061 Extracellular matrix remodeling by granzyme B in aging tissues of apolipoprotein E knockout mice

2011 ◽  
Vol 27 (5) ◽  
pp. S82
Author(s):  
P. Hiebert ◽  
W. Boivin ◽  
T. Abraham ◽  
S. Pazooki ◽  
H. Zhao ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Apolipoprotein E ◽  
Knockout Mice ◽  
Granzyme B ◽  
Extracellular Matrix Remodeling ◽  
Matrix Remodeling ◽  
Apolipoprotein E Knockout Mice

Granzyme B contributes to extracellular matrix remodeling and skin aging in apolipoprotein E knockout mice

2011 ◽  
Vol 46 (6) ◽  
pp. 489-499 ◽  
Author(s):  
Paul R. Hiebert ◽  
Wendy A. Boivin ◽  
Thomas Abraham ◽  
Sara Pazooki ◽  
Hongyan Zhao ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Apolipoprotein E ◽  
Knockout Mice ◽  
Granzyme B ◽  
Skin Aging ◽  
Extracellular Matrix Remodeling ◽  
Matrix Remodeling ◽  
Apolipoprotein E Knockout Mice

scholarly journals Levels of Circulating MMCN-151, a Degradation Product of Mimecan, Reflect Pathological Extracellular Matrix Remodeling in Apolipoprotein E Knockout Mice

2011 ◽  
Vol 6 ◽  
pp. BMI.S7777 ◽  
Author(s):  
N. Barascuk ◽  
E. Vassiliadis ◽  
Q. Zheng ◽  
Y. Wang ◽  
W. Wang ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Apolipoprotein E ◽  
Enzyme Linked Immunosorbent Assay ◽  
Matrix Remodeling ◽  
Limit Of Quantification ◽  
Ecm Remodeling ◽  
Apolipoprotein E Knockout Mice ◽  
Significant Difference ◽  
Apoe Ko Mice ◽  
Apoe Ko

Aim Arterial extracellular matrix (ECM) remodeling by matrix metalloproteinases (MMPs) is one of the major hallmarks of atherosclerosis. Mimecan, also known as osteoglycin has been implicated in the integrity of the ECM. This study assessed the validity of an enzyme-linked immunosorbent assay (ELISA) developed to measure a specific MMP12-derived fragment of mimecan, MMCN-151, in apolipoprotein-E knockout (ApoE-KO) mice. Methods and Results A mouse monoclonal antibody raised against MMCN-151 was used to develop a competitive ELISA. The assay was validated using samples from 20 ApoE-KO and 20 wild type [C57 BL/6] male mice fed a normal or high-fat diet (HFD) for up to 20 weeks. The technical reliability of the assay was established with intra-assay variability <2% and inter-assay variability < 10%. The lowest limit of quantification of MMCN-151 was 0.5 ng/ml. ApoE-KO mice fed a HFD for 20 weeks had four-fold increased circulating levels of MMCN-151 compared to baseline, whereas MMCN-151 levels in control mice on HFD increased two-fold compared with baseline. After 10 weeks of a HFD, a significant difference in MMCN-151 levels was observed between ApoE-KO and control mice ( P = 0.005) and became more significant at 20 weeks ( P = 0.002). Conclusions The newly developed assay is a reliable detector of MMCN-151 levels which ultimately may be useful indicators of arterial remodeling in patients affected by atherosclerotic disease.

scholarly journals Perforin and Granzyme B Have Separate and Distinct Roles during Atherosclerotic Plaque Development in Apolipoprotein E Knockout Mice

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e78939 ◽  
Author(s):  
Paul R. Hiebert ◽  
Wendy A. Boivin ◽  
Hongyan Zhao ◽  
Bruce M. McManus ◽  
David J. Granville
Keyword(s):  
Apolipoprotein E ◽  
Atherosclerotic Plaque ◽  
Knockout Mice ◽  
Granzyme B ◽  
Plaque Development ◽  
Apolipoprotein E Knockout Mice

scholarly journals Extracellular Matrix Remodeling by Human Granzyme B via Cleavage of Vitronectin, Fibronectin, and Laminin

2005 ◽  
Vol 280 (25) ◽  
pp. 23549-23558 ◽  
Author(s):  
Marguerite S. Buzza ◽  
Laura Zamurs ◽  
Jiuru Sun ◽  
Catherina H. Bird ◽  
A. Ian Smith ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Granzyme B ◽  
Extracellular Matrix Remodeling ◽  
Matrix Remodeling

scholarly journals The Dynamic Interaction between Extracellular Matrix Remodeling and Breast Tumor Progression

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1046
Author(s):  
Jorge Martinez ◽  
Patricio C. Smith
Keyword(s):  
Extracellular Matrix ◽  
Type I Collagen ◽  
Cell Metabolism ◽  
Breast Tumors ◽  
Extracellular Matrix Remodeling ◽  
Type I ◽  
Matrix Remodeling ◽  
Desmoplastic Reaction ◽  
The Impact

Desmoplastic tumors correspond to a unique tissue structure characterized by the abnormal deposition of extracellular matrix. Breast tumors are a typical example of this type of lesion, a property that allows its palpation and early detection. Fibrillar type I collagen is a major component of tumor desmoplasia and its accumulation is causally linked to tumor cell survival and metastasis. For many years, the desmoplastic phenomenon was considered to be a reaction and response of the host tissue against tumor cells and, accordingly, designated as “desmoplastic reaction”. This notion has been challenged in the last decades when desmoplastic tissue was detected in breast tissue in the absence of tumor. This finding suggests that desmoplasia is a preexisting condition that stimulates the development of a malignant phenotype. With this perspective, in the present review, we analyze the role of extracellular matrix remodeling in the development of the desmoplastic response. Importantly, during the discussion, we also analyze the impact of obesity and cell metabolism as critical drivers of tissue remodeling during the development of desmoplasia. New knowledge derived from the dynamic remodeling of the extracellular matrix may lead to novel targets of interest for early diagnosis or therapy in the context of breast tumors.

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