Extracellular matrix protein mediated regulation of the osteoblast differentiation of bone marrow derived human mesenchymal stem cells

2012 ◽  
Vol 84 (2) ◽  
pp. 185-192 ◽  
Author(s):  
Smitha Mathews ◽  
Ramesh Bhonde ◽  
Pawan Kumar Gupta ◽  
Satish Totey
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Extracellular Matrix ◽  
Mesenchymal Stem Cells ◽  
Osteoblast Differentiation ◽  
Matrix Protein ◽  
Human Mesenchymal Stem Cells ◽  
Extracellular Matrix Protein

Annexin II Mediates Plasminogen-Dependant Matrix Invasion by Adult Human Mesenchymal Stem Cells.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 2326-2326
Author(s):  
Paul B. Bolno ◽  
Doris A. Morgan ◽  
Mahesh Sharma ◽  
Martin Lazorik ◽  
Andrew S. Wechsler ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Extracellular Matrix ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Western Blot ◽  
Human Mesenchymal Stem Cells ◽  
Angiogenic Factors ◽  
Annexin Ii ◽  
Adult Human

Abstract Background: Annexin II (ANX2) is a fibrinolytic receptor that serves as a binding site for plasminogen and tissue plasminogen activator, facilitating the generation of plasmin. ANX2 is present on a wide variety of cells including vascular endothelial cells as well as macrophages. ANX2 has been shown to play a key role in extracellular matrix degradation, cellular migration, and invasion. This degradation of extracellular matrix may also cause the release of matrix-bound angiogenic factors such as VEGF and FGF. We hypothesized that adult human mesenchymal stem cells (hMSCs) express ANX2 and utilize this receptor for plasmin generation to facilitate basement membrane invasion. Methods: Primary hMSCs were isolated from the sternal bone marrow of patients undergoing median sternotomy. Stem cell surface markers were characterized via immuno-fluorescence. The presence of ANX2 protein by hMSCs was established via western blot. ANX2 mediated plasminogen activation and plasmin generation was quantified using chromozyme-P as a colorimetric substrate. Invasion assays were performed in dual-chamber culture wells containing matrigel inserts. hMSCs were plated into upper chambers containing: serum-free medium (SFM), SFM + Plasminogen, or SFM + Plasminogen + epsilon-aminocaproic acid (e-ACA inhibits binding of plasminogen to ANX2). After 24 hours, invasive cells were isolated and counted. Results: Sternal bone marrow derived hMSCs expressed the membrane phenotype CD34 (−), CD14 (−), CD44 (+), CD105 (+), CD106 (+). The presence of ANX2 was confirmed by western blot analysis. hMSCs generated 1.95 units of plasmin per milligram of protein. There was a 20% (p 0 .004) increase in hMSC invasion in the wells containing plasminogen as compared to SFM alone. When e-ACA was introduced there was a decrease in hMSC invasion back to control values. Conclusion: Our observations establish for the first time the presence and functional activity of ANX2 in hMSCs. These data suggest that mesenchymal stem cell expression of ANX2 facilitates plasminogen-mediated hMSC trans-endothelial invasion, migration and the release of pro-angiogenic factors from within the extracellular matrix, promoting stem cell directed repair and angiogenesis.

Differentiation of Rat Mesenchymal Stem Cells toward Osteogenic Lineage on Extracellular Matrix Protein Gradients

2019 ◽  
Vol 8 (17) ◽  
pp. 1900595 ◽  
Author(s):  
Soraya Rasi Ghaemi ◽  
Bahman Delalat ◽  
Alex Cavallaro ◽  
Agnieszka Mierczynska‐Vasilev ◽  
Krasimir Vasilev ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Mesenchymal Stem Cells ◽  
Matrix Protein ◽  
Extracellular Matrix Protein ◽  
Osteogenic Lineage ◽  
Protein Gradients

scholarly journals Gene expression profiling of human mesenchymal stem cells derived from bone marrow during expansion and osteoblast differentiation

BMC Genomics ◽  
2007 ◽  
Vol 8 (1) ◽  
pp. 70 ◽  
Author(s):  
Birgit Kulterer ◽  
Gerald Friedl ◽  
Anita Jandrositz ◽  
Fatima Sanchez-Cabo ◽  
Andreas Prokesch ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Osteoblast Differentiation ◽  
Human Mesenchymal Stem Cells

Glycosaminoglycans enhance osteoblast differentiation of bone marrow derived human mesenchymal stem cells

2012 ◽  
Vol 8 (2) ◽  
pp. 143-152 ◽  
Author(s):  
Smitha Mathews ◽  
Suja Ann Mathew ◽  
Pawan Kumar Gupta ◽  
Ramesh Bhonde ◽  
Satish Totey
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Osteoblast Differentiation ◽  
Human Mesenchymal Stem Cells
Sign in / Sign up

Export Citation Format

Share Document