Angiotensin II promotes cardiac differentiation of embryonic stem cells via angiotensin type 1 receptor

2013 ◽  
Vol 86 (1-2) ◽  
pp. 23-29 ◽  
Author(s):  
Liyuan Wu ◽  
Zhuqing Jia ◽  
Lihui Yan ◽  
Weiping Wang ◽  
Jiaji Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Angiotensin Ii ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cardiac Differentiation ◽  
Angiotensin Type

Angiotensin II type 1 and bradykinin B2 receptors expressed in early stage epithelial cells derived from human embryonic stem cells

2007 ◽  
Vol 211 (3) ◽  
pp. 816-825 ◽  
Author(s):  
Zhenhua Huang ◽  
Jun Yu ◽  
Paul Toselli ◽  
Jag Bhawan ◽  
Vasanthi Sudireddy ◽  
...  
Keyword(s):  
Stem Cells ◽  
Angiotensin Ii ◽  
Embryonic Stem Cells ◽  
Epithelial Cells ◽  
Human Embryonic Stem Cells ◽  
Early Stage ◽  
Embryonic Stem ◽  
B2 Receptors

scholarly journals 5-azacytidine induces cardiac differentiation of P19 embryonic stem cells

2004 ◽  
Vol 36 (6) ◽  
pp. 515-523 ◽  
Author(s):  
Seung-Cheol Choi ◽  
Jihyun Yoon ◽  
Wan-Joo Shim ◽  
Young-Moo Ro ◽  
Do-Sun Lim
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cardiac Differentiation

scholarly journals Ouabain facilitates cardiac differentiation of mouse embryonic stem cells through ERK1/2 pathway

2010 ◽  
Vol 32 (1) ◽  
pp. 52-61 ◽  
Author(s):  
Yee-Ki Lee ◽  
Kwong-Man Ng ◽  
Wing-Hon Lai ◽  
Cornelia Man ◽  
Deborah K Lieu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Cardiac Differentiation

scholarly journals Rapamycin efficiently promotes cardiac differentiation of mouse embryonic stem cells

2017 ◽  
Vol 37 (3) ◽  
Author(s):  
Qin Lu ◽  
Yinan Liu ◽  
Yang Wang ◽  
Weiping Wang ◽  
Zhe Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Early Stage ◽  
Mammalian Target Of Rapamycin ◽  
Embryonic Stem ◽  
Cardiac Differentiation ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein ◽  
Cardiac Transcription Factors ◽  
20 Nm

To investigate the effects of rapamycin on cardiac differentiation, murine embryonic stem cells (ESCs) were induced into cardiomyocytes by 10−4 M ascorbic acid (AA), 20 nM rapamycin alone or 0.01% solvent DMSO. We found that rapamycin alone was insufficient to initiate cardiomyogenesis. Then, the ESCs were treated with AA and rapamycin (20 nM) or AA and DMSO (0.01%) as a control. Compared with control, mouse ESCs (mESCs) treated with rapamycin (20 nM) and AA yielded a significantly higher percentage of cardiomyocytes, as confirmed by the percentage of beating embryonic bodies (EBs), the immunofluorescence and FACS analysis. Rapamycin significantly increased the expression of a panel of cardiac markers including Gata4, α-Mhc, β-Mhc, and Tnnt2. Additionally, rapamycin enhanced the expression of mesodermal and cardiac transcription factors such as Mesp1, Brachyury T, Eomes, Isl1, Gata4, Nkx2.5, Tbx5, and Mef2c. Mechanistic studies showed that rapamycin inhibits Wnt/β-catenin and Notch signaling but promotes the expression of fibroblast growth factor (Fgf8), Fgf10, and Nodal at early stage, and bone morphogenetic protein 2 (Bmp 2) at later stages. Sequential treatment of rapamycin showed that rapamycin promotes cardiac differentiation at the early and later stages. Interestingly, another mammalian target of rapamycin (mTOR) inhibitor Ku0063794 (1 µM) had similar effects on cardiomyogenesis. In conclusion, our results highlight a practical approach to generate cardiomyocytes from mESCs by rapamycin.

scholarly journals Minimal contribution of IP3R2 in cardiac differentiation and derived ventricular-like myocytes from human embryonic stem cells

2020 ◽  
Vol 41 (12) ◽  
pp. 1576-1586
Author(s):  
Peng Zhang ◽  
Ji-jun Huang ◽  
Kun-fu Ou-yang ◽  
He Liang ◽  
Miao-ling Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cardiac Differentiation

Enrichment of cardiac differentiation of mouse embryonic stem cells by optimizing the hanging drop method

2010 ◽  
Vol 33 (4) ◽  
pp. 853-858 ◽  
Author(s):  
Ming Chen ◽  
Yong-Qing Lin ◽  
Shuang-Lun Xie ◽  
Hong-Fu Wu ◽  
Jing-Feng Wang
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Cardiac Differentiation ◽  
Hanging Drop ◽  
Drop Method ◽  
Hanging Drop Method

scholarly journals Implications of Glucose Transporter Protein Type 1 (GLUT1)-Haplodeficiency in Embryonic Stem Cells for Their Survival in Response to Hypoxic Stress

2003 ◽  
Vol 163 (5) ◽  
pp. 1873-1885 ◽  
Author(s):  
Charles Heilig ◽  
Frank Brosius ◽  
Brian Siu ◽  
Luis Concepcion ◽  
Richard Mortensen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Glucose Transporter ◽  
Embryonic Stem ◽  
Hypoxic Stress ◽  
Transporter Protein
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