cell Lines ◽  
Cytoplasmic Localization ◽  
Human Epithelial Cell ◽  
Daughter Cells ◽  
Epithelial Cell Lines ◽  
Cell Immortalization

bcl-2 gene expression confers a survival advantage by preventing cells from entering apoptosis. In contrast to the previously described cytoplasmic localization of Bcl-2 in epithelial cells in vivo, in this study we have demonstrated, in a series of human epithelial cell lines, that Bcl-2 also localizes to mitotic nuclei. Both immunocytochemical and immunoelectron microscopical examinations localize this protein to nuclei and in particular to chromosomes. Nuclear Bcl-2 expression in these cell lines is correlated with the cell cycle. There is relatively strong expression during mitosis, most intense during prophase and metaphase, declining in telophase and then the protein becomes undetectable soon after separation of the two daughter cells. The expression and distribution of Bcl-2 is influenced by treatment with excessive thymidine. These results indicate that Bcl-2 may protect the cells from apoptosis occurring during mitosis and suggest a possible role for the protein in cell immortalization.

Infection of Human Epithelial Cell Lines by Epstein-Barr Virus

ORL ◽  
1984 ◽  
Vol 46 (4) ◽  
pp. 195-201 ◽  
Author(s):  
Tom Takimoto ◽  
Kazuo Morishita ◽  
Ryozo Umeda ◽  
Hisashi Ogura ◽  
Motoichi Hatano
Keyword(s):  
Epithelial Cell ◽  
Cell Lines ◽  
Epstein Barr Virus ◽  
Human Epithelial Cell ◽  
Barr Virus ◽  
Epithelial Cell Lines ◽  
Epstein Barr

Cytokine-mediated PGE2expression in human colonic fibroblasts

1998 ◽  
Vol 275 (4) ◽  
pp. C988-C994 ◽  
Author(s):  
Edward C. Kim ◽  
Yingting Zhu ◽  
Valerie Andersen ◽  
Daniela Sciaky ◽  
H. James Cao ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Lines ◽  
Shape Change ◽  
Cell Types ◽  
Mrna Levels ◽  
Primary Fibroblast ◽  
Fibroblast Cultures ◽  
Epithelial Cell Lines ◽  
Factor Α

We investigated prostanoid biogenesis in human colonic fibroblasts (CCD-18Co and 5 primary fibroblast cultures) and epithelial cell lines (NCM460, T84, HT-29, and LS 174T) and the effect of PGE2 on fibroblast morphology. Cytokine-stimulated PGE2production was measured. PGH synthase-1 and -2 (PGHS-1 and -2) protein and mRNA expression were evaluated. Basal PGE2 levels were low in all cell types (0.15–6.47 ng/mg protein). Treatment for 24 h with interleukin-1β (IL-1β; 10 ng/ml) or tumor necrosis factor-α (50 ng/ml), respectively, elicited maximal 25- and 6-fold inductions of PGE2 synthesis in CCD-18Co cultures and similar results in primary fibroblast cultures; maximal inductions with IL-1β in colonic epithelial cell lines were from zero to fivefold. Treatment of CCD-18Co fibroblasts with IL-1β caused maximal 21- and 53-fold increases, respectively, in PGHS-2 protein and mRNA levels without altering PGHS-1 expression. PGE2 (0.1 μmol/l) elicited a dramatic shape change in selected fibroblasts. Colonic fibroblasts are potentially important as cytokine targets and a source of and target for colonic prostanoids in vivo.

scholarly journals Genes of non-typeable Haemophilus influenzae expressed during interaction with human epithelial cell lines

2002 ◽  
Vol 45 (2) ◽  
pp. 485-500 ◽  
Author(s):  
Peter van Ulsen ◽  
Muriel van schilfgaarde ◽  
Jacob Dankert ◽  
Henk Jansen ◽  
Loek van Alphen
Keyword(s):  
Epithelial Cell ◽  
Haemophilus Influenzae ◽  
Cell Lines ◽  
Human Epithelial Cell ◽  
Epithelial Cell Lines

scholarly journals Cell- and inducer-specific accretion of human isometallothioneins

1993 ◽  
Vol 292 (2) ◽  
pp. 551-554 ◽  
Author(s):  
M Cavigelli ◽  
J H R Kägi ◽  
P E Hunziker
Keyword(s):  
Epithelial Cell ◽  
Cell Lines ◽  
Dose Response ◽  
Zinc Ions ◽  
Human Epithelial Cell ◽  
Germ Layers ◽  
Neutral Ph ◽  
Epithelial Cell Lines

The synthesis of metallothionein (MT) was investigated in three different human epithelial cell lines, each derived from one of the embryonic germ layers. The accretion of different isoforms of the protein was monitored using a sensitive neutral-pH h.p.l.c. method. Induction of MT synthesis by zinc ions and dexamethasone revealed differences between the three cell lines, both with respect to the number and the amounts of the different isoMTs formed. Dose-response experiments showed that an increase in dexamethasone concentration enhances MT accretion asymptotically to a limit, whereas within the concentration range explored zinc produces an exponential augmentation.

scholarly journals Regulation ofBRCA1messenger RNA stability in human epithelial cell lines and during cell cycle progression

FEBS Letters ◽  
2007 ◽  
Vol 581 (18) ◽  
pp. 3435-3442 ◽  
Author(s):  
Jodi M. Saunus ◽  
Stacey L. Edwards ◽  
Juliet D. French ◽  
Chanel E. Smart ◽  
Melissa A. Brown
Keyword(s):  
Cell Cycle ◽  
Epithelial Cell ◽  
Cell Lines ◽  
Cell Cycle Progression ◽  
Rna Stability ◽  
Human Epithelial Cell ◽  
Cycle Progression ◽  
Epithelial Cell Lines
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