Retention modelling in ternary solvent-strength gradient elution reversed-phase chromatography using 30mm columns

2006 ◽  
Vol 1121 (2) ◽  
pp. 219-227 ◽  
Author(s):  
Melvin R. Euerby ◽  
Federico Scannapieco ◽  
Hans-Jürgen Rieger ◽  
Imre Molnar
Keyword(s):  
Gradient Elution ◽  
Reversed Phase ◽  
Reversed Phase Chromatography ◽  
Solvent Strength ◽  
Strength Gradient

High-performance liquid chromatography of pyruvic and alpha-ketoglutaric acids and its application to urine samples.

1977 ◽  
Vol 23 (5) ◽  
pp. 802-805 ◽  
Author(s):  
J C Liao ◽  
N E Hoffman ◽  
J J Barboriak ◽  
D A Roth
Keyword(s):  
Aqueous Solution ◽  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Ammonium Acetate ◽  
Gradient Elution ◽  
Reversed Phase ◽  
Diabetic Patients ◽  
Reversed Phase Chromatography ◽  
Linear Gradient ◽  
Normal Individuals

Abstract Pyruvic and alpha-ketoglutaric acids were quantitatively and rapidly converted to quinoxalones by a new method, to enhance their detectability in the ultraviolet. The quinoxalones were separated by reversed-phase chromatography with use of 10 micronm particles packed in a 30-cm (length) column. An aqueous solution of ammonium acetate and methanol was used for linear gradient elution. The urinary excretion of pyruvic and alpha-ketoglutaric acids by normal individuals, diabetic patients, and patients with renal dysfunction was determined.

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