Interspecific actions of ? mating pheromones on the a mating-type cells of three Saccharomyces yeasts

1988 ◽  
Vol 13 (1) ◽  
pp. 25-27 ◽  
Author(s):  
T. Hisatomi ◽  
N. Yanagishima ◽  
A. Sakurai ◽  
H. Kobayashi
Keyword(s):  
Mating Type ◽  
Mating Pheromones ◽  
Saccharomyces Yeasts

Phenotypic expression of primary lesions of genetic material in Saccharomyces yeasts

Genome ◽  
1989 ◽  
Vol 31 (2) ◽  
pp. 497-502 ◽  
Author(s):  
Sergey G. Inge-Vechtomov ◽  
Marina V. Repnevskaya
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mating Type ◽  
Uv Light ◽  
Phenotypic Expression ◽  
Genetic Material ◽  
Selective System ◽  
Spontaneous Frequency ◽  
Saccharomyces Yeasts

"Illegitmate" mating of yeasts (α × α), either spontaneous or induced by uv light or ethyl methanesulfanate, in a selective system for "cytoduction" revealed that about 95% of cytoductants expressed their original (α) mating type. Inducing the mating by treating the recipient of cytoplasm with uv light reached two orders of magnitude. An additional copy of MATα in the α recipient almost completely eliminated the effect, which means that nonheritable mating type changes observed are formally recessive and are localized within MATα complex. About 1% of cytoductants obtained were nonmating types and some of them were identified as matα1 mutants. Rad18 mutant as a recipient showed a considerably elevated spontaneous frequency of illegitimate hybridization and cytoduction. The cytoductants also preserved the original mating type. These facts suggest that nonheritable changes of mating type are due to repairable primary (premutational) lesions in MATα genetic material. The significance of these results for understanding the mechanism of nonheritable variability is discussed.Key words: mating type, nonheritable changes, primary lesions, premutational events, Saccharomyces cerevisiae, repair.

scholarly journals Structure and organization of transposable of transposable mating type cassettes in Saccharomyces yeasts.

1980 ◽  
Vol 77 (5) ◽  
pp. 2839-2843 ◽  
Author(s):  
J. N. Strathern ◽  
E. Spatola ◽  
C. McGill ◽  
J. B. Hicks
Keyword(s):  
Mating Type ◽  
Saccharomyces Yeasts

scholarly journals PRECISE MAPPING OF THE HOMOTHALLISM GENES HML AND HMR IN SACCHAROMYCES CEREVISIAE

Genetics ◽  
1980 ◽  
Vol 96 (2) ◽  
pp. 315-320
Author(s):  
Amar J S Klar ◽  
Jean McIndoo ◽  
James B Hicks ◽  
Jeffrey N Strathern
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mating Type ◽  
Control Mechanism ◽  
Negative Control ◽  
Switching Function ◽  
Novel Approach ◽  
Precise Mapping ◽  
Chromosome Iii ◽  
The Right ◽  
Saccharomyces Yeasts

ABSTRACT The HML and HMR loci carry unexpressed copies of MAT  a and MATα information, and a replica of that information is transposed to MAT during mating-type interchange in Saccharomyces yeasts. A negative control mechanism keeps silent the information located at the HML and HMR loci. We mapped these loci by constructing strains in which these loci are expressed. In these strains, the mating type of the segregants is dependent upon the allele at HML and HMR. This novel approach is independent of their switching function. HML is located on the left arm of chromosome III distal to his4 by about 26.8 centimorgans (cM). HMR maps on the right arm of the same chromosome distal to thr4 by about 39.8 cM and proximal to MAL2 by about 1.0 cM. The results allow the exact placement of these loci and are in accord with the observations made by Harashima and Oshima (1976).

scholarly journals EVIDENCE FOR CO-DOMINANCE OF THE HOMOTHALLIC GENES, HMα/hmα AND HM  a/hm  a, IN SACCHAROMYCES YEASTS

Genetics ◽  
1979 ◽  
Vol 93 (1) ◽  
pp. 1-12
Author(s):  
Kenji Arima ◽  
Isamu Takano
Keyword(s):  
Mating Type ◽  
Mating Types ◽  
Type Conversion ◽  
Fusion Of Protoplasts ◽  
Fusion Products ◽  
Saccharomyces Yeasts

ABSTRACT To investigate the dominance and recessiveness of the homothallism genes, HMα/hmα and HMa/hma, for mating-type conversion, we constructed hybrids with various configurations of the homothallic genes by fusion of protoplasts prepared from haploid strains having identical mating types. Eight different combinations of the homothallic genes were tested for their function by observing the mating and sporulation abilities of the fusion products. With few exceptions, nonmating and sporogenous fusion products were obtained from the following combinations: α HO hmα  HMa + α ho hmα hma, α HO hmα HMa + α ho HMα hma, α HO hmα HMa + α ho HMα HMa, α HO HMα hma + a ho hmα hma, a HO HMα hma + a ho hmα HMa and a HO HMα hma + α ho HMα HMa. All the fusion products from the α HO hmα HMa + α ho hmα HMa and a HO HMα hma + a ho HMα hma combinations showed mating types identical to those of the respective haploid strains. These results clearly support the co-dominance of the HMα/hmα and HMa/hma alleles and indicate that the hmα allele has the same function as the HMa allele and that the hma allele has the same function as the HMα allele.

scholarly journals MAPPING OF THE HOMOTHALLIC GENES, HMα AND HM  a, IN SACCHAROMYCES YEASTS

Genetics ◽  
1976 ◽  
Vol 84 (3) ◽  
pp. 437-451
Author(s):  
Satoshi Harashima ◽  
Yasuji Oshima
Keyword(s):  
Mating Type ◽  
The Other ◽  
Type Locus ◽  
Mating Type Locus ◽  
Direct Linkage ◽  
Expected Values ◽  
Chromosome Iii ◽  
The Right ◽  
Saccharomyces Yeasts ◽  
Three Factor

ABSTRACT Two of the three homothallic genes, HMα and HM  a, showed direct linkage to the mating-type locus at approximately 73 and 98 stranes (57 and 65 centimorgans [cM]), respectively, whereas, the other, HO, showed no linkage to 25 standard markers distributed over 17 chromosomes including the mating-type locus. To determine whether the HMα and HM  a loci located on the left or right side of the mating-type locus, equations for three factor analysis of three linked genes were derived. Tetrad data were collected and were compared with expected values by X2 statistics. Calculations indicated that the HMα gene is probably located on the right arm at 95 stranes (65 cM) from the centromere and the HM  a locus at approximately 90 stranes (64 cM) on the left arm of chromosome III.

scholarly journals EVIDENCE OF THE INSENSITIVITY OF THE α-inc ALLELE TO THE FUNCTION OF THE HOMOTHALLIC GENES IN SACCHAROMYCES YEASTS

Genetics ◽  
1979 ◽  
Vol 91 (2) ◽  
pp. 245-254
Author(s):  
Isamu Takano ◽  
Kenji Arima
Keyword(s):  
Cell Size ◽  
Mating Type ◽  
Diploid Cell ◽  
Tetrad Analysis ◽  
Type Allele ◽  
Fusion Of Protoplasts ◽  
Diploid Cells ◽  
Functional Combination ◽  
Fusion Products ◽  
Saccharomyces Yeasts

ABSTRACT The possible function of the α-inc allele (an α mating-type allele that is insensitive to the function of the homothallic gene system) was investigated by means of protoplast fusion. The fusion of protoplasts prepared from haploid strains of α-inc  HO HMα HMa and α ho hmα HMa gave rise mainly to nonmating clones (58 of 64 isolates) and a few clones (six of 64 isolates) showing a mating type. Thirty of the 56 nonmating clones showed the diploid cell size and 28 clones had a larger cell size. Tetrad analysis of the nonmating clones with diploid cell size indicated that they were a/α-inc diploid; the normal α allele in α/α-inc cells was preferentially switched to an a allele. This observation further indicated that the HO/ho HMα/hmα HMa/HMa genotype is effective for the conversion of the α to a and that the inconvertibility of the a-inc allele is due to the insensitivity of the mating-type allele to the functional combination of the homothallic genes. It was suspected that fusion products larger than diploid cells might have been caused by multiple fusion of protoplasts.

scholarly journals FUNCTIONAL EQUIVALENCE AND CO-DOMINANCE OF HOMOTHALLIC GENES HMα/hmα AND HM  a/hm  a IN SACCHAROMYCES YEASTS

Genetics ◽  
1980 ◽  
Vol 95 (4) ◽  
pp. 819-831
Author(s):  
Satoshi Harashima ◽  
Yasuji Oshima
Keyword(s):  
Mating Type ◽  
The Other ◽  
Functional Equivalence ◽  
Single Pair ◽  
Type Conversion ◽  
Chromosome Iii ◽  
Saccharomyces Yeasts

ABSTRACT The specificity of mating type in Saccharomyces yeasts is controlled by a pair of alleles, a and α, on chromosome III. They are mutually interconverted by the function of three kinds of homothallic genes, each consisting of a single pair of alleles, HO/ho, HMα/hmα and HMa/hma. For the a to α conversion, HO HMα HMa, HO hmα HMa and HO hmα hma genotypes are effective; whereas, the α to a conversion occurs in HO HMα HMa, HO HMα hma and HO hmα hma cells. To explain these observations, Naumov and Tolstorukov (1973) and Harashima, Nogi and Oshima (1974) suggested that hma and HMα are functionally equivalent and effective for the α to a conversion in combination with HO; whereas, hmα and HMa are functionally equivalent and effective for the a to α conversion with the function of HO. To test this idea and to compare it with two other possible mechanisms, some of the tetrad segregants from four kinds of a/a/α/α tetraploids homozygous for the HO allele and for one of the HMα/hmα and HMa/hma loci, while heterozygous for the other one with +/+/-/- configuration, were investigated with respect to their thallism by self-sporulation. Results indicated the functional equivalence of both the HMα and hma alleles and the hmα and HMα alleles in mating-type conversion, and the co-dominance of the alleles of each locus. From these findings and other data, we agree with the revision of the nomenclature of the HMα/hmα and HMa/hma genes to HMRa/HMRα and HMLα/HMLa, respectively.

Development of a PCR assay for amplification of mating-type loci of Alternaria spp. and related fungi.

2013 ◽  
Vol 65 (1) ◽  
pp. 69-78 ◽  
Author(s):  
Philipp B. Gannibal ◽  
Igor A. Kazartsev
Keyword(s):  
Mating Type ◽  
Pcr Assay
Sign in / Sign up

Export Citation Format

Share Document