scholarly journals Extracellular matrix protein adsorption to phosphate-functionalized gels from serum promotes osteogenic differentiation of human mesenchymal stem cells

2013 ◽  
Vol 9 (1) ◽  
pp. 4525-4534 ◽  
Author(s):  
Navakanth R. Gandavarapu ◽  
Peter D. Mariner ◽  
Michael P. Schwartz ◽  
Kristi S. Anseth
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Protein Adsorption ◽  
Matrix Protein ◽  
Human Mesenchymal Stem Cells ◽  
Extracellular Matrix Protein

Differentiation of Rat Mesenchymal Stem Cells toward Osteogenic Lineage on Extracellular Matrix Protein Gradients

2019 ◽  
Vol 8 (17) ◽  
pp. 1900595 ◽  
Author(s):  
Soraya Rasi Ghaemi ◽  
Bahman Delalat ◽  
Alex Cavallaro ◽  
Agnieszka Mierczynska‐Vasilev ◽  
Krasimir Vasilev ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Mesenchymal Stem Cells ◽  
Matrix Protein ◽  
Extracellular Matrix Protein ◽  
Osteogenic Lineage ◽  
Protein Gradients

scholarly journals The Effects of Different Dynamic Culture Systems on Cell Proliferation and Osteogenic Differentiation in Human Mesenchymal Stem Cells

2019 ◽  
Vol 20 (16) ◽  
pp. 4024
Author(s):  
Hsiou-Hsin Tsai ◽  
Kai-Chiang Yang ◽  
Meng-Huang Wu ◽  
Jung-Chih Chen ◽  
Ching-Li Tseng
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Cell Proliferation ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Flow Reactor ◽  
Human Mesenchymal Stem Cells ◽  
Spinner Flask ◽  
Dynamic Culture ◽  
Bidirectional Flow

The culture environment plays an important role for stem cells’ cultivation. Static or dynamic culture preserve differential potentials to affect human mesenchymal stem cells’ (hMSCs) proliferation and differentiation. In this study, hMSCs were seeded on fiber disks and cultured in a bidirectional-flow bioreactor or spinner-flask bioreactor with a supplement of osteogenic medium. The hMSCs’ proliferation, osteogenic differentiation, and extracellular matrix deposition of mineralization were demonstrated. The results showed that the spinner flask improved cell viability at the first two weeks while the bidirectional-flow reactor increased the cell proliferation of hMSCs through the four-week culture period. Despite the flow reactor having a higher cell number, a lower lactose/glucose ratio was noted, revealing that the bidirectional-flow bioreactor provides better oxygen accessibility to the cultured cells/disk construct. The changes of calcium ions in the medium, the depositions of Ca2+ in the cells/disk constructs, and alkaline phosphate/osteocalcin activities showed the static culture of hMSCs caused cells to mineralize faster than the other two bioreactors but without cell proliferation. Otherwise, cells were distributed uniformly with abundant extracellular matrix productions using the flow reactor. This reveals that the static and dynamic cultivations regulated the osteogenic process differently in hMSCs. The bidirectional-flow bioreactor can be used in the mass production and cultivation of hMSCs for applications in bone regenerative medicine.

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