scholarly journals 5-azacytidine induces cardiac differentiation of P19 embryonic stem cells

2004 ◽  
Vol 36 (6) ◽  
pp. 515-523 ◽  
Author(s):  
Seung-Cheol Choi ◽  
Jihyun Yoon ◽  
Wan-Joo Shim ◽  
Young-Moo Ro ◽  
Do-Sun Lim
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cardiac Differentiation

scholarly journals Ouabain facilitates cardiac differentiation of mouse embryonic stem cells through ERK1/2 pathway

2010 ◽  
Vol 32 (1) ◽  
pp. 52-61 ◽  
Author(s):  
Yee-Ki Lee ◽  
Kwong-Man Ng ◽  
Wing-Hon Lai ◽  
Cornelia Man ◽  
Deborah K Lieu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Cardiac Differentiation

scholarly journals Rapamycin efficiently promotes cardiac differentiation of mouse embryonic stem cells

2017 ◽  
Vol 37 (3) ◽  
Author(s):  
Qin Lu ◽  
Yinan Liu ◽  
Yang Wang ◽  
Weiping Wang ◽  
Zhe Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Early Stage ◽  
Mammalian Target Of Rapamycin ◽  
Embryonic Stem ◽  
Cardiac Differentiation ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein ◽  
Cardiac Transcription Factors ◽  
20 Nm

To investigate the effects of rapamycin on cardiac differentiation, murine embryonic stem cells (ESCs) were induced into cardiomyocytes by 10−4 M ascorbic acid (AA), 20 nM rapamycin alone or 0.01% solvent DMSO. We found that rapamycin alone was insufficient to initiate cardiomyogenesis. Then, the ESCs were treated with AA and rapamycin (20 nM) or AA and DMSO (0.01%) as a control. Compared with control, mouse ESCs (mESCs) treated with rapamycin (20 nM) and AA yielded a significantly higher percentage of cardiomyocytes, as confirmed by the percentage of beating embryonic bodies (EBs), the immunofluorescence and FACS analysis. Rapamycin significantly increased the expression of a panel of cardiac markers including Gata4, α-Mhc, β-Mhc, and Tnnt2. Additionally, rapamycin enhanced the expression of mesodermal and cardiac transcription factors such as Mesp1, Brachyury T, Eomes, Isl1, Gata4, Nkx2.5, Tbx5, and Mef2c. Mechanistic studies showed that rapamycin inhibits Wnt/β-catenin and Notch signaling but promotes the expression of fibroblast growth factor (Fgf8), Fgf10, and Nodal at early stage, and bone morphogenetic protein 2 (Bmp 2) at later stages. Sequential treatment of rapamycin showed that rapamycin promotes cardiac differentiation at the early and later stages. Interestingly, another mammalian target of rapamycin (mTOR) inhibitor Ku0063794 (1 µM) had similar effects on cardiomyogenesis. In conclusion, our results highlight a practical approach to generate cardiomyocytes from mESCs by rapamycin.

scholarly journals Minimal contribution of IP3R2 in cardiac differentiation and derived ventricular-like myocytes from human embryonic stem cells

2020 ◽  
Vol 41 (12) ◽  
pp. 1576-1586
Author(s):  
Peng Zhang ◽  
Ji-jun Huang ◽  
Kun-fu Ou-yang ◽  
He Liang ◽  
Miao-ling Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cardiac Differentiation

Enrichment of cardiac differentiation of mouse embryonic stem cells by optimizing the hanging drop method

2010 ◽  
Vol 33 (4) ◽  
pp. 853-858 ◽  
Author(s):  
Ming Chen ◽  
Yong-Qing Lin ◽  
Shuang-Lun Xie ◽  
Hong-Fu Wu ◽  
Jing-Feng Wang
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Cardiac Differentiation ◽  
Hanging Drop ◽  
Drop Method ◽  
Hanging Drop Method

scholarly journals The microwell control of embryoid body size in order to regulate cardiac differentiation of human embryonic stem cells

Biomaterials ◽  
2010 ◽  
Vol 31 (7) ◽  
pp. 1885-1893 ◽  
Author(s):  
Jeffrey C. Mohr ◽  
Jianhua Zhang ◽  
Samira M. Azarin ◽  
Andrew G. Soerens ◽  
Juan J. de Pablo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Body Size ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryoid Body ◽  
Embryonic Stem ◽  
Cardiac Differentiation

Effects of iron oxide nanoparticles on cardiac differentiation of embryonic stem cells

2009 ◽  
Vol 379 (4) ◽  
pp. 898-903 ◽  
Author(s):  
Ka-Wing Au ◽  
Song-Yan Liao ◽  
Yee-Ki Lee ◽  
Wing-Hon Lai ◽  
Kwong-Man Ng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Iron Oxide ◽  
Embryonic Stem Cells ◽  
Iron Oxide Nanoparticles ◽  
Embryonic Stem ◽  
Cardiac Differentiation ◽  
Oxide Nanoparticles
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