Recent advances in production of recombinant spider silk proteins

2012 ◽  
Vol 23 (6) ◽  
pp. 957-964 ◽  
Author(s):  
Hannah Chung ◽  
Tae Yong Kim ◽  
Sang Yup Lee
Keyword(s):  
Spider Silk ◽  
Silk Proteins ◽  
Recent Advances

scholarly journals Designing of spider silk proteins for hiPSC-based cardiac tissue engineering

2021 ◽  
pp. 100114
Author(s):  
Tilman U. Esser ◽  
Vanessa T. Trossmann ◽  
Sarah Lentz ◽  
Felix B. Engel ◽  
Thomas Scheibel
Keyword(s):  
Tissue Engineering ◽  
Spider Silk ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering ◽  
Silk Proteins

Utilizing Conformational Changes for Patterning Thin Films of Recombinant Spider Silk Proteins

2012 ◽  
Vol 13 (10) ◽  
pp. 3189-3199 ◽  
Author(s):  
Seth L. Young ◽  
Maneesh Gupta ◽  
Christoph Hanske ◽  
Andreas Fery ◽  
Thomas Scheibel ◽  
...  
Keyword(s):  
Thin Films ◽  
Conformational Changes ◽  
Spider Silk ◽  
Silk Proteins

Cell adhesion and proliferation on RGD-modified recombinant spider silk proteins

Biomaterials ◽  
2012 ◽  
Vol 33 (28) ◽  
pp. 6650-6659 ◽  
Author(s):  
Stefanie Wohlrab ◽  
Susanne Müller ◽  
Andreas Schmidt ◽  
Stefanie Neubauer ◽  
Horst Kessler ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Spider Silk ◽  
Silk Proteins ◽  
Cell Adhesion And Proliferation

scholarly journals Recombinant spider silk proteins as example for supramolecular printable hydrogels

2016 ◽  
Vol 4 ◽  
Author(s):  
J�ngst Tomasz ◽  
Schacht Kristin ◽  
Smolan Willi ◽  
Ewald Andrea ◽  
Scheibel Thomas ◽  
...  
Keyword(s):  
Spider Silk ◽  
Silk Proteins

scholarly journals Utilizing Recombinant Spider Silk Proteins To Develop a Synthetic Bruch’s Membrane for Modeling the Retinal Pigment Epithelium

2019 ◽  
Vol 5 (8) ◽  
pp. 4023-4036 ◽  
Author(s):  
Thomas I. Harris ◽  
Chase A. Paterson ◽  
Farhad Farjood ◽  
Ian D. Wadsworth ◽  
Lori Caldwell ◽  
...  
Keyword(s):  
Retinal Pigment Epithelium ◽  
Spider Silk ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Bruch's Membrane ◽  
Bruch’S Membrane ◽  
Silk Proteins

scholarly journals Properties of Biomimetic Artificial Spider Silk Fibers Tuned by PostSpin Bath Incubation

Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3248
Author(s):  
Gabriele Greco ◽  
Juanita Francis ◽  
Tina Arndt ◽  
Benjamin Schmuck ◽  
Fredrik G. Bäcklund ◽  
...  
Keyword(s):  
Spider Silk ◽  
Molecular Mechanisms ◽  
Efficient Production ◽  
Fiber Morphology ◽  
Silk Proteins ◽  
Silk Fibers ◽  
Aqueous Environments ◽  
Natural Counterpart ◽  
Phosphate Buffered Saline ◽  
Β Sheet

Efficient production of artificial spider silk fibers with properties that match its natural counterpart has still not been achieved. Recently, a biomimetic process for spinning recombinant spider silk proteins (spidroins) was presented, in which important molecular mechanisms involved in native spider silk spinning were recapitulated. However, drawbacks of these fibers included inferior mechanical properties and problems with low resistance to aqueous environments. In this work, we show that ≥5 h incubation of the fibers, in a collection bath of 500 mM NaAc and 200 mM NaCl, at pH 5 results in fibers that do not dissolve in water or phosphate buffered saline, which implies that the fibers can be used for applications that involve wet/humid conditions. Furthermore, incubation in the collection bath improved the strain at break and was associated with increased β-sheet content, but did not affect the fiber morphology. In summary, we present a simple way to improve artificial spider silk fiber strain at break and resistance to aqueous solvents.

A bilayered scaffold based on RGD recombinant spider silk proteins for small diameter tissue engineering

2014 ◽  
Vol 37 (2) ◽  
pp. 523-531 ◽  
Author(s):  
Chao-ying Zhang ◽  
Da-chun Zhang ◽  
Deng-long Chen ◽  
Min Li
Keyword(s):  
Tissue Engineering ◽  
Spider Silk ◽  
Small Diameter ◽  
Silk Proteins
Sign in / Sign up

Export Citation Format

Share Document