scholarly journals Drosophila Ovarian Germline Stem Cell Cytocensor Projections Dynamically Receive and Attenuate BMP Signaling

2019 ◽  
Vol 50 (3) ◽  
pp. 296-312.e5 ◽  
Author(s):  
Scott G. Wilcockson ◽  
Hilary L. Ashe
Keyword(s):  
Stem Cell ◽  
Bmp Signaling ◽  
Germline Stem Cell

scholarly journals The Wnt pathway limits BMP signaling outside of the germline stem cell niche in Drosophila ovaries

2016 ◽  
Vol 417 (1) ◽  
pp. 50-62 ◽  
Author(s):  
Violaine I. Mottier-Pavie ◽  
Victor Palacios ◽  
Susan Eliazer ◽  
Shane Scoggin ◽  
Michael Buszczak
Keyword(s):  
Stem Cell ◽  
Stem Cell Niche ◽  
Wnt Pathway ◽  
Bmp Signaling ◽  
Germline Stem Cell ◽  
Cell Niche ◽  
Germline Stem Cell Niche

scholarly journals The Osa-Containing SWI/SNF Chromatin-Remodeling Complex Is Required in the Germline Differentiation Niche for Germline Stem Cell Progeny Differentiation

Genes ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 363
Author(s):  
Xiaolong Hu ◽  
Mengjie Li ◽  
Xue Hao ◽  
Yi Lu ◽  
Lei Zhang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Bmp Signaling ◽  
Germline Stem Cell ◽  
Dependent Manner ◽  
Self Renewal ◽  
Cellular Processes ◽  
Lineage Differentiation ◽  
Chromatin Remodeling Complex ◽  
Germline Differentiation ◽  
Drosophila Ovary

The Drosophila ovary is recognized as a powerful model to study stem cell self-renewal and differentiation. Decapentaplegic (Dpp) is secreted from the germline stem cell (GSC) niche to activate Bone Morphogenic Protein (BMP) signaling in GSCs for their self-renewal and is restricted in the differentiation niche for daughter cell differentiation. Here, we report that Switch/sucrose non-fermentable (SWI/SNF) component Osa depletion in escort cells (ECs) results in a blockage of GSC progeny differentiation. Further molecular and genetic analyses suggest that the defective germline differentiation is partially attributed to the elevated dpp transcription in ECs. Moreover, ectopic Engrailed (En) expression in osa-depleted ECs partially contributes to upregulated dpp transcription. Furthermore, we show that Osa regulates germline differentiation in a Brahma (Brm)-associated protein (BAP)-complex-dependent manner. Additionally, the loss of EC long cellular processes upon osa depletion may also partly contribute to the germline differentiation defect. Taken together, these data suggest that the epigenetic factor Osa plays an important role in controlling EC characteristics and germline lineage differentiation.

scholarly journals Smad-Independent BMP Signaling in Somatic Cells Limits the Size of the Germline Stem Cell Pool

2018 ◽  
Vol 11 (3) ◽  
pp. 811-827 ◽  
Author(s):  
Chen-Yuan Tseng ◽  
Yu-Han Su ◽  
Shun-Min Yang ◽  
Kun-Yang Lin ◽  
Chun-Ming Lai ◽  
...  
Keyword(s):  
Stem Cell ◽  
Somatic Cells ◽  
Bmp Signaling ◽  
Germline Stem Cell ◽  
Cell Pool ◽  
Stem Cell Pool

scholarly journals magu is required for germline stem cell self-renewal through BMP signaling in the Drosophila testis

2011 ◽  
Vol 357 (1) ◽  
pp. 202-210 ◽  
Author(s):  
Qi Zheng ◽  
Yiwen Wang ◽  
Eric Vargas ◽  
Stephen DiNardo
Keyword(s):  
Stem Cell ◽  
Bmp Signaling ◽  
Germline Stem Cell ◽  
Self Renewal ◽  
Drosophila Testis

scholarly journals The Bric-à-Brac transcription factors are necessary for formation of functional germline stem cell niches through control of dpp expression in the Drosophila melanogaster ovary

2019 ◽  
Author(s):  
Laurine Miscopein Saler ◽  
Mathieu Bartoletti ◽  
Virginie Hauser ◽  
Anne-Marie Pret ◽  
Laurent Theodore ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Stem Cell ◽  
Transcription Factors ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Somatic Cells ◽  
Bmp Signaling ◽  
Germline Stem Cell ◽  
Larval Stages ◽  
Functional Germline

AbstractMany studies have focused on the mechanisms of stem cell maintenance via their interaction with a particular niche or microenvironment in adult tissues, but how formation of a functional niche is initiated, including how stem cells within a niche are established, is less well understood. Adult Drosophila melanogaster ovary Germline Stem Cell (GSC) niches are comprised of somatic cells forming a stack called a Terminal Filament (TF) and underlying Cap Cells (CCs) and Escort Cells (ECs), which are in direct contact with GSCs. In the adult, the Engrailed (En) transcription factor is specifically expressed in niche cells where it directly controls expression of the decapentaplegic gene (dpp) encoding a member of the Bone Morphogenetic Protein (BMP) family of secreted signaling molecules, which are key factors for GSC maintenance. In late third instar larval ovaries, in response to BMP signaling from newly-formed niches, adjacent primordial germ cells become GSCs. The bric-à-brac paralogs (bab1 and bab2) encode BTB/POZ-domain containing transcription factors, that are also expressed in developing GSCs niches where they are required for TF formation. Here, we demonstrate that Bab1 and Bab2 display redundant cell autonomous function for TF morphogenesis and we identify a new function for these genes in GSC establishment. Moreover, we show that Bab proteins control dpp expression in otherwise correctly specified CCs, independently of En and its paralog Invected (Inv). In fact, our results also indicate that en/inv function in larval stages are neither essential for TF formation, nor GSC establishment. Finally, when bab2 was overexpressed in ovarian somatic cells outside of the niche, where en/inv were not expressed, ectopic BMP signaling activation was induced in adjacent germ cells of adult ovaries, which formed GSC-like tumors. Together, these results indicate that Bab transcription factors are positive regulators of BMP signaling for acquisition of GSC status.

scholarly journals Ecdysone signaling promotes expression of multifunctional RNA binding proteins essential for ovarian germline stem cell self-renewal in Drosophila

2018 ◽  
Author(s):  
Danielle S. Finger ◽  
Vivian V. Holt ◽  
Elizabeth T. Ables
Keyword(s):  
Stem Cell ◽  
Molecular Mechanisms ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Bmp Signaling ◽  
Germline Stem Cell ◽  
Self Renewal ◽  
Bmp Receptors ◽  
Morphogenetic Protein ◽  
Ecdysone Signaling

ABSTRACTSteroid hormones promote stem cell self-renewal in many tissues; however, the molecular mechanisms by which hormone signaling is integrated with niche-derived signals are largely uncharacterized. In the Drosophila ovary, the steroid hormone ecdysone promotes germline stem cell (GSC) self-renewal. Despite strong evidence that ecdysone modulates the reception of bone morphogenetic protein (BMP) signals in GSCs, transcriptional targets of ecdysone signaling that facilitate BMP reception are unknown. Here, we report that ecdysone signaling promotes the expression of the heterogeneous nuclear ribonucleoproteins (hnRNPs) squid, hephaestus, Hrb27C, and Hrb87F in GSCs. These hnRNPs functionally interact with ecdysone signaling to control GSC number and are cell autonomously required in GSCs for their maintenance. We demonstrate that hnRNPs promote GSC self-renewal by binding to transcripts essential for proper BMP signaling, including the BMP receptors thickveins and punt. Our findings support the model that stem cells coordinate local and long-range signals at the transcriptional and post-transcriptional levels to maintain self-renewal in response to physiological demand.GRAPHICAL ABSTRACTEcdysone signaling regulates distinct hnRNPs that bind to BMP signaling targets to control GSC self-renewal.SUMMARY STATEMENTEcdysone signaling promotes expression of heterogeneous ribonucleoproteins that modulate BMP-dependent germline stem cell self-renewal in the Drosophila ovary.

scholarly journals Dlp-mediated Hh and Wnt signaling interdependence is critical in the niche for germline stem cell progeny differentiation

2020 ◽  
Vol 6 (20) ◽  
pp. eaaz0480 ◽  
Author(s):  
Renjun Tu ◽  
Bo Duan ◽  
Xiaoqing Song ◽  
Ting Xie
Keyword(s):  
Transcription Factor ◽  
Stem Cell ◽  
Wnt Signaling ◽  
Regulatory Mechanism ◽  
Regulatory Region ◽  
Bmp Signaling ◽  
Germline Stem Cell ◽  
Self Renewal ◽  
Wnt Pathways ◽  
Multiple Signaling

Although multiple signaling pathways work synergistically in various niches to control stem cell self-renewal and differentiation, it remains poorly understood how they cooperate with one another molecularly. In the Drosophila ovary, Hh and Wnt pathways function in the niche to promote germline stem cell (GSC) progeny differentiation. Here, we show that glypican Dlp-mediated Hh and Wnt signaling interdependence operates in the niche to promote GSC progeny differentiation by preventing BMP signaling. Hh/Wnt-mediated dlp repression is essential for their signaling interdependence in niche cells and for GSC progeny differentiation by preventing BMP signaling. Mechanistically, Hh and Wnt downstream transcription factors directly bind to the same dlp regulatory region and recruit corepressors composed of transcription factor Croc and Egg/H3K9 trimethylase to repress Dlp expression. Therefore, our study reveals a novel mechanism for Hh/Wnt signaling–mediated direct dlp repression and a novel regulatory mechanism for Dlp-mediated Hh/Wnt signaling interdependence in the GSC differentiation niche.

scholarly journals The Bric-à-Brac BTB/POZ transcription factors are necessary in niche cells for germline stem cells establishment and homeostasis through control of BMP/DPP signaling in the Drosophila melanogaster ovary

PLoS Genetics ◽  
2020 ◽  
Vol 16 (11) ◽  
pp. e1009128
Author(s):  
Laurine Miscopein Saler ◽  
Virginie Hauser ◽  
Mathieu Bartoletti ◽  
Charlotte Mallart ◽  
Marianne Malartre ◽  
...  
Keyword(s):  
Stem Cells ◽  
Drosophila Melanogaster ◽  
Stem Cell ◽  
Transcription Factors ◽  
Germ Cells ◽  
Somatic Cells ◽  
Bmp Signaling ◽  
Germline Stem Cell ◽  
Larval Stages ◽  
Adult Ovary

Many studies have focused on the mechanisms of stem cell maintenance via their interaction with a particular niche or microenvironment in adult tissues, but how formation of a functional niche is initiated, including how stem cells within a niche are established, is less well understood. Adult Drosophila melanogaster ovary Germline Stem Cell (GSC) niches are comprised of somatic cells forming a stack called a Terminal Filament (TF) and associated Cap and Escort Cells (CCs and ECs, respectively), which are in direct contact with GSCs. In the adult ovary, the transcription factor Engrailed is specifically expressed in niche cells where it directly controls expression of the decapentaplegic (dpp) gene encoding a member of the Bone Morphogenetic Protein (BMP) family of secreted signaling molecules, which are key factors for GSC maintenance. In larval ovaries, in response to BMP signaling from newly formed niches, adjacent primordial germ cells become GSCs. The bric-à-brac paralogs (bab1 and bab2) encode BTB/POZ domain-containing transcription factors that are expressed in developing niches of larval ovaries. We show here that their functions are necessary specifically within precursor cells for TF formation during these stages. We also identify a new function for Bab1 and Bab2 within developing niches for GSC establishment in the larval ovary and for robust GSC maintenance in the adult. Moreover, we show that the presence of Bab proteins in niche cells is necessary for activation of transgenes reporting dpp expression as of larval stages in otherwise correctly specified Cap Cells, independently of Engrailed and its paralog Invected (En/Inv). Moreover, strong reduction of engrailed/invected expression during larval stages does not impair TF formation and only partially reduces GSC numbers. In the adult ovary, Bab proteins are also required for dpp reporter expression in CCs. Finally, when bab2 was overexpressed at this stage in somatic cells outside of the niche, there were no detectable levels of ectopic En/Inv, but ectopic expression of a dpp transgene was found in these cells and BMP signaling activation was induced in adjacent germ cells, which produced GSC-like tumors. Together, these results indicate that Bab transcription factors are positive regulators of BMP signaling in niche cells for establishment and homeostasis of GSCs in the Drosophila ovary.

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