Analysis and prediction of input multiplicity for the reactive flash separation using reaction-invariant composition variables

2012 ◽  
Vol 90 (11) ◽  
pp. 1856-1870 ◽  
Author(s):  
José Enrique Jaime-Leal ◽  
Adrián Bonilla-Petriciolet ◽  
Juan Gabriel Segovia-Hernández ◽  
Salvador Hernández ◽  
Héctor Hernández-Escoto
Keyword(s):  
Input Multiplicity

Turbomolecular Pumped Electron Microscopy of Rubella Virus (In BHK Celis)

1968 ◽  
Vol 26 ◽  
pp. 204-205
Author(s):  
A. B. Taylor ◽  
G. C. Cole ◽  
M. A. Holcomb ◽  
C. A. Baechler
Keyword(s):  
Electron Microscopy ◽  
Rubella Virus ◽  
Phosphate Buffer ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Basal Medium ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Input Multiplicity ◽  
Baby Hamster Kidney Cells

An aliquot from a continuous fermenter culture of baby hamster kidney cells (BHK-21 Clone PD-4) (Wistar) maintained in Ca free Eagle's Basal Medium containing 2% Kaolin adsorbed fetal calf serum was planted in spinner flasks at 300,000 cells per ml, total volume 600 ml. After equilibration for one day at 35°C to insure that cells were in log phase, the culture was infected with the M-33-AGMK25 BHK-219 strain of rubella at an input multiplicity of about 6 TCID50 per cell. The virus was identified with specific rubella antiserum.Preliminary experiments had shown that such cultures would reach a peak or plateau HA titer of approximately 1:64, 24 hrs after inoculation and would continue to yield virus for 6 to 12 days. One hundred ml aliquot harvests were withdrawn daily and the culture was returned to volume with growth medium and incubation continued. The harvested cells were spun down rapidly at 2500 rpm per 15 mins., fixed in 3.7% gluteraldehyde in Ca free phosphate buffer saline, and post fixed in osmium tetraoxide. After dehydration, the cells were embedded in Epon 812 and cured approximately 20 hrs at 60°C.

Electron Microscopic Study of Penetration of Newcastle Disease Virus into Cells Leading to Formation of Polykaryocytes

1967 ◽  
Vol 2 (1) ◽  
pp. 71-76
Author(s):  
N. MEISELMAN ◽  
A. KOHN ◽  
D. DANON
Keyword(s):  
Epithelial Cells ◽  
Cell Membrane ◽  
Newcastle Disease Virus ◽  
Microscopic Study ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Cell Membranes ◽  
Viral Envelope ◽  
Electron Microscopic ◽  
Input Multiplicity

Treatment of FL or Lu 106 epithelial cells with Newcastle disease virus (NDV) at an input multiplicity of 500 EID50 per cell induces in these cells the formation of polykaryocytes at the end of 2-3 h of contact. Electron micrographs of such NDV-treated monolayers after 2-10 min of incubation show the presence of virions adsorbed to the cell membranes, in vacuoles and with the viral envelope partly fused with the cell membrane. In polykaryocytes induced by NDV, remnants of cell membranes showing numerous breaks may still be present after 3 h.

INPUT-MULTIPLICITY IN LUMPED-PARAMETER SYSTEMS

1985 ◽  
Vol 39 (1-6) ◽  
pp. 309-322 ◽  
Author(s):  
VEMURI BALAKOTAIAH ◽  
DAN LUSS
Keyword(s):  
Lumped Parameter ◽  
Input Multiplicity

Scanning Electron Microscope Reveals Striking Differences in Cytopathic Effects of Equine Herpesvirus (EHV) Strains

1990 ◽  
Vol 48 (3) ◽  
pp. 182-183
Author(s):  
Emmanuel E. Brako ◽  
L. Phaire-Washington ◽  
Allison Brown ◽  
Norma Brako
Keyword(s):  
Viral Infections ◽  
Cultured Cells ◽  
Morphological Changes ◽  
Culture Collection ◽  
Presumptive Diagnosis ◽  
Cell Monolayers ◽  
Cytopathic Effects ◽  
Input Multiplicity ◽  
Scanning Electron

The phenomena of cytopathic effects (CPE) i.e., those virus-induced morphological changes in cultured cells observable at the level of light microscopy are commonly used in presumptive diagnosis of viral infections. The cytomorphological changes are similar for many viruses in the same Family or Genus. For example, in LLC-RK1 cell monolayers both EHV type 1-strain KyD (EHV-1) and EHV type 2-strain LK (EHV-2) cause focal cell rounding and polykaryocytosis which can be observed by phase-contrast microscopy (LM). To determine whether external and internal ultrastructural differences exist in LLC-RK1 cells (American Type Culture Collection # CCL 106) expressing CPE, cells were infected with each virus and processed for scanning electron microscopy (SEM).Monolayer cultures of LLC-RK1 cells on 12 mm glass coverslips were inoculated with EHV-1 or EHV-2 at low input multiplicity of infection. After an hour’s adsorption at 37 C, residual inoculum was removed, and cultures were washed twice with serum-free medium and incubated in growth medium at 37 C.

Chemical Input Multiplicity Facilitates Arithmetical Processing

2004 ◽  
Vol 126 (47) ◽  
pp. 15400-15401 ◽  
Author(s):  
David Margulies ◽  
Galina Melman ◽  
Clifford E. Felder ◽  
Rina Arad-Yellin ◽  
Abraham Shanzer
Keyword(s):  
Input Multiplicity

scholarly journals Replication of Wild-Type and Mutant Human Cytomegalovirus in Life-Extended Human Diploid Fibroblasts

2000 ◽  
Vol 74 (22) ◽  
pp. 10816-10818 ◽  
Author(s):  
Wade A. Bresnahan ◽  
Gretchen E. Hultman ◽  
Thomas Shenk
Keyword(s):  
Human Cytomegalovirus ◽  
Catalytic Subunit ◽  
Growth Defect ◽  
Wild Type ◽  
Diploid Fibroblasts ◽  
Human Diploid Fibroblasts ◽  
Hcmv Replication ◽  
Input Multiplicity ◽  
Human Telomerase ◽  
Derivatives Of

ABSTRACT A cDNA encoding the catalytic subunit of human telomerase was used to generate life-extended derivatives of primary human diploid fibroblasts. The life-extended cells supported efficient human cytomegalovirus (HCMV) replication. A subclone of the life-extended cells was generated containing the HCMV UL82 gene and used to isolate and propagate a virus that exhibited a profound growth defect after infection at a low input multiplicity.

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