Identification and Characterization of Novel Fusion Proteins in Pediatric Acute Megakaryoblastic Leukemia

2014 ◽  
Vol 14 ◽  
pp. S123-S124
Author(s):  
Jinjun Dang ◽  
Amanda Larson Gedman ◽  
Cary S. Koss ◽  
Suresh Marada ◽  
Stacey K. Ogden ◽  
...  
Keyword(s):  
Fusion Proteins ◽  
Acute Megakaryoblastic Leukemia ◽  
Megakaryoblastic Leukemia ◽  
Identification And Characterization

scholarly journals Clinical impact of genomic characterization of 15 patients with acute megakaryoblastic leukemia–related malignancies

2021 ◽  
Vol 7 (2) ◽  
pp. a005975
Author(s):  
Emilie Lalonde ◽  
Stefan Rentas ◽  
Gerald Wertheim ◽  
Kajia Cao ◽  
Lea F. Surrey ◽  
...  
Keyword(s):  
Clinical Impact ◽  
Acute Megakaryoblastic Leukemia ◽  
Megakaryoblastic Leukemia ◽  
Genomic Characterization

scholarly journals Identification and Characterization of the Three Chitin-Binding Domains within the Multidomain Chitinase Chi92 fromAeromonas hydrophila JP101

2001 ◽  
Vol 67 (11) ◽  
pp. 5100-5106 ◽  
Author(s):  
Mei Li Wu ◽  
Yin Ching Chuang ◽  
Jen Pin Chen ◽  
Chin Shuh Chen ◽  
Ming Chung Chang
Keyword(s):  
Aeromonas Hydrophila ◽  
Fusion Proteins ◽  
Catalytic Domain ◽  
The Third ◽  
Binding Domains ◽  
Hydrolytic Activities ◽  
Cellulose Binding ◽  
Identification And Characterization ◽  
Gst Fusion Proteins

ABSTRACT The gene (chi92) encoding the extracellular chitinase of Aeromonas hydrophila JP101 has been cloned and expressed in Escherichia coli. The mature form of Chi92 is an 842-amino-acid (89.830-kDa) modular enzyme comprised of a family 18 catalytic domain, an unknown-function region (the A region), and three chitin-binding domains (ChBDs; Chi92-N, ChBDCI, and ChBDCII). The C-terminally repeated ChBDs, ChBDCI and ChBDCII, were grouped into family V of cellulose-binding domains on the basis of sequence homology. Chitin binding and enzyme activity studies with C-terminally truncated Chi92 derivatives lacking ChBDs demonstrated that the ChBDs are responsible for its adhesion to unprocessed and colloidal chitins. Further adsorption experiments with glutathione S-transferase (GST) fusion proteins (GST-CI and GST-CICII) demonstrated that a single ChBD (ChBDCI) could promote efficient chitin and cellulose binding. In contrast to the two C-terminal ChBDs, the Chi92-N domain is similar to ChiN of Serratia marcescens ChiA, which has been proposed to participate in chitin binding. A truncated derivative of Chi92 that contained only a catalytic domain and Chi92-N still exhibited insoluble-chitin-binding and hydrolytic activities. Thus, it appears that Chi92 contains Chi92-N as the third ChBD in addition to two ChBDs (ChBDCI and ChBDCII).

Pediatric Acute Megakaryoblastic Leukemia: Multitasking Fusion Proteins and Oncogenic Cooperations

2017 ◽  
Vol 3 (9) ◽  
pp. 631-642 ◽  
Author(s):  
Cécile K. Lopez ◽  
Sébastien Malinge ◽  
Muriel Gaudry ◽  
Olivier A. Bernard ◽  
Thomas Mercher
Keyword(s):  
Fusion Proteins ◽  
Acute Megakaryoblastic Leukemia ◽  
Megakaryoblastic Leukemia
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