Development of an effective sample preparation approach for proteomic analysis of silkworm eggs using two-dimensional gel electrophoresis and mass spectrometry

2006 ◽  
Vol 1128 (1-2) ◽  
pp. 133-137 ◽  
Author(s):  
X.H. Long ◽  
J.W. Zhu ◽  
Z.H. Mo ◽  
S. Feng ◽  
G. Cheng ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Proteomic Analysis ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis

Proteomic analysis ofCandida magnoliae strains by two-dimensional gel electrophoresis and mass spectrometry

PROTEOMICS ◽  
2003 ◽  
Vol 3 (12) ◽  
pp. 2330-2338 ◽  
Author(s):  
Do-Yup Lee ◽  
Yong-Cheol Park ◽  
Hyo-Jin Kim ◽  
Yeon-Woo Ryu ◽  
Jin-Ho Seo
Keyword(s):  
Mass Spectrometry ◽  
Proteomic Analysis ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis

scholarly journals Two-dimensional gel electrophoresis to identify arcelins from Phaseolus vulgaris with inmuno-proteomic analysis

2018 ◽  
Vol 36 (2) ◽  
pp. 114-119
Author(s):  
Carolina Bernal ◽  
Daynet Sosa ◽  
Iván Galindo-Castro ◽  
Nardy Diez
Keyword(s):  
Mass Spectrometry ◽  
Proteomic Analysis ◽  
Gel Electrophoresis ◽  
Mass Spectrometry Analysis ◽  
Two Dimensional ◽  
Storage Pests ◽  
Molecular Weight Range ◽  
Two Dimensional Gel Electrophoresis ◽  
Breeding Programs ◽  
Spectrometry Analysis

The present study used proteomics to analyze the expression of lectin-like proteins, specifically arcelins, in P. vulgaris cultivar varieties from Venezuela. A PAGE-SDS analysis of 30 commercial accessions of P. vulgaris showed significant differences in the molecular weight range of lectin-like proteins (arcelins). Eight different accessions were selected based on their electroforetic mobility for the proteomic analysis. Arcelin immuno-detection of two dimentional electrophoresed proteins was used to easily display the different arcelin proteomic profiles of the studied accessions. Mass spectrometry analysis confirmed the arcelin nature of these proteins. This is the first report on arcelin evaluation of the Venezuelan germoplasm of P. vulgaris with the aim of enhancing breeding programs by identifying accession materials with resistance to bean storage pests.

Proteome Analysis of CD4+ T Cells Reveals Differentially Expressed Proteins in Infertile Polycystic Ovary Syndrome Patients

2020 ◽  
Vol 20 ◽  
Author(s):  
Fatemeh Nasri ◽  
Maryam Zare ◽  
Mehrnoosh Doroudchi ◽  
Behrouz Gharesi-Fard
Keyword(s):  
Mass Spectrometry ◽  
T Cells ◽  
T Lymphocytes ◽  
Gel Electrophoresis ◽  
Cd4 T Cells ◽  
Polycystic Ovary ◽  
Proteome Analysis ◽  
Two Dimensional ◽  
Ovary Syndrome ◽  
Two Dimensional Gel Electrophoresis

Background: Polycystic ovary syndrome (PCOS) is the most frequent endocrine disorder affecting 6–7% of premenopausal women. Recent studies revealed that the immune system especially CD4+ T helper cells are important in the context PCOS. Proteome analysis of CD4+ T lymphocytes can provide valuable information regarding the biology of these cells in the context of PCOS. Objective: To investigate immune dysregulation in CD4+ T lymphocytes at the protein level in the context of PCOS using two-dimensional gel electrophoresis (2DE) and mass spectrometry (MS). Methods: In the present study, we applied two-dimensional gel electrophoresis / mass spectrometry to identify proteins differentially expressed by peripheral blood CD4+ T cells in ten PCOS women compared with ten healthy women. Western blot technique was used to confirm the identified proteins. Results: Despite the overall proteome similarities, there were significant differences in the expression of seven spots between two groups (P <0.05). Three proteins, namely phosphatidylethanolamine-binding protein 1, proteasome activator complex subunit 1 and triosephosphate isomerase 1 were successfully identified by Mass technique and confirmed by western blot. All characterized proteins were over-expressed in CD4+ T cells from patients compared to CD4+ T cells from controls (P <0.05). In-silico analysis suggested that the over-expressed proteins interact with other proteins involved in cellular metabolism especially glycolysis and ferroptosis pathway. Conclusion: These findings suggest that metabolic adjustments in CD4+ T lymphocytes, which is in favor of increased glycolysis and Th2 differentiation are important in the context of PCOS.

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