scholarly journals Effect of 8-substituted cyclic AMP derivatives on soluble guanylate cyclase activity from human platelets.

1981 ◽  
Vol 31 (3) ◽  
pp. 355-359
Author(s):  
Yasuharu SASAKI ◽  
Sachiko IKUKAWA ◽  
Hiroyoshi HIDAKA
Keyword(s):  
Cyclic Amp ◽  
Guanylate Cyclase ◽  
Soluble Guanylate Cyclase ◽  
Human Platelets ◽  
Cyclase Activity ◽  
Guanylate Cyclase Activity ◽  
Cyclic Amp Derivatives

Sex and age differences in soluble guanylate cyclase activity in human platelets

Life Sciences ◽  
1995 ◽  
Vol 58 (5) ◽  
pp. 415-419 ◽  
Author(s):  
Toshio Michimata ◽  
Makoto Imamura ◽  
Haruo Mizuma ◽  
Masami Murakami ◽  
Tokuji Iriuchijima
Keyword(s):  
Age Differences ◽  
Guanylate Cyclase ◽  
Soluble Guanylate Cyclase ◽  
Human Platelets ◽  
Cyclase Activity ◽  
Guanylate Cyclase Activity ◽  
Sex And Age Differences

Changes in guanylate cyclase activity of human platelets in adp-induced aggregation

1991 ◽  
Vol 111 (2) ◽  
pp. 176-179 ◽  
Author(s):  
Yu. Yu. Chirkov ◽  
N. N. Belushkina ◽  
I. A. Tyshchuk ◽  
I. S. Severina
Keyword(s):  
Guanylate Cyclase ◽  
Human Platelets ◽  
Cyclase Activity ◽  
Guanylate Cyclase Activity ◽  
Induced Aggregation

scholarly journals cGMP level that reduces cardiac myocyte O2 consumption is altered in renal hypertension

1997 ◽  
Vol 273 (4) ◽  
pp. H1949-H1955 ◽  
Author(s):  
Michaela Straznicka ◽  
Gary Gong ◽  
James Tse ◽  
Peter M. Scholz ◽  
Harvey R. Weiss
Keyword(s):  
Guanylate Cyclase ◽  
Cardiac Myocyte ◽  
Soluble Guanylate Cyclase ◽  
Renal Hypertension ◽  
Cyclase Activity ◽  
Cgmp Level ◽  
Regression Equations ◽  
Guanylate Cyclase Activity ◽  
New Zealand White Rabbits ◽  
And Control

We tested the hypothesis that cardiac myocytes from hypertensive (one kidney, one clip; 1K,1C) cardiac-hypertrophied rabbits require higher guanosine 3′,5′-cyclic monophosphate (cGMP) to similarly lower O2consumption than control myocytes and that this effect is caused by differences in guanylate cyclase activity. Using isolated myocytes from control and 1K,1C New Zealand White rabbits, we obtained O2 consumption (nl O2 ⋅ min−1 ⋅ 105cells) and cGMP (fmol/105 cells) levels after stimulation of guanylate cyclase with nitroprusside, CO, or guanylin (10−8–10−5M). Soluble guanylate cyclase activity was also determined. Basal cGMP was elevated in 1K,1C vs. control (176 ± 28 vs. 85 ± 13) myocytes. cGMP increased in 1K,1C and control myocytes after stimulation with nitroprusside, CO, and guanylin. Guanylate cyclase activity in 1K,1C vs. control myocytes was not statistically different. Basal O2 consumption in 1K,1C vs. control myocytes was comparable (307 ± 1 vs. 299 ± 22). O2 consumption was similarly decreased when guanylate cyclase was stimulated. Control regression equations correlating cGMP and O2consumption were O2 consumption = −1.46 ⋅ [cGMP] + 444.65 ( r = 0.96) for CO, O2 consumption = −0.58 ⋅ [cGMP] + 328.48 ( r = 0.82) for nitroprusside, and O2 consumption = −1.25 ⋅ [cGMP] + 389.15 ( r = 0.88) for guanylin. The 1K,1C regression equations were O2consumption = −1.36 ⋅ [cGMP] + 537.81 ( r = 0.97) for CO, O2 consumption = −0.23 ⋅ [cGMP] + 307.30 ( r = 0.88) for nitroprusside, and O2 consumption = −1.27 ⋅ [cGMP] + 502.91 ( r = 0.89) for guanylin. These data indicate that 1K,1C hypertrophic myocytes had higher cGMP than controls at every level of O2consumption. This effect was not caused by differences in basal or maximal guanylate cyclase activity.

Human and rat growth hormones enhance guanylate cyclase activity

1981 ◽  
Vol 240 (2) ◽  
pp. E79-E82
Author(s):  
D. L. Vesely
Keyword(s):  
Growth Hormone ◽  
Guanylate Cyclase ◽  
Soluble Guanylate Cyclase ◽  
Human Growth ◽  
Growth Hormones ◽  
Cyclase Activity ◽  
Kidney Cortex ◽  
Guanylate Cyclase Activity ◽  
Rat Growth ◽  
Stimulation Of

The objective of this investigation was to determine whether physiological levels of growth hormone have part of their mechanism of action through stimulation of guanylate cyclase (EC 4.6.1.2.). Rat and human growth hormones enhanced the activity of soluble guanylate cyclase two- to fourfold in rat gracilis anticus skeletal muscle, liver, lung, heart, pancreas, and kidney cortex at a concentration of 10 nM. Dose-response relationships revealed that more than half-maximal stimulation of guanylate cyclase activity was seen at a concentration as low as 10 nM and nonstimulation of guanylate cyclase activity was seen when the concentration was decreased to 1 nM. Maximal enhancement was seen at 100 nM of growth hormone, and there was no further enhancement when the concentration was increased to the micromolar or millimolar range. Thus, the data in this investigation indicate that at concentrations at which growth hormone is known to cause its growth-promoting effects, growth hormone does cause an enhancement of the activity of the guanylate cyclase-cyclic GMP system.

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