Single laboratory validation of a method for the determination of Bisphenol A, Bisphenol A diglycidyl ether and its derivatives in canned foods by reversed-phase liquid chromatography

2006 ◽  
Vol 1129 (1) ◽  
pp. 145-148 ◽  
Author(s):  
Cuilian Sun ◽  
Lai Peng Leong ◽  
Philip John Barlow ◽  
Sheot Harn Chan ◽  
Bosco Chen Bloodworth
Keyword(s):  
Liquid Chromatography ◽  
Bisphenol A ◽  
Reversed Phase ◽  
Diglycidyl Ether ◽  
Reversed Phase Liquid Chromatography ◽  
Bisphenol A Diglycidyl Ether ◽  
Canned Foods ◽  
Phase Liquid ◽  
Single Laboratory

Sensitive and Rapid Reversed-Phase Liquid Chromatography–Fluorescence Method for Determining Bisphenol A Diglycidyl Ether in Aqueous–Based Food Simulants

1991 ◽  
Vol 74 (6) ◽  
pp. 925-928 ◽  
Author(s):  
P Paseiro Losada ◽  
P López Mahia ◽  
L Vázquez Odériz ◽  
J Simal Lozano ◽  
J Simal Gándara
Keyword(s):  
Liquid Chromatography ◽  
Bisphenol A ◽  
Reversed Phase ◽  
Diglycidyl Ether ◽  
Reversed Phase Liquid Chromatography ◽  
Food Simulants ◽  
Bisphenol A Diglycidyl Ether ◽  
Relative Standard ◽  
Phase Liquid

Abstract A method has been developed for determination of bisphenol A diglycidyl ether (BADGE) in 3 aqueous-based food simulants: water, 15% (v/v) ethanol, and 3% (w/v) acetic acid. BADGE is extracted with C18 cartridges and the extract Is concentrated under a stream of nitrogen. BADGE is quantltated by reversed-phase liquid chromatography with fluorescence detection. Relative precision at 200 μg/L was 3.4%, the detection limit of the method was 0.1 μg/L, and recoveries of spiking concentrations from 1 to 8 μg/L were nearly 100%. Relative standard deviations for the method ranged from 3.5 to 5.9%, depending on the identity of the spiked aqueousbased food simulant.

Determination of Bisphenol A and Bisphenol B Residues in Canned Peeled Tomatoes by Reversed-Phase Liquid Chromatography

2008 ◽  
Vol 56 (22) ◽  
pp. 10633-10637 ◽  
Author(s):  
Lucia Grumetto ◽  
Domenico Montesano ◽  
Serenella Seccia ◽  
Stefania Albrizio ◽  
Francesco Barbato
Keyword(s):  
Liquid Chromatography ◽  
Bisphenol A ◽  
Reversed Phase ◽  
Reversed Phase Liquid Chromatography ◽  
Phase Liquid

scholarly journals Method for the Determination of Aconitum Alkaloids in Dietary Supplements and Raw Materials by Reversed-Phase Liquid Chromatography with Ultraviolet Detection and Confirmation by Tandem Mass Spectrometry: Single-Laboratory Validation

2006 ◽  
Vol 89 (6) ◽  
pp. 1496-1514 ◽  
Author(s):  
Wai-Tong Tang ◽  
Sui-Kay Wong ◽  
Tin-Yau Law ◽  
Kwok-Chu Pang ◽  
Della Sin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Raw Materials ◽  
Reversed Phase ◽  
Tandem Mass ◽  
Reversed Phase Liquid Chromatography ◽  
Aconitum Alkaloids ◽  
Phase Liquid ◽  
Single Laboratory

Abstract A study of single-laboratory validation (SLV) of a reversed-phase liquid chromatography (RP-LC) method was conducted for the determination of diester-diterpene Aconitum alkaloids, viz., aconitine, mesaconitine, and hypaconitine, in a variety of dietary supplements, including single-and multiple-ingredient dry powder extracts, pills, capsules, and raw materials. The Aconitum alkaloids in the samples were extracted by diethyl ether in the presence of ammonia. After cleanup with solid-phase extraction to remove the matrix interferences, the alkaloids were determined by RP-LC with UV detection at 235 nm, and the results were confirmed by tandem mass spectrometry. The linear responses for aconitine, mesaconitine, and hypaconitine based on the present LC system ranged from 0.5 to 200 μg/mL. Relative standard deviations of 2.0 to 6.9% were obtained from duplicate analysis of 6 test materials of different matrixes for the 3 Aconitum alkaloids performed by 2 analysts on 5 different days. The recoveries determined for supplements and raw materials spiked with 3 Aconitum alkaloids at levels of 2.5-10 μg/g were in the range of 86-99%. In view of the attainment of satisfactory results for accuracy, precision, and recovery in the SLV study, it is recommended that the method validation process proceed to a collaborative study.

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