STIMULATION OF SOLUBLE GUANYLATE CYCLASE ACTIVITY WITH RIOCIGUAT PROMOTES ANGIOGENESIS AND IMPROVES NEOVASCULARIZATION AFTER HINDLIMB ISCHEMIA

2016 ◽  
Vol 32 (10) ◽  
pp. S231-S232
Author(s):  
W. Dhahri ◽  
S. Dussault ◽  
M. Desjarlais ◽  
R. Mathieu ◽  
A. Rivard
Keyword(s):  
Guanylate Cyclase ◽  
Soluble Guanylate Cyclase ◽  
Cyclase Activity ◽  
Hindlimb Ischemia ◽  
Guanylate Cyclase Activity ◽  
Stimulation Of

Human and rat growth hormones enhance guanylate cyclase activity

1981 ◽  
Vol 240 (2) ◽  
pp. E79-E82
Author(s):  
D. L. Vesely
Keyword(s):  
Growth Hormone ◽  
Guanylate Cyclase ◽  
Soluble Guanylate Cyclase ◽  
Human Growth ◽  
Growth Hormones ◽  
Cyclase Activity ◽  
Kidney Cortex ◽  
Guanylate Cyclase Activity ◽  
Rat Growth ◽  
Stimulation Of

The objective of this investigation was to determine whether physiological levels of growth hormone have part of their mechanism of action through stimulation of guanylate cyclase (EC 4.6.1.2.). Rat and human growth hormones enhanced the activity of soluble guanylate cyclase two- to fourfold in rat gracilis anticus skeletal muscle, liver, lung, heart, pancreas, and kidney cortex at a concentration of 10 nM. Dose-response relationships revealed that more than half-maximal stimulation of guanylate cyclase activity was seen at a concentration as low as 10 nM and nonstimulation of guanylate cyclase activity was seen when the concentration was decreased to 1 nM. Maximal enhancement was seen at 100 nM of growth hormone, and there was no further enhancement when the concentration was increased to the micromolar or millimolar range. Thus, the data in this investigation indicate that at concentrations at which growth hormone is known to cause its growth-promoting effects, growth hormone does cause an enhancement of the activity of the guanylate cyclase-cyclic GMP system.

scholarly journals Liberators of NO exert a dual effect on renin secretion from isolated mouse renal juxtaglomerular cells

1993 ◽  
Vol 265 (2) ◽  
pp. F180-F186 ◽  
Author(s):  
K. Schricker ◽  
A. Kurtz
Keyword(s):  
Guanylate Cyclase ◽  
Soluble Guanylate Cyclase ◽  
Primary Cultures ◽  
Inhibitory Effect ◽  
Extracellular Calcium ◽  
Renin Secretion ◽  
Cyclase Activity ◽  
Guanylate Cyclase Activity ◽  
Cyclic Monophosphate ◽  
Stimulation Of

This study aimed to examine the role of nitric oxide (NO) in the regulation of renin secretion from renal juxtaglomerular (JG) cells. Using primary cultures of mouse renal JG cells, we found that sodium nitroprusside (SNP) and 3-morpholino-sydnonimin-hydrochloride (SIN-1), two structurally different liberators of NO, led to a transient inhibition during the first hour followed by a marked dose-dependent stimulation of renin secretion lasting for an additional 20 h. This stimulatory effect was blunted by methylene blue (50 microM) and was reversible within minutes after removal of the NO liberators. SNP and SIN-1 also stimulated guanylate cyclase activity in the cultures with a maximum within the first hour of incubation. Increasing intracellular guanosine 3',5'-cyclic monophosphate levels by 8-(4-chlorophenylthio)guanosine 3',5'-cyclic monophosphate (100 microM) or by atrial natriuretic peptide (10 nM) decreased basal renin secretion but did not inhibit the effect of SNP. The stimulatory effect of SNP was not related to adenosine 3',5'-cyclic monophosphate levels in the JG cells and was blunted after chelation of extracellular calcium by 2 mM ethylene glycol-bis(beta-amino-ethyl ether)-N,N,N'N'-tetraacetic acid. Taken together, our findings suggest that liberators of NO have two effects on renin secretion from isolated JG cells: an inhibitory effect mediated by stimulation of soluble guanylate cyclase activity and a stimulatory effect mediated by an as yet unknown pathway that requires extracellular calcium.

scholarly journals Methylprednisolone stimulation of guanylate cyclase activity in rat ileal mucosa

1978 ◽  
Vol 74 (5) ◽  
pp. 1062 ◽  
Author(s):  
W.G. Marnane ◽  
A.N. Charney ◽  
E.C. Boedeker ◽  
M. Donowitz
Keyword(s):  
Guanylate Cyclase ◽  
Cyclase Activity ◽  
Ileal Mucosa ◽  
Guanylate Cyclase Activity ◽  
Stimulation Of
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