scholarly journals Multicolor Cell Barcoding Technology for Long-Term Surveillance of Epithelial Regeneration in Zebrafish

2016 ◽  
Vol 36 (6) ◽  
pp. 668-680 ◽  
Author(s):  
Chen-Hui Chen ◽  
Alberto Puliafito ◽  
Ben D. Cox ◽  
Luca Primo ◽  
Yi Fang ◽  
...  
Keyword(s):  
Epithelial Regeneration ◽  
Barcoding Technology

Epithelial Regeneration and Wound Healing in the Oesophagus of the Cat

Development ◽  
1958 ◽  
Vol 6 (2) ◽  
pp. 288-296
Author(s):  
R. M. H. McMinn ◽  
F. R. Johnson
Keyword(s):  
Wound Healing ◽  
Connective Tissue ◽  
Experimental Work ◽  
Experimental Studies ◽  
Long Term Results ◽  
Muscular Tissue ◽  
Small Areas ◽  
Epithelial Regeneration ◽  
Recent Experimental Work

Saint in 1929 documented the experimental studies that had been carried out up to that date on the healing of oesophageal wounds. The reported observations dealt with the relatively long-term results, and little attention was paid to the early changes that occurred during the period immediately following the infliction of the wound. Recently, Malm (1951) made observations following surgical experiments on the oesophagus in dogs, but again no reference was made to the early changes in epithelium or connective tissue. The most recent experimental work on oesophageal healing appears to be that of Picard, Henry, Cotte, & Inglesakis (1956), but their interest lay in the repair of muscular tissue. The present investigation was designed to study the behaviour of epithelium and connective tissue in the cat's oesophagus, following the removal of small areas of mucous membrane. Routine histological methods were supplemented by a series of histochemical studies on both the normal and regenerating tissues.

scholarly journals Stable Long-Term Culture of Human Distal Airway Stem Cells for Transplantation

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Yueqing Zhou ◽  
Yujia Wang ◽  
Dandan Li ◽  
Ting Zhang ◽  
Yu Ma ◽  
...  
Keyword(s):  
Stem Cells ◽  
Lung Model ◽  
Mouse Lung ◽  
Secretory Cells ◽  
Type I ◽  
Alveolar Cells ◽  
Epithelial Regeneration ◽  
Distal Airway ◽  
Promising Therapeutic Approach

There is a population of p63+/Krt5+ distal airway stem cells (DASCs) quiescently located in the airway basal epithelium of mammals, responding to injury and airway epithelial regeneration. They hold the ability to differentiate into multiple pulmonary cell types and can repopulate the epithelium after damage. The current study aims at gaining further insights into the behavior and characteristics of the DASCs isolated from the patient lung and exploring their clinical translational potential. Human DASCs were brushed off through the bronchoscopic procedure and expanded under the pharmaceutical-grade condition. Their phenotype stability in long-term cell culture was analyzed, followed by safety evaluation and tumorigenic analysis using multiple animal models including rodents and nonhuman primate. The chimerism of the human-mouse lung model indicated that DASC pedigrees could give rise to multiple epithelial types, including type I alveolar cells as well as bronchiolar secretory cells, to regenerate the distal lung. Taken together, the results suggested that DASC transplantation could be a promising therapeutic approach for unmet needs in respiratory medicine including the COVID-19-related diseases.

scholarly journals Interleukin 22 prevents lipopolysaccharide- induced preterm labor in mice†

2018 ◽  
Vol 98 (3) ◽  
pp. 299-308 ◽  
Author(s):  
Svetlana Dambaeva ◽  
Sylvia Schneiderman ◽  
Mukesh K Jaiswal ◽  
Varkha Agrawal ◽  
Gajendra K Katara ◽  
...  
Keyword(s):  
Preterm Birth ◽  
Preterm Labor ◽  
Wound Repair ◽  
Potential Role ◽  
Treatment Method ◽  
Neonatal Deaths ◽  
Interleukin 22 ◽  
Epithelial Regeneration

Abstract Preterm birth is widespread and causes 35% of all neonatal deaths. Infants who survive face potential long-term complications. A major contributing factor of preterm birth is infection. We investigated the role of interleukin 22 (IL22) as a potential clinically relevant cytokine during gestational infection. IL22 is an effector molecule secreted by immune cells. While the expression of IL22 was reported in normal nonpregnant endometrium and early pregnancy decidua, little is known about uterine IL22 expression during mid or late gestational stages of pregnancy. Since IL22 has been shown to be an essential mediator in epithelial regeneration and wound repair, we investigated the potential role of IL22 during defense against an inflammatory response at the maternal–fetal interface. We used a well-established model to study infection and infection-associated inflammation during preterm birth in the mouse. We have shown that IL22 is upregulated to respond to an intrauterine lipopolysaccharide administration and plays an important role in controlling the risk of inflammation-induced preterm birth. This paper proposes IL22 as a treatment method to combat infection and prevent preterm birth in susceptible patients.

scholarly journals Long-term effect of parasympathetic or sympathetic denervation on intestinal epithelial cell proliferation and apoptosis

2017 ◽  
Vol 242 (15) ◽  
pp. 1499-1507 ◽  
Author(s):  
Elizabeth A Davis ◽  
Martha C Washington ◽  
Emily R Yaniz ◽  
Heidi Phillips ◽  
Ayman I Sayegh ◽  
...  
Keyword(s):  
Food Intake ◽  
Cell Number ◽  
Term Effect ◽  
Sympathetic Denervation ◽  
Intestinal Epithelial ◽  
Epithelial Regeneration ◽  
Sham Surgery ◽  
Long Term Effect ◽  
Induced Changes

Intestinal epithelial tissue is constantly regenerated as a means to maintain proper tissue function. Previous studies have demonstrated that denervation of the parasympathetic or sympathetic nervous system to the intestine alters this process. However, results are inconsistent between studies, showing both increases and decreases in proliferation after denervation of the parasympathetic or sympathetic. The effect appears to correlate with (1) the timing post-denervation, (2) denervation-induced changes in food intake, (3) the denervation technique used, and (4) which intestinal segment is investigated. Thus, we proposed that parasympathetic or sympathetic denervation does not have an effect on intestinal epithelial regeneration when you (1) evaluate denervation after long-term denervation, (2) control for post-surgical changes in food intake, (3) use minimally invasive surgical techniques and (4) include a segmental analysis. To test this, adult male Sprague Dawley rats underwent parasympathetic denervation via subdiaphragmatic vagotomy, sympathetic denervation via celiacomesenteric ganglionectomy, a parasympathetic denervation sham surgery, or a sympathetic denervation sham surgery. Sham surgery ad libitum-fed groups and sham surgery pair-fed groups were used to control for surgically induced changes in food intake. Three weeks post-surgery, animals were sacrificed and tissue from the duodenum, jejunum, and ileum was excised and immunohistochemically processed to visualize indicators of proliferation (bromodeoxyuridine-positive cells) and apoptosis (caspase-3-positive cells). Results showed no differences between groups in proliferation, apoptosis, or total cell number in any intestinal segment. These results suggest that parasympathetic or sympathetic denervation does not have a significant long-term effect on intestinal epithelial turnover. Thus, intestinal epithelial regeneration is able to recover after autonomic nervous system injury. Impact statement This study investigates the long-term effect of autonomic denervation on intestinal epithelial cell turnover, as measured by proliferation, apoptosis, and total cell number. Although previous research has established that autonomic denervation can alter intestinal epithelial turnover under short-term conditions, here we establish for the first time that these changes do not persist long-term when you control for surgical-induced changes in food intake and use targeted denervation procedures. These findings add to the base of knowledge on autonomic control of tissue turnover, highlight the ability of the intestinal epithelium to recover after autonomic injury and reveal possible implications of the use of ANS denervation for disease treatment in humans.

Therapy and prevention for mental health: What if mental diseases are mostly not brain disorders?

2019 ◽  
Vol 42 ◽  
Author(s):  
John P. A. Ioannidis
Keyword(s):  
Mental Health ◽  
Mental Disease ◽  
Brain Disorders ◽  
Social Stressors ◽  
Labor Education ◽  
Mental Diseases ◽  
Long Term Follow Up ◽  
Political Decisions

AbstractNeurobiology-based interventions for mental diseases and searches for useful biomarkers of treatment response have largely failed. Clinical trials should assess interventions related to environmental and social stressors, with long-term follow-up; social rather than biological endpoints; personalized outcomes; and suitable cluster, adaptive, and n-of-1 designs. Labor, education, financial, and other social/political decisions should be evaluated for their impacts on mental disease.

Conceptual short-term memory (CSTM) supports core claims of Christiansen and Chater

2016 ◽  
Vol 39 ◽  
Author(s):  
Mary C. Potter
Keyword(s):  
Working Memory ◽  
Rapid Serial Visual Presentation ◽  
Language Comprehension ◽  
Short Term Memory ◽  
Visual Presentation ◽  
Long Term Memory ◽  
Short Term ◽  
Term Memory ◽  
Use Of Knowledge

AbstractRapid serial visual presentation (RSVP) of words or pictured scenes provides evidence for a large-capacity conceptual short-term memory (CSTM) that momentarily provides rich associated material from long-term memory, permitting rapid chunking (Potter 1993; 2009; 2012). In perception of scenes as well as language comprehension, we make use of knowledge that briefly exceeds the supposed limits of working memory.

Brief summary of Kleť photographic search programme for minor planets

1999 ◽  
Vol 173 ◽  
pp. 189-192
Author(s):  
J. Tichá ◽  
M. Tichý ◽  
Z. Moravec
Keyword(s):  
Ccd Camera ◽  
Minor Planet ◽  
Minor Planets ◽  
Main Belt

AbstractA long-term photographic search programme for minor planets was begun at the Kleť Observatory at the end of seventies using a 0.63-m Maksutov telescope, but with insufficient respect for long-arc follow-up astrometry. More than two thousand provisional designations were given to new Kleť discoveries. Since 1993 targeted follow-up astrometry of Kleť candidates has been performed with a 0.57-m reflector equipped with a CCD camera, and reliable orbits for many previous Kleť discoveries have been determined. The photographic programme results in more than 350 numbered minor planets credited to Kleť, one of the world's most prolific discovery sites. Nearly 50 per cent of them were numbered as a consequence of CCD follow-up observations since 1994.This brief summary describes the results of this Kleť photographic minor planet survey between 1977 and 1996. The majority of the Kleť photographic discoveries are main belt asteroids, but two Amor type asteroids and one Trojan have been found.

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