Evaluation of selectivity in multimodal anion exchange systems: A priori prediction of protein retention and examination of mobile phase modifier effects

2011 ◽  
Vol 1218 (43) ◽  
pp. 7813-7820 ◽  
Author(s):  
Ying Hou ◽  
Steven M. Cramer
Keyword(s):  
Anion Exchange ◽  
Mobile Phase ◽  
A Priori ◽  
Protein Retention ◽  
Mobile Phase Modifier

Prediction of the Effect of Mobile-Phase Salt Type on Protein Retention and Selectivity in Anion Exchange Systems

2003 ◽  
Vol 75 (14) ◽  
pp. 3563-3572 ◽  
Author(s):  
Nihal Tugcu ◽  
Minghu Song ◽  
Curt M. Breneman ◽  
N. Sukumar ◽  
Kristin P. Bennett ◽  
...  
Keyword(s):  
Anion Exchange ◽  
Mobile Phase ◽  
Protein Retention

Chiral discrimination of phenoxypropionic acid herbicides on teicoplanin phase: Effect of mobile phase modifier

2002 ◽  
Vol 55 (3-4) ◽  
pp. 143-148 ◽  
Author(s):  
Y. C. Guillaume ◽  
T. T. Truong ◽  
J. Millet ◽  
L. Nicod ◽  
C. Guinchard ◽  
...  
Keyword(s):  
Mobile Phase ◽  
Chiral Discrimination ◽  
Phase Effect ◽  
Mobile Phase Modifier ◽  
Acid Herbicides

Empirical relationship between chiral selectivity and mobile phase modifier properties

1999 ◽  
Vol 852 (2) ◽  
pp. 383-394 ◽  
Author(s):  
John A. Blackwell ◽  
Rodger W. Stringham ◽  
Dong Xiang ◽  
Robert E. Waltermire
Keyword(s):  
Mobile Phase ◽  
Empirical Relationship ◽  
Chiral Selectivity ◽  
Mobile Phase Modifier

scholarly journals Relationship between Stationary and Mobile Phase Composition and its Influence on the Retention Time of Some Analytes in Gas Chromatography

2000 ◽  
Vol 18 (4) ◽  
pp. 323-331
Author(s):  
R. Nasuto
Keyword(s):  
Gas Chromatography ◽  
Mobile Phase ◽  
Hydrogen Gas ◽  
Gas Mixture ◽  
Frontal Analysis ◽  
Mobile Phase Composition ◽  
Column Packing ◽  
Chromatographic Process ◽  
Mobile Phase Modifier ◽  
Methanol Vapour

A binary methanol vapour/hydrogen gas mixture has been used as the mobile phase in gas chromatography. Through the use of modified frontal analysis (undertaken just before measurements of the retention of the tested analytes), it has been possible to determine the adsorption isotherm of the mobile phase modifier (methanol) under typical conditions for a chromatographic process. It was found that adsorption of the mobile phase modifier on the column packing surface caused a decrease in the retention times of all the analytes tested. Furthermore, as a result of such adsorption, an increase in the degree of hydrophobization of the column packing surface also occurred, leading to a decrease of the selectivity of the packing.

Sign in / Sign up

Export Citation Format

Share Document