Mating pheromones of Nematoda: olfactory signaling with physiological consequences

2016 ◽  
Vol 38 ◽  
pp. 119-124 ◽  
Author(s):  
Daniel HW Leighton ◽  
Paul W Sternberg
Keyword(s):  
Mating Pheromones

Specific and Common Epitopes in Mating Pheromones of Euplotes raikovi Revealed by Monoclonal Antibodies

1990 ◽  
Vol 37 (3) ◽  
pp. 187-190 ◽  
Author(s):  
PIER LUIGI FIORI ◽  
CRISTINA MICELI ◽  
SIMONA RAFFIONI ◽  
ADRIANA VALLESI
Keyword(s):  
Monoclonal Antibodies ◽  
Mating Pheromones ◽  
Euplotes Raikovi

scholarly journals Activation of an Essential Calcium Signaling Pathway in Saccharomyces cerevisiae by Kch1 and Kch2, Putative Low-Affinity Potassium Transporters

2012 ◽  
Vol 12 (2) ◽  
pp. 204-214 ◽  
Author(s):  
Christopher P. Stefan ◽  
Nannan Zhang ◽  
Takaaki Sokabe ◽  
Alberto Rivetta ◽  
Clifford L. Slayman ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Animal Cell ◽  
Cell Types ◽  
Activation Mechanism ◽  
Homologous Pair ◽  
Content Type ◽  
Mating Pheromones ◽  
Inwardly Rectifying ◽  
Low K ◽  
Mutant Cells

ABSTRACT In the budding yeast Saccharomyces cerevisiae , mating pheromones activate a high-affinity Ca 2+ influx system (HACS) that activates calcineurin and is essential for cell survival. Here we identify extracellular K + and a homologous pair of transmembrane proteins, Kch1 and Kch2 (Prm6), as necessary components of the HACS activation mechanism. Expression of Kch1 and especially Kch2 was strongly induced during the response to mating pheromones. When forcibly overexpressed, Kch1 and Kch2 localized to the plasma membrane and activated HACS in a fashion that depended on extracellular K + but not pheromones. They also promoted growth of trk1 trk2 mutant cells in low K + environments, suggesting they promote K + uptake. Voltage-clamp recordings of protoplasts revealed diminished inward K + currents in kch1 kch2 double-mutant cells relative to the wild type. Conversely, heterologous expression of Kch1 in HEK293T cells caused the appearance of inwardly rectifying K + currents. Collectively, these findings suggest that Kch1 and Kch2 directly promote K + influx and that HACS may electrochemically respond to K + influx in much the same way as the homologous voltage-gated Ca 2+ channels in most animal cell types.

scholarly journals Multiple Signaling Pathways Regulate Yeast Cell Death during the Response to Mating Pheromones

2006 ◽  
Vol 17 (8) ◽  
pp. 3409-3422 ◽  
Author(s):  
Nan-Nan Zhang ◽  
Drew D. Dudgeon ◽  
Saurabh Paliwal ◽  
Andre Levchenko ◽  
Eric Grote ◽  
...  
Keyword(s):  
Cell Death ◽  
Transmembrane Protein ◽  
Yeast Cells ◽  
Third Wave ◽  
Mating Pheromones ◽  
Cell Age ◽  
Clonal Cultures ◽  
High Concentrations ◽  
Multiple Signaling ◽  
Upstream Regulators

Mating pheromones promote cellular differentiation and fusion of yeast cells with those of the opposite mating type. In the absence of a suitable partner, high concentrations of mating pheromones induced rapid cell death in ∼25% of the population of clonal cultures independent of cell age. Rapid cell death required Fig1, a transmembrane protein homologous to PMP-22/EMP/MP20/Claudin proteins, but did not require its Ca2+ influx activity. Rapid cell death also required cell wall degradation, which was inhibited in some surviving cells by the activation of a negative feedback loop involving the MAP kinase Slt2/Mpk1. Mutants lacking Slt2/Mpk1 or its upstream regulators also underwent a second slower wave of cell death that was independent of Fig1 and dependent on much lower concentrations of pheromones. A third wave of cell death that was independent of Fig1 and Slt2/Mpk1 was observed in mutants and conditions that eliminate calcineurin signaling. All three waves of cell death appeared independent of the caspase-like protein Mca1 and lacked certain “hallmarks” of apoptosis. Though all three waves of cell death were preceded by accumulation of reactive oxygen species, mitochondrial respiration was only required for the slowest wave in calcineurin-deficient cells. These findings suggest that yeast cells can die by necrosis-like mechanisms during the response to mating pheromones if essential response pathways are lacking or if mating is attempted in the absence of a partner.

scholarly journals Apparent inhibition of glycoprotein synthesis by S.cerevisiae mating pheromones

FEBS Letters ◽  
1985 ◽  
Vol 184 (2) ◽  
pp. 313-317 ◽  
Author(s):  
Peter Orlean ◽  
Elisabeth Arnold ◽  
Widmar Tanner
Keyword(s):  
Glycoprotein Synthesis ◽  
Mating Pheromones

scholarly journals Asymmetric diversification of mating pheromones in fission yeast

PLoS Biology ◽  
2019 ◽  
Vol 17 (1) ◽  
pp. e3000101 ◽  
Author(s):  
Taisuke Seike ◽  
Chikashi Shimoda ◽  
Hironori Niki
Keyword(s):  
Fission Yeast ◽  
Mating Pheromones

Interspecific actions of ? mating pheromones on the a mating-type cells of three Saccharomyces yeasts

1988 ◽  
Vol 13 (1) ◽  
pp. 25-27 ◽  
Author(s):  
T. Hisatomi ◽  
N. Yanagishima ◽  
A. Sakurai ◽  
H. Kobayashi
Keyword(s):  
Mating Type ◽  
Mating Pheromones ◽  
Saccharomyces Yeasts

scholarly journals The Asexual Yeast Candida glabrata Maintains Distinct a and α Haploid Mating Types

2008 ◽  
Vol 7 (5) ◽  
pp. 848-858 ◽  
Author(s):  
Héloïse Muller ◽  
Christophe Hennequin ◽  
Julien Gallaud ◽  
Bernard Dujon ◽  
Cécile Fairhead
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Type Strain ◽  
Candida Glabrata ◽  
Sexual Cycle ◽  
Mating Types ◽  
Differential Splicing ◽  
Mating Pheromone ◽  
Pheromone Receptor ◽  
Mating Pheromones ◽  
Key Genes

ABSTRACT The genome of the type strain of Candida glabrata (CBS138, ATCC 2001) contains homologs of most of the genes involved in mating in Saccharomyces cerevisiae, starting with the mating pheromone and receptor genes. Only haploid cells are ever isolated, but C. glabrata strains of both mating types are commonly found, the type strain being MATα and most other strains, such as BG2, being MAT a. No sexual cycle has been documented for this species. In order to understand which steps of the mating pathway are defective, we have analyzed the expression of homologs of some of the key genes involved as well as the production of mating pheromones and the organism's sensitivity to artificial pheromones. We show that cells of opposite mating types express both pheromone receptor genes and are insensitive to pheromones. Nonetheless, cells maintain specificity through regulation of the α1 and α2 genes and, more surprisingly, through differential splicing of the a 1 transcript.

scholarly journals Sex-specific mating pheromones in the nematode Panagrellus redivivus

2012 ◽  
Vol 109 (51) ◽  
pp. 20949-20954 ◽  
Author(s):  
A. Choe ◽  
T. Chuman ◽  
S. H. von Reuss ◽  
A. T. Dossey ◽  
J. J. Yim ◽  
...  
Keyword(s):  
Mating Pheromones ◽  
Panagrellus Redivivus
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