A bilayered scaffold based on RGD recombinant spider silk proteins for small diameter tissue engineering

2014 ◽  
Vol 37 (2) ◽  
pp. 523-531 ◽  
Author(s):  
Chao-ying Zhang ◽  
Da-chun Zhang ◽  
Deng-long Chen ◽  
Min Li
Keyword(s):  
Tissue Engineering ◽  
Spider Silk ◽  
Small Diameter ◽  
Silk Proteins

scholarly journals Designing of spider silk proteins for hiPSC-based cardiac tissue engineering

2021 ◽  
pp. 100114
Author(s):  
Tilman U. Esser ◽  
Vanessa T. Trossmann ◽  
Sarah Lentz ◽  
Felix B. Engel ◽  
Thomas Scheibel
Keyword(s):  
Tissue Engineering ◽  
Spider Silk ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering ◽  
Silk Proteins

scholarly journals Spider Silk for Tissue Engineering Applications

Molecules ◽  
2020 ◽  
Vol 25 (3) ◽  
pp. 737 ◽  
Author(s):  
Sahar Salehi ◽  
Kim Koeck ◽  
Thomas Scheibel
Keyword(s):  
Tissue Engineering ◽  
Spider Silk ◽  
18Th Century ◽  
Three Dimensional ◽  
Silk Proteins ◽  
Natural Production ◽  
Recombinant Production ◽  
Natural Biomaterial

Due to its properties, such as biodegradability, low density, excellent biocompatibility and unique mechanics, spider silk has been used as a natural biomaterial for a myriad of applications. First clinical applications of spider silk as suture material go back to the 18th century. Nowadays, since natural production using spiders is limited due to problems with farming spiders, recombinant production of spider silk proteins seems to be the best way to produce material in sufficient quantities. The availability of recombinantly produced spider silk proteins, as well as their good processability has opened the path towards modern biomedical applications. Here, we highlight the research on spider silk-based materials in the field of tissue engineering and summarize various two-dimensional (2D) and three-dimensional (3D) scaffolds made of spider silk. Finally, different applications of spider silk-based materials are reviewed in the field of tissue engineering in vitro and in vivo.

Biofabrication of 3D constructs: fabrication technologies and spider silk proteins as bioinks

2015 ◽  
Vol 87 (8) ◽  
pp. 737-749 ◽  
Author(s):  
Elise DeSimone ◽  
Kristin Schacht ◽  
Tomasz Jungst ◽  
Jürgen Groll ◽  
Thomas Scheibel
Keyword(s):  
Tissue Engineering ◽  
Self Assembly ◽  
Spider Silk ◽  
High Spatial Resolution ◽  
3D Bioprinting ◽  
High Cell ◽  
Poor Control ◽  
Silk Proteins ◽  
The Past ◽  
Simultaneous Processing

AbstractDespite significant investment in tissue engineering over the past 20 years, few tissue engineered products have made it to market. One of the reasons is the poor control over the 3D arrangement of the scaffold’s components. Biofabrication is a new field of research that exploits 3D printing technologies with high spatial resolution for the simultaneous processing of cells and biomaterials into 3D constructs suitable for tissue engineering. Cell-encapsulating biomaterials used in 3D bioprinting are referred to as bioinks. This review consists of: (1) an introduction of biofabrication, (2) an introduction of 3D bioprinting, (3) the requirements of bioinks, (4) existing bioinks, and (5) a specific example of a recombinant spider silk bioink. The recombinant spider silk bioink will be used as an example because its unmodified hydrogel format fits the basic requirements of bioinks: to be printable and at the same time cytocompatible. The bioink exhibited both cytocompatible (self-assembly, high cell viability) and printable (injectable, shear-thinning, high shape fidelity) qualities. Although improvements can be made, it is clear from this system that, with the appropriate bioink, many of the existing faults in tissue-like structures produced by 3D bioprinting can be minimized.

Utilizing Conformational Changes for Patterning Thin Films of Recombinant Spider Silk Proteins

2012 ◽  
Vol 13 (10) ◽  
pp. 3189-3199 ◽  
Author(s):  
Seth L. Young ◽  
Maneesh Gupta ◽  
Christoph Hanske ◽  
Andreas Fery ◽  
Thomas Scheibel ◽  
...  
Keyword(s):  
Thin Films ◽  
Conformational Changes ◽  
Spider Silk ◽  
Silk Proteins

Spidroin Striped Micropattern Promotes Chondrogenic Differentiation of Human Wharton’s Jelly Mesenchymal Stem Cells

2021 ◽  
Author(s):  
Anggraini Barlian ◽  
Dinda Hani’ah Arum Saputri ◽  
Adriel Hernando ◽  
Ekavianty Prajatelistia ◽  
Hutomo Tanoto
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Chondrogenic Differentiation ◽  
Spider Silk ◽  
Cartilage Tissue Engineering ◽  
Wharton’S Jelly ◽  
Cartilage Tissue ◽  
Type Ii ◽  
Wharton's Jelly

Abstract Cartilage tissue engineering, particularly micropattern, can influence the biophysical properties of mesenchymal stem cells (MSCs) leading to chondrogenesis. In this research, human Wharton’s jelly MSCs (hWJ-MSCs) were grown on a striped micropattern containing spider silk protein (spidroin) from Argiope appensa. This research aims to direct hWJ-MSCs chondrogenesis using micropattern made of spidroin bioink as opposed to fibronectin that often used as the gold standard. Cells were cultured on striped micropattern of 500 µm and 1000 µm width sizes without chondrogenic differentiation medium for 21 days. The immunocytochemistry result showed that spidroin contains RGD sequences and facilitates cell adhesion via integrin β1. Chondrogenesis was observed through the expression of glycosaminoglycan, type II collagen, and SOX9. The result on glycosaminoglycan content proved that 1000 µm was the optimal width to support chondrogenesis. Spidroin micropattern induced significantly higher expression of SOX9 mRNA on day-21 and SOX9 protein was located inside the nucleus starting from day-7. COL2A1 mRNA of spidroin micropattern groups was downregulated on day-21 and collagen type II protein was detected starting from day-14. These results showed that spidroin micropattern enhances chondrogenic markers while maintains long-term upregulation of SOX9, and therefore has the potential as a new method for cartilage tissue engineering.

Cell adhesion and proliferation on RGD-modified recombinant spider silk proteins

Biomaterials ◽  
2012 ◽  
Vol 33 (28) ◽  
pp. 6650-6659 ◽  
Author(s):  
Stefanie Wohlrab ◽  
Susanne Müller ◽  
Andreas Schmidt ◽  
Stefanie Neubauer ◽  
Horst Kessler ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Spider Silk ◽  
Silk Proteins ◽  
Cell Adhesion And Proliferation

Scaffolds in tissue engineering of blood vessels

2010 ◽  
Vol 88 (9) ◽  
pp. 855-873 ◽  
Author(s):  
Divya Pankajakshan ◽  
Devendra K. Agrawal
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Blood Vessels ◽  
Vascular Tissue ◽  
Small Diameter ◽  
Biological Scaffolds ◽  
Hemodynamic Forces ◽  
Biodegradable Scaffolds

Tissue engineering of small diameter (<5 mm) blood vessels is a promising approach for developing viable alternatives to autologous vascular grafts. It involves in vitro seeding of cells onto a scaffold on which the cells attach, proliferate, and differentiate while secreting the components of extracellular matrix that are required for creating the tissue. The scaffold should provide the initial requisite mechanical strength to withstand in vivo hemodynamic forces until vascular smooth muscle cells and fibroblasts reinforce the extracellular matrix of the vessel wall. Hence, the choice of scaffold is crucial for providing guidance cues to the cells to behave in the required manner to produce tissues and organs of the desired shape and size. Several types of scaffolds have been used for the reconstruction of blood vessels. They can be broadly classified as biological scaffolds, decellularized matrices, and polymeric biodegradable scaffolds. This review focuses on the different types of scaffolds that have been designed, developed, and tested for tissue engineering of blood vessels, including use of stem cells in vascular tissue engineering.

scholarly journals Recombinant spider silk proteins as example for supramolecular printable hydrogels

2016 ◽  
Vol 4 ◽  
Author(s):  
J�ngst Tomasz ◽  
Schacht Kristin ◽  
Smolan Willi ◽  
Ewald Andrea ◽  
Scheibel Thomas ◽  
...  
Keyword(s):  
Spider Silk ◽  
Silk Proteins
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