scholarly journals Zygotic Genome Activation Triggers Chromosome Damage and Checkpoint Signaling in C. elegans Primordial Germ Cells

2015 ◽  
Vol 34 (1) ◽  
pp. 85-95 ◽  
Author(s):  
Melina Butuči ◽  
Ashley B. Williams ◽  
Matthew M. Wong ◽  
Brendan Kramer ◽  
W. Matthew Michael
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Chromosome Damage ◽  
Zygotic Genome Activation ◽  
Checkpoint Signaling ◽  
C Elegans ◽  
Genome Activation ◽  
Zygotic Genome

scholarly journals Whole-Transcriptome Sequencing-Based Analysis of DAZL and Its Interacting Genes during Germ Cells Specification and Zygotic Genome Activation in Chickens

2020 ◽  
Vol 21 (21) ◽  
pp. 8170
Author(s):  
Deivendran Rengaraj ◽  
Sohyoung Won ◽  
Jong Won Han ◽  
DongAhn Yoo ◽  
Heebal Kim ◽  
...  
Keyword(s):  
Germ Cells ◽  
Transcriptome Sequencing ◽  
Germ Plasm ◽  
Prediction Method ◽  
Zygotic Genome Activation ◽  
Zygotic Transcription ◽  
Whole Transcriptome Sequencing ◽  
Genome Activation ◽  
Whole Transcriptome ◽  
Zygotic Genome

The deleted in azoospermia like (DAZL) is required for germ cells development and maintenance. In chickens, the mRNA and protein of DAZL, a representative maternally inherited germ plasm factor, are detected in the germ plasm of oocyte, zygote, and all stages of the intrauterine embryos. However, it is still insufficient to explain the origin and specification process of chicken germ cells, because the stage at which the zygotic transcription of DAZL occurs and the stage at which the maternal DAZL RNA/protein clears have not yet been fully identified. Moreover, a comprehensive understanding of the expression of DAZL interacting genes during the germ cells specification and development and zygotic genome activation (ZGA) is lacking in chickens. In this study, we identified a set of DAZL interacting genes in chickens using in silico prediction method. Then, we analyzed the whole-transcriptome sequencing (WTS)-based expression of DAZL and its interacting genes in the chicken oocyte, zygote, and Eyal-Giladi and Kochav (EGK) stage embryos (EGK.I to EGK.X). In the results, DAZL transcripts are increased in the zygote (onset of transcription), maintained the increased level until EGK.VI, and decreased from EGK.VIII (possible clearance of maternal RNAs). Among the DAZL interacting genes, most of them are increased either at 1st ZGA or 2nd ZGA, indicating their involvement in germ cells specification and development.

CLAMP regulates Zygotic Genome Activation in Drosophila embryos

Genetics ◽  
2021 ◽  
Author(s):  
Megan M Colonnetta ◽  
Juan E Abrahante ◽  
Paul Schedl ◽  
Daryl M Gohl ◽  
Girish Deshpande
Keyword(s):  
Temporal Dynamics ◽  
Embryonic Patterning ◽  
Zygotic Genome Activation ◽  
Promoter Sequences ◽  
Genome Wide ◽  
Zygotic Transcription ◽  
Pioneer Factor ◽  
Genome Activation ◽  
Drosophila Embryos ◽  
Zygotic Genome

Abstract Embryonic patterning is critically dependent on zygotic genome activation (ZGA). In Drosophila melanogaster embryos, the pioneer factor Zelda directs ZGA, possibly in conjunction with other factors. Here we have explored novel involvement of Chromatin-Linked Adapter for MSL Proteins (CLAMP) during ZGA. CLAMP binds thousands of sites genome-wide throughout early embryogenesis. Interestingly, CLAMP relocates to target promoter sequences across the genome when ZGA is initiated. Although there is a considerable overlap between CLAMP and Zelda binding sites, the proteins display distinct temporal dynamics. To assess whether CLAMP occupancy affects gene expression, we analyzed transcriptomes of embryos zygotically compromised for either clamp or zelda and found that transcript levels of many zygotically-activated genes are similarly affected. Importantly, compromising either clamp or zelda disrupted the expression of critical segmentation and sex determination genes bound by CLAMP (and Zelda). Furthermore, clamp knockdown embryos recapitulate other phenotypes observed in Zelda-depleted embryos, including nuclear division defects, centrosome aberrations, and a disorganized actomyosin network. Based on these data, we propose that CLAMP acts in concert with Zelda to regulate early zygotic transcription.

scholarly journals Zygotic Genome Activation in Vertebrates

2017 ◽  
Vol 42 (4) ◽  
pp. 316-332 ◽  
Author(s):  
David Jukam ◽  
S. Ali M. Shariati ◽  
Jan M. Skotheim
Keyword(s):  
Zygotic Genome Activation ◽  
Genome Activation ◽  
Zygotic Genome

MouseXist expression begins at zygotic genome activation and is timed by a zygotic clock

2001 ◽  
Vol 61 (1) ◽  
pp. 14-20 ◽  
Author(s):  
Maurizio Zuccotti ◽  
Michele Boiani ◽  
Ruben Ponce ◽  
Stefano Guizzardi ◽  
Renato Scandroglio ◽  
...  
Keyword(s):  
Zygotic Genome Activation ◽  
Genome Activation ◽  
Zygotic Genome

scholarly journals Endodermal Maternal Transcription Factors Establish Super-Enhancers during Zygotic Genome Activation

Cell Reports ◽  
2019 ◽  
Vol 27 (10) ◽  
pp. 2962-2977.e5 ◽  
Author(s):  
Kitt D. Paraiso ◽  
Ira L. Blitz ◽  
Masani Coley ◽  
Jessica Cheung ◽  
Norihiro Sudou ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Zygotic Genome Activation ◽  
Genome Activation ◽  
Zygotic Genome
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