scholarly journals S1P-Yap1 Signaling Regulates Endoderm Formation Required for Cardiac Precursor Cell Migration in Zebrafish

2014 ◽  
Vol 31 (1) ◽  
pp. 128-136 ◽  
Author(s):  
Hajime Fukui ◽  
Kenta Terai ◽  
Hiroyuki Nakajima ◽  
Ayano Chiba ◽  
Shigetomo Fukuhara ◽  
...  
Keyword(s):  
Cell Migration ◽  
Precursor Cell

scholarly journals Developmental Neurotoxicity of Fipronil and Rotenone on a Human Neuronal In Vitro Test System

2021 ◽  
Author(s):  
Anne Schmitz ◽  
Silke Dempewolf ◽  
Saime Tan ◽  
Gerd Bicker ◽  
Michael Stern
Keyword(s):  
Cell Migration ◽  
Neurite Outgrowth ◽  
Neuronal Differentiation ◽  
Precursor Cell ◽  
Developmental Neurotoxicity ◽  
In Vitro Studies ◽  
Human Model ◽  
In Vitro Test ◽  
Dependent Manner

AbstractPesticide exposure during in utero and early postnatal development can cause a wide range of neurological defects. However, relatively few insecticides have been recognized as developmental neurotoxicants, so far. Recently, discovery of the insecticide, fipronil, in chicken eggs has raised public concern. The status of fipronil as a potential developmental neurotoxicant is still under debate. Whereas several in vivo and in vitro studies suggest specific toxicity, other in vitro studies could not confirm this concern. Here, we tested fipronil and its main metabolic product, fipronil sulfone both at concentrations between 1.98 and 62.5 µM, alongside with the established developmental neurotoxicant, rotenone (0.004–10 µM) in vitro on the human neuronal precursor cell line NT2. We found that rotenone impaired all three tested DNT endpoints, neurite outgrowth, neuronal differentiation, and precursor cell migration in a dose-dependent manner and clearly separable from general cytotoxicity in the nanomolar range. Fipronil and fipronil sulfone specifically inhibited cell migration and neuronal differentiation, but not neurite outgrowth in the micromolar range. The rho-kinase inhibitor Y-27632 counteracted inhibition of migration for all three compounds (EC50 between 12 and 50 µM). The antioxidant, n-acetyl cysteine, could ameliorate the inhibitory effects of fipronil on all three tested endpoints (EC 50 between 84 and 164 µM), indicating the involvement of oxidative stress. Fipronil sulfone had a stronger effect than fipronil, confirming the importance to test metabolic products alongside original pesticides. We conclude that in vitro fipronil and fipronil sulfone display specific developmental neurotoxicity on developing human model neurons.

The Rho Kinase Pathway Regulates Mouse Adult Neural Precursor Cell Migration

Stem Cells ◽  
2011 ◽  
Vol 29 (2) ◽  
pp. 332-343 ◽  
Author(s):  
Soo Yuen Leong ◽  
Clare H. Faux ◽  
Alisa Turbic ◽  
Kirsty J. Dixon ◽  
Ann M. Turnley
Keyword(s):  
Cell Migration ◽  
Rho Kinase ◽  
Precursor Cell ◽  
Neural Precursor ◽  
Neural Precursor Cell ◽  
Kinase Pathway

scholarly journals Full-Length and Fragmented Netrin-1 in Multiple Sclerosis Plaques Are Inhibitors of Oligodendrocyte Precursor Cell Migration

2013 ◽  
Vol 183 (3) ◽  
pp. 673-680 ◽  
Author(s):  
Jenea M. Bin ◽  
Sathyanath Rajasekharan ◽  
Tanja Kuhlmann ◽  
Ilana Hanes ◽  
Nathalie Marcal ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Cell Migration ◽  
Precursor Cell ◽  
Full Length ◽  
Oligodendrocyte Precursor Cell ◽  
Oligodendrocyte Precursor

Contrasting effects of mitogenic growth factors on oligodendrocyte precursor cell migration

Glia ◽  
1997 ◽  
Vol 19 (1) ◽  
pp. 85-90 ◽  
Author(s):  
Richard Milner ◽  
Helen J. Anderson ◽  
Rebecca F. Rippon ◽  
Jennifer S. McKay ◽  
Robin J.M. Franklin ◽  
...  
Keyword(s):  
Cell Migration ◽  
Growth Factors ◽  
Precursor Cell ◽  
Oligodendrocyte Precursor Cell ◽  
Oligodendrocyte Precursor

scholarly journals Paracrine Pax6 activity regulates oligodendrocyte precursor cell migration in the chick embryonic neural tube

Development ◽  
2011 ◽  
Vol 138 (22) ◽  
pp. 4991-5001 ◽  
Author(s):  
E. Di Lullo ◽  
C. Haton ◽  
C. Le Poupon ◽  
M. Volovitch ◽  
A. Joliot ◽  
...  
Keyword(s):  
Cell Migration ◽  
Neural Tube ◽  
Precursor Cell ◽  
Oligodendrocyte Precursor Cell ◽  
Oligodendrocyte Precursor

A brain slice culture model for studies of endogenous and exogenous precursor cell migration in the rostral migratory stream

2009 ◽  
Vol 1295 ◽  
pp. 1-12 ◽  
Author(s):  
Mette Tanvig ◽  
Morten Blaabjerg ◽  
Rikke K. Andersen ◽  
Ana Villa ◽  
Ann Mari Rosager ◽  
...  
Keyword(s):  
Cell Migration ◽  
Brain Slice ◽  
Rostral Migratory Stream ◽  
Precursor Cell ◽  
Slice Culture ◽  
Culture Model ◽  
Brain Slice Culture ◽  
Migratory Stream

EphA4/ephrin-A5 interactions in muscle precursor cell migration in the avian forelimb

Development ◽  
2001 ◽  
Vol 128 (23) ◽  
pp. 4669-4680 ◽  
Author(s):  
Mary E. Swartz ◽  
Johann Eberhart ◽  
Elena B. Pasquale ◽  
Catherine E. Krull
Keyword(s):  
Cell Migration ◽  
Precursor Cells ◽  
Precursor Cell ◽  
Specific Gene ◽  
In Ovo ◽  
Muscle Precursor ◽  
In Ovo Electroporation ◽  
Lateral Portion ◽  
Muscle Precursor Cells ◽  
Muscle Precursor Cell

Limb muscles derive from muscle precursor cells that lie initially in the lateral portion of the somitic dermomyotome and subsequently migrate to their target limb regions, where muscle-specific gene transcription is initiated. Although several molecules that control the generation and delamination of muscle precursor cells have been identified, little is known about the mechanisms that guide muscle precursor cell migration in the limb. We have examined the distribution of members of the Eph family during muscle precursor cell development. The EphA4 receptor tyrosine kinase and its ligand, ephrin-A5, are expressed by muscle precursor cells and forelimb mesoderm in unique spatiotemporal patterns during the period when muscle precursors delaminate from the dermomyotome and migrate into the limb. To test the function of EphA4/ephrin-A5 interactions in muscle precursor migration, we used targeted in ovo electroporation to express ephrin-A5 ectopically specifically in the presumptive limb mesoderm. In the presence of ectopic ephrin-A5, Pax7-positive muscle precursor cells are significantly reduced in number in the proximal limb, compared with controls, and congregate abnormally near the lateral dermomyotome. In stripe assays, isolated muscle precursor cells avoid substrate-bound ephrin-A5 and this avoidance is abolished by addition of soluble ephrin-A5. These data suggest that ephrin-A5 normally restricts migrating, EphA4-positive muscle precursor cells to their appropriate territories in the forelimb, disallowing entry into abnormal embryonic regions.

Sign in / Sign up

Export Citation Format

Share Document