Notch signalling is required for the survival of epithelial stem cells in the continuously growing mouse incisor

2010 ◽  
Vol 80 (4-5) ◽  
pp. 241-248 ◽  
Author(s):  
Szabolcs Felszeghy ◽  
Marika Suomalainen ◽  
Irma Thesleff
Keyword(s):  
Stem Cells ◽  
Notch Signalling ◽  
Epithelial Stem Cells ◽  
Mouse Incisor

scholarly journals Characterization of Dental Epithelial Stem Cells from the Mouse Incisor with Two-Dimensional and Three-Dimensional Platforms

2013 ◽  
Vol 19 (1) ◽  
pp. 15-24 ◽  
Author(s):  
Miquella G. Chavez ◽  
Wenli Yu ◽  
Brian Biehs ◽  
Hidemitsu Harada ◽  
Malcolm L. Snead ◽  
...  
Keyword(s):  
Stem Cells ◽  
Three Dimensional ◽  
Two Dimensional ◽  
Epithelial Stem Cells ◽  
Mouse Incisor

scholarly journals Localization of Putative Stem Cells in Dental Epithelium and Their Association with Notch and Fgf Signaling

1999 ◽  
Vol 147 (1) ◽  
pp. 105-120 ◽  
Author(s):  
Hidemitsu Harada ◽  
Päivi Kettunen ◽  
Han-Sung Jung ◽  
Tuija Mustonen ◽  
Y. Alan Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Epithelial Cells ◽  
Cell Lineage ◽  
Fgf Signaling ◽  
Epithelial Stem Cells ◽  
Lunatic Fringe ◽  
Reticulum Cells ◽  
Stellate Reticulum ◽  
Mouse Incisor ◽  
Basal Epithelial Cells

The continuously growing mouse incisor is an excellent model to analyze the mechanisms for stem cell lineage. We designed an organ culture method for the apical end of the incisor and analyzed the epithelial cell lineage by 5-bromo-2′-deoxyuridine and DiI labeling. Our results indicate that stem cells reside in the cervical loop epithelium consisting of a central core of stellate reticulum cells surrounded by a layer of basal epithelial cells, and that they give rise to transit-amplifying progeny differentiating into enamel forming ameloblasts. We identified slowly dividing cells among the Notch1-expressing stellate reticulum cells in specific locations near the basal epithelial cells expressing lunatic fringe, a secretory molecule modulating Notch signaling. It is known from tissue recombination studies that in the mouse incisor the mesenchyme regulates the continuous growth of epithelium. Expression of Fgf-3 and Fgf-10 were restricted to the mesenchyme underlying the basal epithelial cells and the transit-amplifying cells expressing their receptors Fgfr1b and Fgfr2b. When FGF-10 protein was applied with beads on the cultured cervical loop epithelium it stimulated cell proliferation as well as expression of lunatic fringe. We present a model in which FGF signaling from the mesenchyme regulates the Notch pathway in dental epithelial stem cells via stimulation of lunatic fringe expression and, thereby, has a central role in coupling the mitogenesis and fate decision of stem cells.

scholarly journals Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor

10.3791/51266 ◽  
2014 ◽  
Author(s):  
Miquella G. Chavez ◽  
Jimmy Hu ◽  
Kerstin Seidel ◽  
Chunying Li ◽  
Andrew Jheon ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adult Mouse ◽  
Epithelial Stem Cells ◽  
Mouse Incisor

scholarly journals In vivo administration of dental epithelial stem cells at the apical end of the mouse incisor

2015 ◽  
Vol 6 ◽  
Author(s):  
Giovanna Orsini ◽  
Lucia Jimenez-Rojo ◽  
Despoina Natsiou ◽  
Angelo Putignano ◽  
Thimios A. Mitsiadis
Keyword(s):  
Stem Cells ◽  
Epithelial Stem Cells ◽  
Mouse Incisor ◽  
In Vivo Administration

scholarly journals E-cadherin regulates the behavior and fate of epithelial stem cells and their progeny in the mouse incisor

2012 ◽  
Vol 366 (2) ◽  
pp. 357-366 ◽  
Author(s):  
Chun-Ying Li ◽  
Wanghee Cha ◽  
Hans-Ulrich Luder ◽  
Roch-Philippe Charles ◽  
Martin McMahon ◽  
...  
Keyword(s):  
Stem Cells ◽  
Epithelial Stem Cells ◽  
Mouse Incisor ◽  
E Cadherin

A common mechanism links activities of butyrate in the colon

2017 ◽  
Author(s):  
Mohit S. Verma ◽  
Michael J. Fink ◽  
Gabriel L Salmon ◽  
Nadine Fornelos ◽  
Takahiro E. Ohara ◽  
...  
Keyword(s):  
Stem Cells ◽  
Histone Deacetylases ◽  
Biological Activities ◽  
Short Chain Fatty Acids ◽  
Common Mechanism ◽  
Epithelial Stem Cells ◽  
Degree Of Unsaturation ◽  
Structure Activity ◽  
Starting Point ◽  
New Compounds

Two biological activities of butyrate in the colon (suppression of proliferation of colonic epithelial stem cells and inflammation) correlate with inhibition of histone deacetylases. Cellular and biochemical studies of molecules similar in structure to butyrate, but different in molecular details (functional groups, chain-length, deuteration, oxidation level, fluorination, or degree of unsaturation) demonstrated that these activities were sensitive to molecular structure, and were compatible with the hypothesis that butyrate acts by binding to the Zn<sup>2+</sup> in the catalytic site of histone deacetylases. Structure-activity relationships drawn from a set of 36 compounds offer a starting point for the design of new compounds targeting the inhibition of histone deacetylases. The observation that butyrate was more potent than other short-chain fatty acids is compatible with the hypothesis that crypts evolved (at least in part), to separate stem cells at the base of crypts from butyrate produced by commensal bacteria.

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